TY - JOUR T1 - Method optimization by solid-phase microextraction in combination with gas chromatography with mass spectrometry for analysis of beer volatile fraction. AU - Pinho,Olivia, AU - Ferreira,Isabel M P L V O, AU - Santos,Lúcia H M L M, Y1 - 2006/05/09/ PY - 2006/02/15/received PY - 2006/04/07/revised PY - 2006/04/10/accepted PY - 2006/5/12/pubmed PY - 2006/9/26/medline PY - 2006/5/12/entrez SP - 145 EP - 53 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1121 IS - 2 N2 - A simple and sensitive method for the analysis of beer volatile compounds was optimised using headspace solid-phase microextraction (SPME) and gas chromatography with mass detection. Headspace SPME using a 75 microm Carboxen-polydimethylsiloxane (CAR-PDMS) fiber provided effective sample enrichment and enabled extraction of a wide variety of compounds. The reproducibility depended on the compounds, with a mean value of 1.4% for alcohols, 3.3% for ethers, 6.7% for aldehydes, 3.4% for acids, 1.7% for aromatic compounds, 2.4% for esters, 7.4% for hydrocarbons, 1.8% for alicyclic compounds, and 3.4% for heterocyclic compounds. The optimised methodology can be used to compare volatile profile from different types of beers and eventually to study the evolution of a particular beer during aging. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16687150/Method_optimization_by_solid_phase_microextraction_in_combination_with_gas_chromatography_with_mass_spectrometry_for_analysis_of_beer_volatile_fraction_ DB - PRIME DP - Unbound Medicine ER -