Tags

Type your tag names separated by a space and hit enter

Adhesion and cytotoxicity of Bacillus cereus and Bacillus thuringiensis to epithelial cells are FlhA and PlcR dependent, respectively.
Microbes Infect. 2006 May; 8(6):1483-91.MI

Abstract

Some bacteria of the Bacillus cereus group are enteropathogens. The first cells encountered by bacteria following oral contamination of the host are epithelial cells. We studied the capacity of these bacteria to adhere to epithelial cells and the consequences of this interaction. We found that cell adhesion is strain dependent and that a strain mutated in flhA, which encodes a component of flagellum-apparatus formation, is impaired in adhesion, suggesting that flagella are important virulence factors. The bacteria are cytotoxic to epithelial cells and induce substantial cytoplasmic and membrane alterations. However, direct contact between cells and bacteria is not required for cytotoxicity. The determinants of this cytotoxicity are secreted and their expression depends on the pleiotropic regulator PlcR. Adhesion and cytotoxicity of B. cereus to epithelial cells might explain the diarrhea caused by these pathogens. Our findings provide further insight into the pathogenicity of B. cereus group members.

Authors+Show Affiliations

Unité Génétique Microbienne et Environnement, Institut National de la Recherche Agronomique, La Minière, 78285 Guyancourt Cedex, France. nramarao@jouy.inra.frNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16697234

Citation

Ramarao, Nalini, and Didier Lereclus. "Adhesion and Cytotoxicity of Bacillus Cereus and Bacillus Thuringiensis to Epithelial Cells Are FlhA and PlcR Dependent, Respectively." Microbes and Infection, vol. 8, no. 6, 2006, pp. 1483-91.
Ramarao N, Lereclus D. Adhesion and cytotoxicity of Bacillus cereus and Bacillus thuringiensis to epithelial cells are FlhA and PlcR dependent, respectively. Microbes Infect. 2006;8(6):1483-91.
Ramarao, N., & Lereclus, D. (2006). Adhesion and cytotoxicity of Bacillus cereus and Bacillus thuringiensis to epithelial cells are FlhA and PlcR dependent, respectively. Microbes and Infection, 8(6), 1483-91.
Ramarao N, Lereclus D. Adhesion and Cytotoxicity of Bacillus Cereus and Bacillus Thuringiensis to Epithelial Cells Are FlhA and PlcR Dependent, Respectively. Microbes Infect. 2006;8(6):1483-91. PubMed PMID: 16697234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adhesion and cytotoxicity of Bacillus cereus and Bacillus thuringiensis to epithelial cells are FlhA and PlcR dependent, respectively. AU - Ramarao,Nalini, AU - Lereclus,Didier, Y1 - 2006/04/03/ PY - 2005/09/05/received PY - 2005/12/28/revised PY - 2006/01/05/accepted PY - 2006/5/16/pubmed PY - 2006/9/22/medline PY - 2006/5/16/entrez SP - 1483 EP - 91 JF - Microbes and infection JO - Microbes Infect. VL - 8 IS - 6 N2 - Some bacteria of the Bacillus cereus group are enteropathogens. The first cells encountered by bacteria following oral contamination of the host are epithelial cells. We studied the capacity of these bacteria to adhere to epithelial cells and the consequences of this interaction. We found that cell adhesion is strain dependent and that a strain mutated in flhA, which encodes a component of flagellum-apparatus formation, is impaired in adhesion, suggesting that flagella are important virulence factors. The bacteria are cytotoxic to epithelial cells and induce substantial cytoplasmic and membrane alterations. However, direct contact between cells and bacteria is not required for cytotoxicity. The determinants of this cytotoxicity are secreted and their expression depends on the pleiotropic regulator PlcR. Adhesion and cytotoxicity of B. cereus to epithelial cells might explain the diarrhea caused by these pathogens. Our findings provide further insight into the pathogenicity of B. cereus group members. SN - 1286-4579 UR - https://www.unboundmedicine.com/medline/citation/16697234/Adhesion_and_cytotoxicity_of_Bacillus_cereus_and_Bacillus_thuringiensis_to_epithelial_cells_are_FlhA_and_PlcR_dependent_respectively_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1286-4579(06)00073-6 DB - PRIME DP - Unbound Medicine ER -