Tags

Type your tag names separated by a space and hit enter

Androgen and estrogen receptors and co-regulators levels in endometria from patients with polycystic ovarian syndrome with and without endometrial hyperplasia.
Gynecol Oncol. 2006 Oct; 103(1):307-14.GO

Abstract

OBJECTIVE

To study if the endocrinological status of PCOS women affects the endometrial sensitivity to steroids by evaluating the expression of androgen receptor (AR), estrogen receptor alpha (ERalpha), estrogen receptor beta (ERbeta), co-activators AIB1 and ARA70, and co-repressor NCoR.

METHODS

Gene and/or protein expression of steroid receptors and co-regulators was measured in 17 samples of normal endometrium (NE), 23 PCOS endometrium without treatment (PCOSE), 11 endometria from PCOS women and with endometrial hyperplasia (HPCOSE), and 10 endometria from patients with endometrial hyperplasia (HE), using RT-PCR and/or immunohistochemistry and Western blot.

RESULTS

Gene and protein expression of AR was relatively elevated in PCOSE and HPCOSE compared with NE. A significant increase in ERalpha protein expression was observed in PCOSE, preferentially in the nucleus of endometrial cells, whereas ERbeta gene and protein expression increased gradually from PCOSE to HPCOSE and HE, mainly in the epithelial compartment. Importantly, we found a gradual increase in the ERbeta/ERalpha gene and protein expression ratio in endometria from the four groups of women. AIB1 showed increased nuclear protein expression in PCOSE compared to NE, in the presence of a high expression of ARA70 in all groups. High expression of ARA70 together with a normal expression level of AIB1 was observed in HPCOSE. The cytoplasmic immunostaining of NCoR was similar between the four groups of patients.

CONCLUSION

The PCOS endometrium exhibits a higher sensitivity to steroid action. We can inferred that these alterations could deregulate the transcription of genes involved in the cell cycle, which may lead to the development of endometrial hyperplasia in PCOS women.

Authors+Show Affiliations

Institute of Maternal and Child Research, PO Box 226-3, IDIMI, University of Chile, Santiago, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16677694

Citation

Villavicencio, A, et al. "Androgen and Estrogen Receptors and Co-regulators Levels in Endometria From Patients With Polycystic Ovarian Syndrome With and Without Endometrial Hyperplasia." Gynecologic Oncology, vol. 103, no. 1, 2006, pp. 307-14.
Villavicencio A, Bacallao K, Avellaira C, et al. Androgen and estrogen receptors and co-regulators levels in endometria from patients with polycystic ovarian syndrome with and without endometrial hyperplasia. Gynecol Oncol. 2006;103(1):307-14.
Villavicencio, A., Bacallao, K., Avellaira, C., Gabler, F., Fuentes, A., & Vega, M. (2006). Androgen and estrogen receptors and co-regulators levels in endometria from patients with polycystic ovarian syndrome with and without endometrial hyperplasia. Gynecologic Oncology, 103(1), 307-14.
Villavicencio A, et al. Androgen and Estrogen Receptors and Co-regulators Levels in Endometria From Patients With Polycystic Ovarian Syndrome With and Without Endometrial Hyperplasia. Gynecol Oncol. 2006;103(1):307-14. PubMed PMID: 16677694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Androgen and estrogen receptors and co-regulators levels in endometria from patients with polycystic ovarian syndrome with and without endometrial hyperplasia. AU - Villavicencio,A, AU - Bacallao,K, AU - Avellaira,C, AU - Gabler,F, AU - Fuentes,A, AU - Vega,M, Y1 - 2006/05/03/ PY - 2005/10/05/received PY - 2006/03/02/revised PY - 2006/03/08/accepted PY - 2006/5/9/pubmed PY - 2006/11/3/medline PY - 2006/5/9/entrez SP - 307 EP - 14 JF - Gynecologic oncology JO - Gynecol. Oncol. VL - 103 IS - 1 N2 - OBJECTIVE: To study if the endocrinological status of PCOS women affects the endometrial sensitivity to steroids by evaluating the expression of androgen receptor (AR), estrogen receptor alpha (ERalpha), estrogen receptor beta (ERbeta), co-activators AIB1 and ARA70, and co-repressor NCoR. METHODS: Gene and/or protein expression of steroid receptors and co-regulators was measured in 17 samples of normal endometrium (NE), 23 PCOS endometrium without treatment (PCOSE), 11 endometria from PCOS women and with endometrial hyperplasia (HPCOSE), and 10 endometria from patients with endometrial hyperplasia (HE), using RT-PCR and/or immunohistochemistry and Western blot. RESULTS: Gene and protein expression of AR was relatively elevated in PCOSE and HPCOSE compared with NE. A significant increase in ERalpha protein expression was observed in PCOSE, preferentially in the nucleus of endometrial cells, whereas ERbeta gene and protein expression increased gradually from PCOSE to HPCOSE and HE, mainly in the epithelial compartment. Importantly, we found a gradual increase in the ERbeta/ERalpha gene and protein expression ratio in endometria from the four groups of women. AIB1 showed increased nuclear protein expression in PCOSE compared to NE, in the presence of a high expression of ARA70 in all groups. High expression of ARA70 together with a normal expression level of AIB1 was observed in HPCOSE. The cytoplasmic immunostaining of NCoR was similar between the four groups of patients. CONCLUSION: The PCOS endometrium exhibits a higher sensitivity to steroid action. We can inferred that these alterations could deregulate the transcription of genes involved in the cell cycle, which may lead to the development of endometrial hyperplasia in PCOS women. SN - 0090-8258 UR - https://www.unboundmedicine.com/medline/citation/16677694/Androgen_and_estrogen_receptors_and_co_regulators_levels_in_endometria_from_patients_with_polycystic_ovarian_syndrome_with_and_without_endometrial_hyperplasia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0090-8258(06)00234-4 DB - PRIME DP - Unbound Medicine ER -