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Maternal intranasal immunization with outer membrane protein P6 maintains specific antibody level of derived offspring.
Vaccine. 2006 Jun 19; 24(25):5294-9.V

Abstract

Acute otitis media is one of the most common infectious diseases in children younger than 2 years of age. Immunological studies in young children have revealed that immature antibody-responses to major pathogens, such as S. pneumoniae, H.influenzae, would cause the vulnerability to upper respiratory tract infections. Thus, it is very important to induce effective protective immunity among children younger than 2 years of age. In this study, we evaluated the capacity of maternal immunization with P6 of H. influenzae to evoke specific antibody to P6 and to transfer it to offspring. We intranasally immunized mother mice with P6 and investigated the induction of specific antibody in sera and breast milk. The specific antibody among offspring delivered by immunized mother was also investigated according to the nursing status to evaluate the importance of breast feedings by immunized mothers. Anti-P6 specific IgG in sera were high at delivery and maintained during nursing periods among P6-immunized mother mice. Anti-P6 specific IgG were predominantly induced in breast milk. IgG subclass induced in sera and breast milk from P6-immunized mother mice were IgG2b, followed by IgG1 and IgG2a subclass. Offspring delivered by P6-immunized mothers had anti-P6 specific IgG in sera at the birth. The levels of anti-P6 specific IgG in sera from offspring breast-fed by P6-immunized mothers were then increased until day 14 and then decreased on day 21. The anti-P6 specific IgG in sera from offspring breast-fed by sham-immunized mothers were rapidly decreased after birth. The current findings strongly suggest that maternal intranasal immunization with P6 would be an attractive strategy against NTHi infections during early childhood. It can supply protective antibodies via transplacental transfer during pregnancy and via breast milk after birth.

Authors+Show Affiliations

Department of Otolaryngology - Head and Neck Surgery, Wakayama Medical University, 811-1, Kimiidera, Wakayama-shi, Wakayama 641-8509, Japan. kazuma@wakayama-med.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16697503

Citation

Yamauchi, Kazuma, et al. "Maternal Intranasal Immunization With Outer Membrane Protein P6 Maintains Specific Antibody Level of Derived Offspring." Vaccine, vol. 24, no. 25, 2006, pp. 5294-9.
Yamauchi K, Hotomi M, Billal DS, et al. Maternal intranasal immunization with outer membrane protein P6 maintains specific antibody level of derived offspring. Vaccine. 2006;24(25):5294-9.
Yamauchi, K., Hotomi, M., Billal, D. S., Suzumoto, M., & Yamanaka, N. (2006). Maternal intranasal immunization with outer membrane protein P6 maintains specific antibody level of derived offspring. Vaccine, 24(25), 5294-9.
Yamauchi K, et al. Maternal Intranasal Immunization With Outer Membrane Protein P6 Maintains Specific Antibody Level of Derived Offspring. Vaccine. 2006 Jun 19;24(25):5294-9. PubMed PMID: 16697503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Maternal intranasal immunization with outer membrane protein P6 maintains specific antibody level of derived offspring. AU - Yamauchi,Kazuma, AU - Hotomi,Muneki, AU - Billal,Dewan S, AU - Suzumoto,Masaki, AU - Yamanaka,Noboru, Y1 - 2006/04/04/ PY - 2005/09/20/received PY - 2006/01/31/revised PY - 2006/03/18/accepted PY - 2006/5/16/pubmed PY - 2006/8/17/medline PY - 2006/5/16/entrez SP - 5294 EP - 9 JF - Vaccine JO - Vaccine VL - 24 IS - 25 N2 - Acute otitis media is one of the most common infectious diseases in children younger than 2 years of age. Immunological studies in young children have revealed that immature antibody-responses to major pathogens, such as S. pneumoniae, H.influenzae, would cause the vulnerability to upper respiratory tract infections. Thus, it is very important to induce effective protective immunity among children younger than 2 years of age. In this study, we evaluated the capacity of maternal immunization with P6 of H. influenzae to evoke specific antibody to P6 and to transfer it to offspring. We intranasally immunized mother mice with P6 and investigated the induction of specific antibody in sera and breast milk. The specific antibody among offspring delivered by immunized mother was also investigated according to the nursing status to evaluate the importance of breast feedings by immunized mothers. Anti-P6 specific IgG in sera were high at delivery and maintained during nursing periods among P6-immunized mother mice. Anti-P6 specific IgG were predominantly induced in breast milk. IgG subclass induced in sera and breast milk from P6-immunized mother mice were IgG2b, followed by IgG1 and IgG2a subclass. Offspring delivered by P6-immunized mothers had anti-P6 specific IgG in sera at the birth. The levels of anti-P6 specific IgG in sera from offspring breast-fed by P6-immunized mothers were then increased until day 14 and then decreased on day 21. The anti-P6 specific IgG in sera from offspring breast-fed by sham-immunized mothers were rapidly decreased after birth. The current findings strongly suggest that maternal intranasal immunization with P6 would be an attractive strategy against NTHi infections during early childhood. It can supply protective antibodies via transplacental transfer during pregnancy and via breast milk after birth. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/16697503/Maternal_intranasal_immunization_with_outer_membrane_protein_P6_maintains_specific_antibody_level_of_derived_offspring_ DB - PRIME DP - Unbound Medicine ER -