Tags

Type your tag names separated by a space and hit enter

Dentin matrix protein 1 regulates dentin sialophosphoprotein gene transcription during early odontoblast differentiation.
J Biol Chem 2006; 281(28):19064-71JB

Abstract

Dentin mineralization requires transcriptional mechanisms to induce a cascade of gene expression for progressive development of the odontoblast phenotype. During cytodifferentiation of odontoblasts there is a constant change of actively transcribed genes. Thus, tissue-specific matrix genes that are silenced in early differentiation are expressed during the terminal differentiation process. Dentin sialophosphoprotein (DSPP) is an extracellular matrix, prototypical dentin, and a bone-specific gene, however, the molecular mechanisms by which it is temporally and spatially regulated are not clear. In this report, we demonstrate that dentin matrix protein 1 (DMP1), which is localized in the nucleus during early differentiation of odontoblasts, is able to bind specifically with the DSPP promoter and activate its transcription. We have identified the specific promoter sequence that binds specifically to the carboxyl end of DMP1. The DNA binding domain in DMP1 resides between amino acids 420 and 489. A chromatin immunoprecipitation assay confirmed the in vivo association of DMP1 with the DSPP promoter. Interactions between DMP1 and DSPP promoter thus provide the foundation to understand how DMP1 regulates the expression of the DSPP gene.

Authors+Show Affiliations

Department of Oral Biology, University of Illinois, Chicago, Illinois 60612, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16679514

Citation

Narayanan, Karthikeyan, et al. "Dentin Matrix Protein 1 Regulates Dentin Sialophosphoprotein Gene Transcription During Early Odontoblast Differentiation." The Journal of Biological Chemistry, vol. 281, no. 28, 2006, pp. 19064-71.
Narayanan K, Gajjeraman S, Ramachandran A, et al. Dentin matrix protein 1 regulates dentin sialophosphoprotein gene transcription during early odontoblast differentiation. J Biol Chem. 2006;281(28):19064-71.
Narayanan, K., Gajjeraman, S., Ramachandran, A., Hao, J., & George, A. (2006). Dentin matrix protein 1 regulates dentin sialophosphoprotein gene transcription during early odontoblast differentiation. The Journal of Biological Chemistry, 281(28), pp. 19064-71.
Narayanan K, et al. Dentin Matrix Protein 1 Regulates Dentin Sialophosphoprotein Gene Transcription During Early Odontoblast Differentiation. J Biol Chem. 2006 Jul 14;281(28):19064-71. PubMed PMID: 16679514.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dentin matrix protein 1 regulates dentin sialophosphoprotein gene transcription during early odontoblast differentiation. AU - Narayanan,Karthikeyan, AU - Gajjeraman,Sivakumar, AU - Ramachandran,Amsaveni, AU - Hao,Jianjun, AU - George,Anne, Y1 - 2006/05/05/ PY - 2006/5/9/pubmed PY - 2006/8/22/medline PY - 2006/5/9/entrez SP - 19064 EP - 71 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 281 IS - 28 N2 - Dentin mineralization requires transcriptional mechanisms to induce a cascade of gene expression for progressive development of the odontoblast phenotype. During cytodifferentiation of odontoblasts there is a constant change of actively transcribed genes. Thus, tissue-specific matrix genes that are silenced in early differentiation are expressed during the terminal differentiation process. Dentin sialophosphoprotein (DSPP) is an extracellular matrix, prototypical dentin, and a bone-specific gene, however, the molecular mechanisms by which it is temporally and spatially regulated are not clear. In this report, we demonstrate that dentin matrix protein 1 (DMP1), which is localized in the nucleus during early differentiation of odontoblasts, is able to bind specifically with the DSPP promoter and activate its transcription. We have identified the specific promoter sequence that binds specifically to the carboxyl end of DMP1. The DNA binding domain in DMP1 resides between amino acids 420 and 489. A chromatin immunoprecipitation assay confirmed the in vivo association of DMP1 with the DSPP promoter. Interactions between DMP1 and DSPP promoter thus provide the foundation to understand how DMP1 regulates the expression of the DSPP gene. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/16679514/Dentin_matrix_protein_1_regulates_dentin_sialophosphoprotein_gene_transcription_during_early_odontoblast_differentiation_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=16679514 DB - PRIME DP - Unbound Medicine ER -