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Geometrical focusing of cells in a microfluidic device: an approach to separate blood plasma.
Biorheology 2006; 43(2):147-59B

Abstract

It is well known that when a suspension of cells flows in small vessels (arterioles or venules), there exists a cell-free layer of a few microns adjacent to the vascular walls. Using an in vitro model, we show experimentally that for a fixed flow rate a geometrical constriction in the flow can artificially enhance the cell-free layer. Also, we show that rapid variation of the geometry coupled to the deformability of the cells can dramatically modify their spatial distribution in the channel. The effects of the constriction geometry, flow rate, suspending fluid viscosity, cell concentration, and cell deformability are studied and the results are interpreted in terms of a model of the hydrodynamic drift of an ellipsoidal cell in a shear flow. We propose a microfluidic application of this focusing effect for separation of the red blood cells from the suspending plasma.

Authors+Show Affiliations

Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16687784

Citation

Faivre, Magalie, et al. "Geometrical Focusing of Cells in a Microfluidic Device: an Approach to Separate Blood Plasma." Biorheology, vol. 43, no. 2, 2006, pp. 147-59.
Faivre M, Abkarian M, Bickraj K, et al. Geometrical focusing of cells in a microfluidic device: an approach to separate blood plasma. Biorheology. 2006;43(2):147-59.
Faivre, M., Abkarian, M., Bickraj, K., & Stone, H. A. (2006). Geometrical focusing of cells in a microfluidic device: an approach to separate blood plasma. Biorheology, 43(2), pp. 147-59.
Faivre M, et al. Geometrical Focusing of Cells in a Microfluidic Device: an Approach to Separate Blood Plasma. Biorheology. 2006;43(2):147-59. PubMed PMID: 16687784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Geometrical focusing of cells in a microfluidic device: an approach to separate blood plasma. AU - Faivre,Magalie, AU - Abkarian,Manouk, AU - Bickraj,Kimberly, AU - Stone,Howard A, PY - 2006/5/12/pubmed PY - 2006/11/11/medline PY - 2006/5/12/entrez SP - 147 EP - 59 JF - Biorheology JO - Biorheology VL - 43 IS - 2 N2 - It is well known that when a suspension of cells flows in small vessels (arterioles or venules), there exists a cell-free layer of a few microns adjacent to the vascular walls. Using an in vitro model, we show experimentally that for a fixed flow rate a geometrical constriction in the flow can artificially enhance the cell-free layer. Also, we show that rapid variation of the geometry coupled to the deformability of the cells can dramatically modify their spatial distribution in the channel. The effects of the constriction geometry, flow rate, suspending fluid viscosity, cell concentration, and cell deformability are studied and the results are interpreted in terms of a model of the hydrodynamic drift of an ellipsoidal cell in a shear flow. We propose a microfluidic application of this focusing effect for separation of the red blood cells from the suspending plasma. SN - 0006-355X UR - https://www.unboundmedicine.com/medline/citation/16687784/Geometrical_focusing_of_cells_in_a_microfluidic_device:_an_approach_to_separate_blood_plasma_ L2 - https://content.iospress.com/openurl?genre=article&issn=0006-355X&volume=43&issue=2&spage=147 DB - PRIME DP - Unbound Medicine ER -