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G-CSF and/or M-CSF accelerate differentiation of bone marrow cells into endothelial progenitor cells in vitro.
Oncol Rep. 2006 Jun; 15(6):1523-7.OR

Abstract

It has been reported that granulocyte-colony stimulating factor (G-CSF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) can mobilize endothelial progenitor cells (EPCs) in bone marrow cells (BMCs) into peripheral blood (PB) in vivo. Previously, we also reported that macrophage-colony stimulating factor (M-CSF) can mobilize EPCs into PB, which results in the rapid recovery of blood flow in induced-ischemia limbs by augmenting the number of intramuscular capillaries in vivo. In the present study, we demonstrate that M-CSF and/or G-CSF can increase EPCs from lineage (CD3, B220, Gr-1, Mac-1, CD11c, Ter119, NK1.1 or CD31)-negative BMCs in vitro. Lineage-negative BMCs were cultured with or without M-CSF and/or G-CSF. Three days after culture with M-CSF and/or G-CSF, the number of Flk-1+/CD45-, Sca-1+/CD45-, CD31+/CD45- or CD146+/CD45- cells increased in comparison with no cytokines. When the cultured BMCs with or without G-CSF and/or M-CSF were intravenously injected into ischemia-induced hindlimbs of mice, the number of intramuscular capillaries in the ischemia-induced legs increased; BMCs cultured with G-CSF and/or M-CSF were more effective than those of cytokine non-treated BMCs. These results suggest that M-CSF and/or G-CSF can induce the differentiation of BMCs into EPCs, even in vitro.

Authors+Show Affiliations

First Department of Pathology, Kansai Medical University, Moriguchi, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16685390

Citation

Zhang, Yuming, et al. "G-CSF And/or M-CSF Accelerate Differentiation of Bone Marrow Cells Into Endothelial Progenitor Cells in Vitro." Oncology Reports, vol. 15, no. 6, 2006, pp. 1523-7.
Zhang Y, Adachi Y, Iwasaki M, et al. G-CSF and/or M-CSF accelerate differentiation of bone marrow cells into endothelial progenitor cells in vitro. Oncol Rep. 2006;15(6):1523-7.
Zhang, Y., Adachi, Y., Iwasaki, M., Minamino, K., Suzuki, Y., Nakano, K., Koike, Y., Mukaide, H., Shigematsu, A., Kiriyama, N., Li, C., & Ikehara, S. (2006). G-CSF and/or M-CSF accelerate differentiation of bone marrow cells into endothelial progenitor cells in vitro. Oncology Reports, 15(6), 1523-7.
Zhang Y, et al. G-CSF And/or M-CSF Accelerate Differentiation of Bone Marrow Cells Into Endothelial Progenitor Cells in Vitro. Oncol Rep. 2006;15(6):1523-7. PubMed PMID: 16685390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - G-CSF and/or M-CSF accelerate differentiation of bone marrow cells into endothelial progenitor cells in vitro. AU - Zhang,Yuming, AU - Adachi,Yasushi, AU - Iwasaki,Masayoshi, AU - Minamino,Keizo, AU - Suzuki,Yasuhiro, AU - Nakano,Keiji, AU - Koike,Yasushi, AU - Mukaide,Hiromi, AU - Shigematsu,Akio, AU - Kiriyama,Naoko, AU - Li,Chunfu, AU - Ikehara,Susumu, PY - 2006/5/11/pubmed PY - 2007/3/9/medline PY - 2006/5/11/entrez SP - 1523 EP - 7 JF - Oncology reports JO - Oncol Rep VL - 15 IS - 6 N2 - It has been reported that granulocyte-colony stimulating factor (G-CSF) and granulocyte-macrophage-colony stimulating factor (GM-CSF) can mobilize endothelial progenitor cells (EPCs) in bone marrow cells (BMCs) into peripheral blood (PB) in vivo. Previously, we also reported that macrophage-colony stimulating factor (M-CSF) can mobilize EPCs into PB, which results in the rapid recovery of blood flow in induced-ischemia limbs by augmenting the number of intramuscular capillaries in vivo. In the present study, we demonstrate that M-CSF and/or G-CSF can increase EPCs from lineage (CD3, B220, Gr-1, Mac-1, CD11c, Ter119, NK1.1 or CD31)-negative BMCs in vitro. Lineage-negative BMCs were cultured with or without M-CSF and/or G-CSF. Three days after culture with M-CSF and/or G-CSF, the number of Flk-1+/CD45-, Sca-1+/CD45-, CD31+/CD45- or CD146+/CD45- cells increased in comparison with no cytokines. When the cultured BMCs with or without G-CSF and/or M-CSF were intravenously injected into ischemia-induced hindlimbs of mice, the number of intramuscular capillaries in the ischemia-induced legs increased; BMCs cultured with G-CSF and/or M-CSF were more effective than those of cytokine non-treated BMCs. These results suggest that M-CSF and/or G-CSF can induce the differentiation of BMCs into EPCs, even in vitro. SN - 1021-335X UR - https://www.unboundmedicine.com/medline/citation/16685390/G_CSF_and/or_M_CSF_accelerate_differentiation_of_bone_marrow_cells_into_endothelial_progenitor_cells_in_vitro_ L2 - https://www.spandidos-publications.com/or/15/6/1523 DB - PRIME DP - Unbound Medicine ER -