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Effect of docosahexaenoic acid-rich fish oil supplementation on human leukocyte function.
Clin Nutr. 2006 Dec; 25(6):923-38.CN

Abstract

BACKGROUND

The effect of a docosahexaenoic acid (DHA)-rich fish oil (FO) supplementation on human leukocyte function was investigated.

METHODS

Ten male volunteers were supplemented with 3g/day FO containing 26% eicosapentaenoic acid (EPA, 20:5, n-3) and 54% DHA (22:6, n-3) for 2 months.

RESULTS

FO supplementation changed the fatty acid (FA) composition of leukocytes resulting in an increase of n-3/n-6 ratio from 0.18 to 0.62 in lymphocytes and from 0.15 to 0.70 in neutrophils. DHA-rich FO stimulated an increase in phagocytic activity by 62% and 145% in neutrophils and monocytes, respectively. Neutrophil chemotactic response was increased by 128%. The rate of production of reactive oxygen species by neutrophils was also increased, as it was with lymphocyte proliferation. These changes were partially reversed after a 2-month wash out period. With respect to cytokine production by lymphocytes, interleukin (IL)-4 release was not altered, whereas secretions of IL-10, interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha were raised. These results are in contrast to those described by others using EPA-rich FO supplementation. Lymphocyte pleiotropic gene expression was analyzed by a macroarray technique. Of the analyzed genes (588 in total), 77 were modified by the supplementation. FO supplementation resulted in up-regulation of 6 genes (GATA binding protein 2, IL-6 signal transducer, transforming growth factor alpha, TNF, heat shock 90kDa protein 1-alpha and heat shock protein 70kDa 1A) and a down regulation of 71 genes (92.2% of total genes changed). The largest functional group of altered genes was that related to signaling pathways (22% of the total modified genes).

CONCLUSIONS

Therefore, although EPA and DHA are members of n-3 FA family, changes in the proportion of DHA and EPA exert different effects on neutrophil, monocyte and lymphocyte function, which may be a result of specific changes in gene expression.

Authors+Show Affiliations

Gorjão R
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1524, 05508-900, Butantã, São Paulo, SP, Brazil. renatag@icb.usp.br
Verlengia R
No affiliation info available
Lima TM
No affiliation info available
Soriano FG
No affiliation info available
Boaventura MF
No affiliation info available
Kanunfre CC
No affiliation info available
Peres CM
No affiliation info available
Sampaio SC
No affiliation info available
Otton R
No affiliation info available
Folador A
No affiliation info available
Martins EF
No affiliation info available
Curi TC
No affiliation info available
Portiolli EP
No affiliation info available
Newsholme P
No affiliation info available
Curi R
No affiliation info available

MeSH

AdultCell DivisionDietary SupplementsDocosahexaenoic AcidsDouble-Blind MethodEicosapentaenoic AcidFish OilsGene Expression RegulationHumansInterleukin-8Lipid PeroxidationLymphocytesMaleMembrane LipidsMiddle AgedNeutrophilsPhagocytosisReactive Oxygen SpeciesThiobarbituric Acid Reactive Substancesalpha-Tocopherol

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16697494

Citation

Gorjão, Renata, et al. "Effect of Docosahexaenoic Acid-rich Fish Oil Supplementation On Human Leukocyte Function." Clinical Nutrition (Edinburgh, Scotland), vol. 25, no. 6, 2006, pp. 923-38.
Gorjão R, Verlengia R, Lima TM, et al. Effect of docosahexaenoic acid-rich fish oil supplementation on human leukocyte function. Clin Nutr. 2006;25(6):923-38.
Gorjão, R., Verlengia, R., Lima, T. M., Soriano, F. G., Boaventura, M. F., Kanunfre, C. C., Peres, C. M., Sampaio, S. C., Otton, R., Folador, A., Martins, E. F., Curi, T. C., Portiolli, E. P., Newsholme, P., & Curi, R. (2006). Effect of docosahexaenoic acid-rich fish oil supplementation on human leukocyte function. Clinical Nutrition (Edinburgh, Scotland), 25(6), 923-38.
Gorjão R, et al. Effect of Docosahexaenoic Acid-rich Fish Oil Supplementation On Human Leukocyte Function. Clin Nutr. 2006;25(6):923-38. PubMed PMID: 16697494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of docosahexaenoic acid-rich fish oil supplementation on human leukocyte function. AU - Gorjão,Renata, AU - Verlengia,Rozangela, AU - Lima,Thais Martins de, AU - Soriano,Francisco Garcia, AU - Boaventura,Maria Fernanda Cury, AU - Kanunfre,Carla Christine, AU - Peres,Carmem Maldonado, AU - Sampaio,Sandra Concuzzo, AU - Otton,Rosemari, AU - Folador,Alessandra, AU - Martins,Edgair Fernandes, AU - Curi,Tania Cristina Pithon, AU - Portiolli,Erica Paula, AU - Newsholme,Philip, AU - Curi,Rui, Y1 - 2006/05/11/ PY - 2006/02/07/received PY - 2006/03/10/revised PY - 2006/03/17/accepted PY - 2006/5/16/pubmed PY - 2007/3/14/medline PY - 2006/5/16/entrez SP - 923 EP - 38 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 25 IS - 6 N2 - BACKGROUND: The effect of a docosahexaenoic acid (DHA)-rich fish oil (FO) supplementation on human leukocyte function was investigated. METHODS: Ten male volunteers were supplemented with 3g/day FO containing 26% eicosapentaenoic acid (EPA, 20:5, n-3) and 54% DHA (22:6, n-3) for 2 months. RESULTS: FO supplementation changed the fatty acid (FA) composition of leukocytes resulting in an increase of n-3/n-6 ratio from 0.18 to 0.62 in lymphocytes and from 0.15 to 0.70 in neutrophils. DHA-rich FO stimulated an increase in phagocytic activity by 62% and 145% in neutrophils and monocytes, respectively. Neutrophil chemotactic response was increased by 128%. The rate of production of reactive oxygen species by neutrophils was also increased, as it was with lymphocyte proliferation. These changes were partially reversed after a 2-month wash out period. With respect to cytokine production by lymphocytes, interleukin (IL)-4 release was not altered, whereas secretions of IL-10, interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha were raised. These results are in contrast to those described by others using EPA-rich FO supplementation. Lymphocyte pleiotropic gene expression was analyzed by a macroarray technique. Of the analyzed genes (588 in total), 77 were modified by the supplementation. FO supplementation resulted in up-regulation of 6 genes (GATA binding protein 2, IL-6 signal transducer, transforming growth factor alpha, TNF, heat shock 90kDa protein 1-alpha and heat shock protein 70kDa 1A) and a down regulation of 71 genes (92.2% of total genes changed). The largest functional group of altered genes was that related to signaling pathways (22% of the total modified genes). CONCLUSIONS: Therefore, although EPA and DHA are members of n-3 FA family, changes in the proportion of DHA and EPA exert different effects on neutrophil, monocyte and lymphocyte function, which may be a result of specific changes in gene expression. SN - 0261-5614 UR - https://www.unboundmedicine.com/medline/citation/16697494/Effect_of_docosahexaenoic_acid_rich_fish_oil_supplementation_on_human_leukocyte_function_ DB - PRIME DP - Unbound Medicine ER -