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Differential IgE reactivity to Der p 1 and Der p 2 allergens of Dermatophagoides pteronyssinus in mite-sensitized patients.
J Investig Allergol Clin Immunol. 2006; 16(2):104-9.JI

Abstract

Several studies have shown that the presence of IgE antibodies to house dust mites (HDM), particularly Dermatophagoides pteronyssinus (Dpt), is an important risk factor for asthma. Allergen immunotherapy is indicated for patients with IgE antibodies to clinically relevant allergens. The aims of this study were to analyze the levels of specific serum IgE to Der p 1 and Der p 2 allergens in mite-sensitized atopic patients and to compare them with both in vivo (skin prick test) and in vitro (IgE-ELISA) sensitizations to Dpt crude extract. Forty-seven atopic patients with allergic rhinitis with or without intermittent or persistent mild asthma and positive skin prick test (SPT) to Dpt total extract were studied. Thirty age-matched healthy subjects with negative SPT to HDM were included as controls. Levels of total IgE and Dpt-, Der p 1- and Der p 2-specific IgE were measured by ELISAs in SPT-positive atopic patients and SPT-negative control subjects. Among 47 symptomatic atopic patients, 27 (57.4%) were double positive IgE to Der p 1 and Der p 2 allergens, 3 (6.4%) were single positive IgE to Der p 1, 4 (8.5%) were single positive IgE to Der p 2, and 13 (27.6%) were double negative IgE to both allergens. There was a significant correlation between Der p 1- and Der p 2-specific IgE levels, but not between Der p 1- or Der p 2-IgE levels and SPT results. The double negative IgE patients had the smallest skin test reactions although they showed high mean levels of total serum IgE. Therefore, the knowledge of specific IgE levels to Der p 1 and Der p 2 major allergens might support physicians for indication or follow-up in mite-sensitized patients under allergen-specific immunotherapy. These approaches might be important for obtaining improved safety and efficacy of the current clinical practice of allergen immunotherapy.

Authors+Show Affiliations

Laboratory of Allergy and Clinical Immunology, Biomedical Science Institute, Federal University of Uberlândia, Brazil. taketomi@ufu.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16689183

Citation

Taketomi, E A., et al. "Differential IgE Reactivity to Der P 1 and Der P 2 Allergens of Dermatophagoides Pteronyssinus in Mite-sensitized Patients." Journal of Investigational Allergology & Clinical Immunology, vol. 16, no. 2, 2006, pp. 104-9.
Taketomi EA, Silva DA, Sopelete MC, et al. Differential IgE reactivity to Der p 1 and Der p 2 allergens of Dermatophagoides pteronyssinus in mite-sensitized patients. J Investig Allergol Clin Immunol. 2006;16(2):104-9.
Taketomi, E. A., Silva, D. A., Sopelete, M. C., Gervásio, A. M., Alves, R., & Sung, S. J. (2006). Differential IgE reactivity to Der p 1 and Der p 2 allergens of Dermatophagoides pteronyssinus in mite-sensitized patients. Journal of Investigational Allergology & Clinical Immunology, 16(2), 104-9.
Taketomi EA, et al. Differential IgE Reactivity to Der P 1 and Der P 2 Allergens of Dermatophagoides Pteronyssinus in Mite-sensitized Patients. J Investig Allergol Clin Immunol. 2006;16(2):104-9. PubMed PMID: 16689183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential IgE reactivity to Der p 1 and Der p 2 allergens of Dermatophagoides pteronyssinus in mite-sensitized patients. AU - Taketomi,E A, AU - Silva,D A O, AU - Sopelete,M C, AU - Gervásio,A M, AU - Alves,R, AU - Sung,S J, PY - 2006/5/13/pubmed PY - 2006/10/27/medline PY - 2006/5/13/entrez SP - 104 EP - 9 JF - Journal of investigational allergology & clinical immunology JO - J Investig Allergol Clin Immunol VL - 16 IS - 2 N2 - Several studies have shown that the presence of IgE antibodies to house dust mites (HDM), particularly Dermatophagoides pteronyssinus (Dpt), is an important risk factor for asthma. Allergen immunotherapy is indicated for patients with IgE antibodies to clinically relevant allergens. The aims of this study were to analyze the levels of specific serum IgE to Der p 1 and Der p 2 allergens in mite-sensitized atopic patients and to compare them with both in vivo (skin prick test) and in vitro (IgE-ELISA) sensitizations to Dpt crude extract. Forty-seven atopic patients with allergic rhinitis with or without intermittent or persistent mild asthma and positive skin prick test (SPT) to Dpt total extract were studied. Thirty age-matched healthy subjects with negative SPT to HDM were included as controls. Levels of total IgE and Dpt-, Der p 1- and Der p 2-specific IgE were measured by ELISAs in SPT-positive atopic patients and SPT-negative control subjects. Among 47 symptomatic atopic patients, 27 (57.4%) were double positive IgE to Der p 1 and Der p 2 allergens, 3 (6.4%) were single positive IgE to Der p 1, 4 (8.5%) were single positive IgE to Der p 2, and 13 (27.6%) were double negative IgE to both allergens. There was a significant correlation between Der p 1- and Der p 2-specific IgE levels, but not between Der p 1- or Der p 2-IgE levels and SPT results. The double negative IgE patients had the smallest skin test reactions although they showed high mean levels of total serum IgE. Therefore, the knowledge of specific IgE levels to Der p 1 and Der p 2 major allergens might support physicians for indication or follow-up in mite-sensitized patients under allergen-specific immunotherapy. These approaches might be important for obtaining improved safety and efficacy of the current clinical practice of allergen immunotherapy. SN - 1018-9068 UR - https://www.unboundmedicine.com/medline/citation/16689183/Differential_IgE_reactivity_to_Der_p_1_and_Der_p_2_allergens_of_Dermatophagoides_pteronyssinus_in_mite_sensitized_patients_ L2 - http://www.jiaci.org/issues/vol16issue02/vol16issue02-5.htm DB - PRIME DP - Unbound Medicine ER -