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Magnetic and gold-coated magnetic nanoparticles as a DNA sensor.
Anal Chem. 2006 May 15; 78(10):3234-41.AC

Abstract

In this study, we report the chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles and the immobilization of single-stranded biotinylated oligonucleotides onto these particles. Selected sequences specific to the BRCA1 gene were used as a test platform. The binding of oligonucleotides to these particles was achieved through a streptavidin-biotin bridge via a carbodiimide activation protocol. Particle size and oligonucleotide attachment were confirmed by transmission electron microscopy; oligonucleotide binding was characterized by Fourier transform infrared spectroscopy and hybridization confirmed by fluorescence emission from the fluorophore attached to the target oligonucleotide strand. The rate of hybridization was measured using a spectrofluorometer and a microarray scanner. The rate of hybridization of oligonucleotides bound to the synthesized particles depends on the inorganic support material and its surface chemistry. The rate of hybridization increased concomitantly with the concentration of the probe and the target in the reaction medium. Furthermore, exposure of probe and target oligonucleotide to a combination of target and noncomplementary DNA strand reduced the rate of hybridization, possibly because of steric crowding in the reaction medium and cross-linking between reacting oligonucleotides and the noncomplementary strands. The study undertaken opens several possibilities in bioconjugate attachment to functionalized iron and iron nanocomposite structures for controlled manipulation and handling using magnetic fields.

Authors+Show Affiliations

Department of Agricultural and Biological Engineering/Bindley Biosciences Center, The Pennsylvania State University, University Park, PA 16802, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16689521

Citation

Kouassi, Gilles K., and Joseph Irudayaraj. "Magnetic and Gold-coated Magnetic Nanoparticles as a DNA Sensor." Analytical Chemistry, vol. 78, no. 10, 2006, pp. 3234-41.
Kouassi GK, Irudayaraj J. Magnetic and gold-coated magnetic nanoparticles as a DNA sensor. Anal Chem. 2006;78(10):3234-41.
Kouassi, G. K., & Irudayaraj, J. (2006). Magnetic and gold-coated magnetic nanoparticles as a DNA sensor. Analytical Chemistry, 78(10), 3234-41.
Kouassi GK, Irudayaraj J. Magnetic and Gold-coated Magnetic Nanoparticles as a DNA Sensor. Anal Chem. 2006 May 15;78(10):3234-41. PubMed PMID: 16689521.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Magnetic and gold-coated magnetic nanoparticles as a DNA sensor. AU - Kouassi,Gilles K, AU - Irudayaraj,Joseph, PY - 2006/5/13/pubmed PY - 2007/5/11/medline PY - 2006/5/13/entrez SP - 3234 EP - 41 JF - Analytical chemistry JO - Anal Chem VL - 78 IS - 10 N2 - In this study, we report the chemical synthesis and functionalization of magnetic and gold-coated magnetic nanoparticles and the immobilization of single-stranded biotinylated oligonucleotides onto these particles. Selected sequences specific to the BRCA1 gene were used as a test platform. The binding of oligonucleotides to these particles was achieved through a streptavidin-biotin bridge via a carbodiimide activation protocol. Particle size and oligonucleotide attachment were confirmed by transmission electron microscopy; oligonucleotide binding was characterized by Fourier transform infrared spectroscopy and hybridization confirmed by fluorescence emission from the fluorophore attached to the target oligonucleotide strand. The rate of hybridization was measured using a spectrofluorometer and a microarray scanner. The rate of hybridization of oligonucleotides bound to the synthesized particles depends on the inorganic support material and its surface chemistry. The rate of hybridization increased concomitantly with the concentration of the probe and the target in the reaction medium. Furthermore, exposure of probe and target oligonucleotide to a combination of target and noncomplementary DNA strand reduced the rate of hybridization, possibly because of steric crowding in the reaction medium and cross-linking between reacting oligonucleotides and the noncomplementary strands. The study undertaken opens several possibilities in bioconjugate attachment to functionalized iron and iron nanocomposite structures for controlled manipulation and handling using magnetic fields. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/16689521/Magnetic_and_gold_coated_magnetic_nanoparticles_as_a_DNA_sensor_ L2 - https://doi.org/10.1021/ac051621j DB - PRIME DP - Unbound Medicine ER -