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Human monoclonal thyroglobulin autoantibodies of high affinity. II. Interaction between thyroglobulin and thyroglobulin autoantibodies of different IgG subclasses.
Autoimmunity. 1991; 11(2):97-105.A

Abstract

The interaction of human thyroglobulin (Tg) autoantibodies of different IgG subclasses with Tg was investigated using four high affinity human monoclonal thyroglobulin (Tg) autoantibodies, secreted by human-mouse hybridomas, of subclasses IgG1 (kappa and lambda) and IgG2 (kappa and lambda) and an IgG4 kappa serum monoclonal Tg antibody. With exception of a low level of interference in binding between one IgG1 lambda Tg antibody and one IgG2 kappa Tg antibody (27% decrease), binding by human monoclonal Tg antibodies of one IgG subclass was unaffected by pre-incubation of 125-I Tg (or Tg on an ELISA plate) with a human monoclonal Tg antibody of a different IgG subclass. Furthermore, preincubation of Tg-coated ELISA plates with an IgG1 human monoclonal Tg antibody had little effect on binding to Tg by IgG2, IgG3 and IgG4 Tg antibodies present in the sera of 6 Hashimoto patients. Comparable observations were made using an IgG2 monoclonal Tg antibody and serum Tg antibodies of subclasses IgG1, IgG3 and IgG4. Binding of an IgG1 kappa Tg antibody was inhibited (> 80%) by pre-incubation of Tg with an IgG1 lambda Tg antibody derived by fusion of lymphocytes from the same Hashimoto patient. In contrast, pre-incubation of Tg with an IgG2 kappa Tg antibody had little effect on subsequent binding by an IgG2 lambda Tg antibody derived from lymphocytes of a different Hashimoto patient.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

RSR Ltd., Pentwyn, Cardiff, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1669739

Citation

Fukuma, N F., et al. "Human Monoclonal Thyroglobulin Autoantibodies of High Affinity. II. Interaction Between Thyroglobulin and Thyroglobulin Autoantibodies of Different IgG Subclasses." Autoimmunity, vol. 11, no. 2, 1991, pp. 97-105.
Fukuma NF, McLachlan SM, Horimoto M, et al. Human monoclonal thyroglobulin autoantibodies of high affinity. II. Interaction between thyroglobulin and thyroglobulin autoantibodies of different IgG subclasses. Autoimmunity. 1991;11(2):97-105.
Fukuma, N. F., McLachlan, S. M., Horimoto, M., Petersen, V. B., & Rees Smith, B. (1991). Human monoclonal thyroglobulin autoantibodies of high affinity. II. Interaction between thyroglobulin and thyroglobulin autoantibodies of different IgG subclasses. Autoimmunity, 11(2), 97-105.
Fukuma NF, et al. Human Monoclonal Thyroglobulin Autoantibodies of High Affinity. II. Interaction Between Thyroglobulin and Thyroglobulin Autoantibodies of Different IgG Subclasses. Autoimmunity. 1991;11(2):97-105. PubMed PMID: 1669739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human monoclonal thyroglobulin autoantibodies of high affinity. II. Interaction between thyroglobulin and thyroglobulin autoantibodies of different IgG subclasses. AU - Fukuma,N F, AU - McLachlan,S M, AU - Horimoto,M, AU - Petersen,V B, AU - Rees Smith,B, PY - 1991/1/1/pubmed PY - 1991/1/1/medline PY - 1991/1/1/entrez SP - 97 EP - 105 JF - Autoimmunity JO - Autoimmunity VL - 11 IS - 2 N2 - The interaction of human thyroglobulin (Tg) autoantibodies of different IgG subclasses with Tg was investigated using four high affinity human monoclonal thyroglobulin (Tg) autoantibodies, secreted by human-mouse hybridomas, of subclasses IgG1 (kappa and lambda) and IgG2 (kappa and lambda) and an IgG4 kappa serum monoclonal Tg antibody. With exception of a low level of interference in binding between one IgG1 lambda Tg antibody and one IgG2 kappa Tg antibody (27% decrease), binding by human monoclonal Tg antibodies of one IgG subclass was unaffected by pre-incubation of 125-I Tg (or Tg on an ELISA plate) with a human monoclonal Tg antibody of a different IgG subclass. Furthermore, preincubation of Tg-coated ELISA plates with an IgG1 human monoclonal Tg antibody had little effect on binding to Tg by IgG2, IgG3 and IgG4 Tg antibodies present in the sera of 6 Hashimoto patients. Comparable observations were made using an IgG2 monoclonal Tg antibody and serum Tg antibodies of subclasses IgG1, IgG3 and IgG4. Binding of an IgG1 kappa Tg antibody was inhibited (> 80%) by pre-incubation of Tg with an IgG1 lambda Tg antibody derived by fusion of lymphocytes from the same Hashimoto patient. In contrast, pre-incubation of Tg with an IgG2 kappa Tg antibody had little effect on subsequent binding by an IgG2 lambda Tg antibody derived from lymphocytes of a different Hashimoto patient.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0891-6934 UR - https://www.unboundmedicine.com/medline/citation/1669739/Human_monoclonal_thyroglobulin_autoantibodies_of_high_affinity__II__Interaction_between_thyroglobulin_and_thyroglobulin_autoantibodies_of_different_IgG_subclasses_ L2 - http://www.tandfonline.com/doi/full/10.3109/08916939109035140 DB - PRIME DP - Unbound Medicine ER -