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Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils.
J Ethnopharmacol. 2006 Sep 19; 107(2):240-8.JE

Abstract

Sideritis italica (Miller) Greuter et Burdet is a widespread Lamiacea in the Mediterranean region used in traditional medicine. Essential oils were antibacterial against nine ATCC and as many clinically isolated Gram-positive and Gram-negative bacterial strains. Antibacterial activity was also found against Helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. The antibacterial activity of the oils was expressed as MICs (minimum inhibitory concentrations) and MBCs (minimum bactericidal concentrations). At a concentration between 3.9 and 250 microg/ml the oils showed a significant antibacterial effect against both Gram-negative and Gram-positive bacteria. In particular the ATCC strains Pseudomonas aeruginosa (MIC=3.9 microg/ml and 7.8 for flowerheads and leaves, respectively), Proteus mirabilis (MIC=15.6 and 7.8 microg/ml), Salmonella typhi (MIC=7.8 microg/ml) and Proteus vulgaris (MIC=15.6 microg/ml) were the most inhibited. Only Pseudomonas aeruginosa showed MBC at a concentration between 62.6 and 125 microg/ml. The antioxidant activity of the essential oils was evaluated by two cell free colorimetric methods: ABTS and DMPD; leaf oil is more active (4.29 +/- 0.02 trolox equivalents and 4.53 +/- 0.67 ascorbic acid equivalents by ABTS and DMPD, respectively). Finally the antioxidant activity of the essential oils was also evaluated by their effects on human whole blood leukocytes (WB) and on isolated polymorphonucleate (PMN) chemiluminescence. Comparing the effects of the oils from leaves and flowerheads on both PMN and WB chemiluminescence emission, we found no significant differences. Essential oils showed a dose-dependent and linear inhibitory activity on isolated PMN as well as on WB CL emission when PMA-stimulated. On the contrary, the inhibitory activity on resting cells was nonlinear. Our data represent an answer to the continual demand for new antibiotics and antioxidants for the continuous emergence of antibiotic-resistant strains and the growing interest in the substitution of synthetic antioxidants with natural ones. Furthermore, our finding of antibacterial and antioxidant activities in the essential oils from Sideritis italica flowerheads and leaves validated the use of the plant for medical purposes.

Authors+Show Affiliations

Dipartimento delle Scienze Biologiche, sezione di Biologia Vegetale, Università Federico II, via Foria 223, 80139 Napoli, Italy. adbasile@unina.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16690235

Citation

Basile, Adriana, et al. "Antibacterial and Antioxidant Activities in Sideritis Italica (Miller) Greuter Et Burdet Essential Oils." Journal of Ethnopharmacology, vol. 107, no. 2, 2006, pp. 240-8.
Basile A, Senatore F, Gargano R, et al. Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils. J Ethnopharmacol. 2006;107(2):240-8.
Basile, A., Senatore, F., Gargano, R., Sorbo, S., Del Pezzo, M., Lavitola, A., Ritieni, A., Bruno, M., Spatuzzi, D., Rigano, D., & Vuotto, M. L. (2006). Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils. Journal of Ethnopharmacology, 107(2), 240-8.
Basile A, et al. Antibacterial and Antioxidant Activities in Sideritis Italica (Miller) Greuter Et Burdet Essential Oils. J Ethnopharmacol. 2006 Sep 19;107(2):240-8. PubMed PMID: 16690235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial and antioxidant activities in Sideritis italica (Miller) Greuter et Burdet essential oils. AU - Basile,Adriana, AU - Senatore,Felice, AU - Gargano,Rosalba, AU - Sorbo,Sergio, AU - Del Pezzo,Marisa, AU - Lavitola,Alfredo, AU - Ritieni,Alberto, AU - Bruno,Maurizio, AU - Spatuzzi,Daniela, AU - Rigano,Daniela, AU - Vuotto,Maria Luisa, Y1 - 2006/03/28/ PY - 2005/06/16/received PY - 2006/03/06/revised PY - 2006/03/10/accepted PY - 2006/5/13/pubmed PY - 2007/2/8/medline PY - 2006/5/13/entrez SP - 240 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 107 IS - 2 N2 - Sideritis italica (Miller) Greuter et Burdet is a widespread Lamiacea in the Mediterranean region used in traditional medicine. Essential oils were antibacterial against nine ATCC and as many clinically isolated Gram-positive and Gram-negative bacterial strains. Antibacterial activity was also found against Helicobacter pylori: a dose-dependant inhibition was shown between 5 and 25 microg/ml. The antibacterial activity of the oils was expressed as MICs (minimum inhibitory concentrations) and MBCs (minimum bactericidal concentrations). At a concentration between 3.9 and 250 microg/ml the oils showed a significant antibacterial effect against both Gram-negative and Gram-positive bacteria. In particular the ATCC strains Pseudomonas aeruginosa (MIC=3.9 microg/ml and 7.8 for flowerheads and leaves, respectively), Proteus mirabilis (MIC=15.6 and 7.8 microg/ml), Salmonella typhi (MIC=7.8 microg/ml) and Proteus vulgaris (MIC=15.6 microg/ml) were the most inhibited. Only Pseudomonas aeruginosa showed MBC at a concentration between 62.6 and 125 microg/ml. The antioxidant activity of the essential oils was evaluated by two cell free colorimetric methods: ABTS and DMPD; leaf oil is more active (4.29 +/- 0.02 trolox equivalents and 4.53 +/- 0.67 ascorbic acid equivalents by ABTS and DMPD, respectively). Finally the antioxidant activity of the essential oils was also evaluated by their effects on human whole blood leukocytes (WB) and on isolated polymorphonucleate (PMN) chemiluminescence. Comparing the effects of the oils from leaves and flowerheads on both PMN and WB chemiluminescence emission, we found no significant differences. Essential oils showed a dose-dependent and linear inhibitory activity on isolated PMN as well as on WB CL emission when PMA-stimulated. On the contrary, the inhibitory activity on resting cells was nonlinear. Our data represent an answer to the continual demand for new antibiotics and antioxidants for the continuous emergence of antibiotic-resistant strains and the growing interest in the substitution of synthetic antioxidants with natural ones. Furthermore, our finding of antibacterial and antioxidant activities in the essential oils from Sideritis italica flowerheads and leaves validated the use of the plant for medical purposes. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/16690235/Antibacterial_and_antioxidant_activities_in_Sideritis_italica__Miller__Greuter_et_Burdet_essential_oils_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(06)00163-2 DB - PRIME DP - Unbound Medicine ER -