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Route of administration (enteral or parenteral) affects the contribution of L-glutamine to de novo L-arginine synthesis in mice: a stable-isotope study.
Am J Physiol Endocrinol Metab 2006; 291(4):E683-90AJ

Abstract

A pathway from enteral L-glutamine as substrate for L-arginine synthesis is suggested by previous studies. L-Glutamine and L-glutamine dipeptides exhibit numerous beneficial effects in experimental and clinical studies. In trauma patients, enteral L-glutamine supply increased plasma L-arginine. The present study was designed to quantify the contribution of L-glutamine to the de novo L-citrulline and L-arginine synthesis in mice when L-glutamine is administered in a high dose of labeled L-glutamine or L-alanyl-L-glutamine by the enteral or parenteral route. For this purpose, male Swiss mice (n = 43) underwent a laparotomy, and catheters were inserted for sampling and infusion. A primed, constant, and continuous infusion of L-alanyl-L-[2-(15)N]glutamine (dipeptide groups) or L-[2-(15)N]glutamine (free L-glutamine groups), simultaneously with L-[ureido-(13)C,(2)H(2)]citrulline and L-[guanidino-(15)N(2),(2)H(2)]arginine, was given (steady-state model). Mice received the L-glutamine tracers intravenously (jugular vein) or enterally (duodenum). Enrichments of metabolites were measured by LC-MS. Arterial L-glutamine concentrations were the highest in the intravenous dipeptide group. L-Glutamine was converted to L-citrulline and L-arginine when L-[2-(15)N]glutamine and L-alanyl-L-[2-(15)N]glutamine were given by enteral or parenteral route. The contribution of L-glutamine to the de novo synthesis of L-citrulline and L-arginine was higher in the enteral groups when compared with the intravenous groups (P < 0.005). Therefore, the route of administration (enteral or parenteral) affects the contribution of L-glutamine, provided as free molecule or dipeptide, to the de novo synthesis of L-arginine in mice.

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  • Publisher Full Text

    • Authors+Show Affiliations

    Boelens PG
    Department of Surgery, Vrije Universiteit. Medical Center, PO Box 7057, 1007 MB Amsterdam, The Netherlands.
    Melis GC
    No affiliation info available
    van Leeuwen PA
    No affiliation info available
    ten Have GA
    No affiliation info available
    Deutz NE
    No affiliation info available

    MeSH

    AnimalsArginineCitrullineEnteral NutritionGlutamineMaleMiceModels, BiologicalNitrogen IsotopesParenteral Nutrition

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16684848

    Citation

    Boelens, Petra G., et al. "Route of Administration (enteral or Parenteral) Affects the Contribution of L-glutamine to De Novo L-arginine Synthesis in Mice: a Stable-isotope Study." American Journal of Physiology. Endocrinology and Metabolism, vol. 291, no. 4, 2006, pp. E683-90.
    Boelens PG, Melis GC, van Leeuwen PA, et al. Route of administration (enteral or parenteral) affects the contribution of L-glutamine to de novo L-arginine synthesis in mice: a stable-isotope study. Am J Physiol Endocrinol Metab. 2006;291(4):E683-90.
    Boelens, P. G., Melis, G. C., van Leeuwen, P. A., ten Have, G. A., & Deutz, N. E. (2006). Route of administration (enteral or parenteral) affects the contribution of L-glutamine to de novo L-arginine synthesis in mice: a stable-isotope study. American Journal of Physiology. Endocrinology and Metabolism, 291(4), pp. E683-90.
    Boelens PG, et al. Route of Administration (enteral or Parenteral) Affects the Contribution of L-glutamine to De Novo L-arginine Synthesis in Mice: a Stable-isotope Study. Am J Physiol Endocrinol Metab. 2006;291(4):E683-90. PubMed PMID: 16684848.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Route of administration (enteral or parenteral) affects the contribution of L-glutamine to de novo L-arginine synthesis in mice: a stable-isotope study. AU - Boelens,Petra G, AU - Melis,Gerdien C, AU - van Leeuwen,Paul A, AU - ten Have,Gabrie A, AU - Deutz,Nicolaas E, Y1 - 2006/05/09/ PY - 2006/5/11/pubmed PY - 2006/10/25/medline PY - 2006/5/11/entrez SP - E683 EP - 90 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 291 IS - 4 N2 - A pathway from enteral L-glutamine as substrate for L-arginine synthesis is suggested by previous studies. L-Glutamine and L-glutamine dipeptides exhibit numerous beneficial effects in experimental and clinical studies. In trauma patients, enteral L-glutamine supply increased plasma L-arginine. The present study was designed to quantify the contribution of L-glutamine to the de novo L-citrulline and L-arginine synthesis in mice when L-glutamine is administered in a high dose of labeled L-glutamine or L-alanyl-L-glutamine by the enteral or parenteral route. For this purpose, male Swiss mice (n = 43) underwent a laparotomy, and catheters were inserted for sampling and infusion. A primed, constant, and continuous infusion of L-alanyl-L-[2-(15)N]glutamine (dipeptide groups) or L-[2-(15)N]glutamine (free L-glutamine groups), simultaneously with L-[ureido-(13)C,(2)H(2)]citrulline and L-[guanidino-(15)N(2),(2)H(2)]arginine, was given (steady-state model). Mice received the L-glutamine tracers intravenously (jugular vein) or enterally (duodenum). Enrichments of metabolites were measured by LC-MS. Arterial L-glutamine concentrations were the highest in the intravenous dipeptide group. L-Glutamine was converted to L-citrulline and L-arginine when L-[2-(15)N]glutamine and L-alanyl-L-[2-(15)N]glutamine were given by enteral or parenteral route. The contribution of L-glutamine to the de novo synthesis of L-citrulline and L-arginine was higher in the enteral groups when compared with the intravenous groups (P < 0.005). Therefore, the route of administration (enteral or parenteral) affects the contribution of L-glutamine, provided as free molecule or dipeptide, to the de novo synthesis of L-arginine in mice. SN - 0193-1849 UR - https://www.unboundmedicine.com/medline/citation/16684848/Route_of_administration__enteral_or_parenteral__affects_the_contribution_of_L_glutamine_to_de_novo_L_arginine_synthesis_in_mice:_a_stable_isotope_study_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.00252.2005?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -