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Molecular epidemiology of clinical and environmental isolates of the Cryptococcus neoformans species complex reveals a high genetic diversity and the presence of the molecular type VGII mating type a in Colombia.
FEMS Yeast Res. 2006 Jun; 6(4):625-35.FY

Abstract

The aim of this study was to investigate the epidemiological relationships of clinical and environmental isolates of the Cryptococcus neoformans species complex in Colombia. The current study reflects data from 1987 to 2004. In Colombia serotypes A and B are most frequently recovered from patients and the environment. Of the 178 clinical isolates studied, 91.1% were of serotype A, 8.4% serotype B and 0.5% serotype C. Of the 247 environmental isolates, 44.2% were of serotype A, 42.6% serotype B and 13.2% serotype C. No serotype D isolates were isolated. Serotype AD has not been recovered in Colombia. PCR fingerprinting with the primers M13, (GACA)4 and (GTG)5 and URA5 gene restriction fragment length polymorphism analysis grouped the majority of clinical serotype A and environmental serotype B isolates into the molecular types VNI (98.1%) and VGII (100%), respectively. Mating type alpha was determined in 99.3% of serotype A isolates, but 96.6% of serotype B isolates were of mating type a. Similar profiles between clinical and environmental isolates suggest that the patients may have acquired the infection from the environment. The data presented form part of the Colombian contribution to the ongoing global survey of the C. neoformans species complex.

Authors+Show Affiliations

Escandón P
Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.
Sánchez A
No affiliation info available
Martínez M
No affiliation info available
Meyer W
No affiliation info available
Castañeda E
No affiliation info available

MeSH

AnimalsBirdsColombiaCryptococcosisCryptococcus neoformansDNA FingerprintingEnvironmental MicrobiologyFecesGenes, Mating Type, FungalGenetic VariationHumansMolecular EpidemiologyPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSerotypingTrees

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16696659

Citation

Escandón, Patricia, et al. "Molecular Epidemiology of Clinical and Environmental Isolates of the Cryptococcus Neoformans Species Complex Reveals a High Genetic Diversity and the Presence of the Molecular Type VGII Mating Type a in Colombia." FEMS Yeast Research, vol. 6, no. 4, 2006, pp. 625-35.
Escandón P, Sánchez A, Martínez M, et al. Molecular epidemiology of clinical and environmental isolates of the Cryptococcus neoformans species complex reveals a high genetic diversity and the presence of the molecular type VGII mating type a in Colombia. FEMS Yeast Res. 2006;6(4):625-35.
Escandón, P., Sánchez, A., Martínez, M., Meyer, W., & Castañeda, E. (2006). Molecular epidemiology of clinical and environmental isolates of the Cryptococcus neoformans species complex reveals a high genetic diversity and the presence of the molecular type VGII mating type a in Colombia. FEMS Yeast Research, 6(4), 625-35.
Escandón P, et al. Molecular Epidemiology of Clinical and Environmental Isolates of the Cryptococcus Neoformans Species Complex Reveals a High Genetic Diversity and the Presence of the Molecular Type VGII Mating Type a in Colombia. FEMS Yeast Res. 2006;6(4):625-35. PubMed PMID: 16696659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology of clinical and environmental isolates of the Cryptococcus neoformans species complex reveals a high genetic diversity and the presence of the molecular type VGII mating type a in Colombia. AU - Escandón,Patricia, AU - Sánchez,Adriana, AU - Martínez,Marcela, AU - Meyer,Wieland, AU - Castañeda,Elizabeth, PY - 2006/5/16/pubmed PY - 2006/7/29/medline PY - 2006/5/16/entrez SP - 625 EP - 35 JF - FEMS yeast research JO - FEMS Yeast Res VL - 6 IS - 4 N2 - The aim of this study was to investigate the epidemiological relationships of clinical and environmental isolates of the Cryptococcus neoformans species complex in Colombia. The current study reflects data from 1987 to 2004. In Colombia serotypes A and B are most frequently recovered from patients and the environment. Of the 178 clinical isolates studied, 91.1% were of serotype A, 8.4% serotype B and 0.5% serotype C. Of the 247 environmental isolates, 44.2% were of serotype A, 42.6% serotype B and 13.2% serotype C. No serotype D isolates were isolated. Serotype AD has not been recovered in Colombia. PCR fingerprinting with the primers M13, (GACA)4 and (GTG)5 and URA5 gene restriction fragment length polymorphism analysis grouped the majority of clinical serotype A and environmental serotype B isolates into the molecular types VNI (98.1%) and VGII (100%), respectively. Mating type alpha was determined in 99.3% of serotype A isolates, but 96.6% of serotype B isolates were of mating type a. Similar profiles between clinical and environmental isolates suggest that the patients may have acquired the infection from the environment. The data presented form part of the Colombian contribution to the ongoing global survey of the C. neoformans species complex. SN - 1567-1356 UR - https://www.unboundmedicine.com/medline/citation/16696659/Molecular_epidemiology_of_clinical_and_environmental_isolates_of_the_Cryptococcus_neoformans_species_complex_reveals_a_high_genetic_diversity_and_the_presence_of_the_molecular_type_VGII_mating_type_a_in_Colombia_ DB - PRIME DP - Unbound Medicine ER -