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Sensitivity and specificity of nested and real-time PCR for the detection of Pneumocystis jiroveci in clinical specimens.
Diagn Microbiol Infect Dis. 2006 Oct; 56(2):153-60.DM

Abstract

A polymerase chain reaction (PCR)-based test for Pneumocystis jiroveci (formerly Pneumocystis carinii f. sp. hominis) might be an alternative to histologic diagnoses of P. jiroveci pneumonia (PCP). However, previously developed nested PCR methods tend to have low specificities (high false-positive rates). In this study, nested and quantitative real-time PCR methods for the amplification of the P. jiroveci DHPS (dihydropteroate synthase) gene were evaluated in a variety of stored clinical samples from Spain, South Africa, and Brazil. The sensitivities of both assays were high, ranging from 62.5% to 100% depending on the type of specimen. In a subset of 71 microscopically confirmed PCP cases and 70 negative cases, the sensitivities and specificities were 94% and 81% for nested PCR and 94% and 96% for real-time PCR, respectively. Real-time PCR has a statistically significantly better specificity than nested PCR (P = .015) and is likely to generate fewer false positives.

Authors+Show Affiliations

Alvarez-Martínez MJ
Microbiology Department, Hospital Clínic--IDIBAPS, Universitat de Barcelona, Barcelona 08036, Spain.
Miró JM
No affiliation info available
Valls ME
No affiliation info available
Moreno A
No affiliation info available
Rivas PV
No affiliation info available
Solé M
No affiliation info available
Benito N
No affiliation info available
Domingo P
No affiliation info available
Muñoz C
No affiliation info available
Rivera E
No affiliation info available
Zar HJ
No affiliation info available
Wissmann G
No affiliation info available
Diehl AR
No affiliation info available
Prolla JC
No affiliation info available
de Anta MT
No affiliation info available
Gatell JM
No affiliation info available
Wilson PE
No affiliation info available
Meshnick SR
No affiliation info available
Spanish PCP Working Group
No affiliation info available

MeSH

Bronchoalveolar Lavage FluidHumansPneumocystis InfectionsPneumocystis cariniiPolymerase Chain ReactionSensitivity and Specificity

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16678378

Citation

Alvarez-Martínez, Míriam J., et al. "Sensitivity and Specificity of Nested and Real-time PCR for the Detection of Pneumocystis Jiroveci in Clinical Specimens." Diagnostic Microbiology and Infectious Disease, vol. 56, no. 2, 2006, pp. 153-60.
Alvarez-Martínez MJ, Miró JM, Valls ME, et al. Sensitivity and specificity of nested and real-time PCR for the detection of Pneumocystis jiroveci in clinical specimens. Diagn Microbiol Infect Dis. 2006;56(2):153-60.
Alvarez-Martínez, M. J., Miró, J. M., Valls, M. E., Moreno, A., Rivas, P. V., Solé, M., Benito, N., Domingo, P., Muñoz, C., Rivera, E., Zar, H. J., Wissmann, G., Diehl, A. R., Prolla, J. C., de Anta, M. T., Gatell, J. M., Wilson, P. E., & Meshnick, S. R. (2006). Sensitivity and specificity of nested and real-time PCR for the detection of Pneumocystis jiroveci in clinical specimens. Diagnostic Microbiology and Infectious Disease, 56(2), 153-60.
Alvarez-Martínez MJ, et al. Sensitivity and Specificity of Nested and Real-time PCR for the Detection of Pneumocystis Jiroveci in Clinical Specimens. Diagn Microbiol Infect Dis. 2006;56(2):153-60. PubMed PMID: 16678378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitivity and specificity of nested and real-time PCR for the detection of Pneumocystis jiroveci in clinical specimens. AU - Alvarez-Martínez,Míriam J, AU - Miró,José M, AU - Valls,Maria Eugenia, AU - Moreno,Asunción, AU - Rivas,Paula V, AU - Solé,Manel, AU - Benito,Natividad, AU - Domingo,Pere, AU - Muñoz,Carmen, AU - Rivera,Esteban, AU - Zar,Heather J, AU - Wissmann,Gustavo, AU - Diehl,Ada R S, AU - Prolla,João C, AU - de Anta,Maria Teresa Jiménez, AU - Gatell,José M, AU - Wilson,Paul E, AU - Meshnick,Steven R, AU - ,, Y1 - 2006/05/04/ PY - 2005/09/03/received PY - 2006/02/23/revised PY - 2006/03/08/accepted PY - 2006/5/9/pubmed PY - 2007/1/31/medline PY - 2006/5/9/entrez SP - 153 EP - 60 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 56 IS - 2 N2 - A polymerase chain reaction (PCR)-based test for Pneumocystis jiroveci (formerly Pneumocystis carinii f. sp. hominis) might be an alternative to histologic diagnoses of P. jiroveci pneumonia (PCP). However, previously developed nested PCR methods tend to have low specificities (high false-positive rates). In this study, nested and quantitative real-time PCR methods for the amplification of the P. jiroveci DHPS (dihydropteroate synthase) gene were evaluated in a variety of stored clinical samples from Spain, South Africa, and Brazil. The sensitivities of both assays were high, ranging from 62.5% to 100% depending on the type of specimen. In a subset of 71 microscopically confirmed PCP cases and 70 negative cases, the sensitivities and specificities were 94% and 81% for nested PCR and 94% and 96% for real-time PCR, respectively. Real-time PCR has a statistically significantly better specificity than nested PCR (P = .015) and is likely to generate fewer false positives. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/16678378/Sensitivity_and_specificity_of_nested_and_real_time_PCR_for_the_detection_of_Pneumocystis_jiroveci_in_clinical_specimens_ DB - PRIME DP - Unbound Medicine ER -