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Antioxidative enzymes from chloroplasts, mitochondria, and peroxisomes during leaf senescence of nodulated pea plants.
J Exp Bot. 2006; 57(8):1747-58.JE

Abstract

In this work the influence of the nodulation of pea (Pisum sativum L.) plants on the oxidative metabolism of different leaf organelles from young and senescent plants was studied. Chloroplasts, mitochondria, and peroxisomes were purified from leaves of nitrate-fed and Rhizobium leguminosarum-nodulated pea plants at two developmental stages (young and senescent plants). In these cell organelles, the activity of the ascorbate-glutathione cycle enzymes ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR), and the ascorbate and glutathione contents were determined. In addition, the total superoxide dismutase (SOD) activity, the pattern of mitochondrial and peroxisomal NADPH-generating dehydrogenases, some of the peroxisomal photorespiratory enzymes, the glyoxylate cycle and oxidative metabolism enzymes were also analysed in these organelles. Results obtained on the metabolism of cell organelles indicate that nodulation with Rhizobium accelerates senescence in pea leaves. A considerable decrease of the ascorbate content of chloroplasts, mitochondria, and peroxisomes was found, and in these conditions a metabolic conversion of leaf peroxisomes into glyoxysomes, characteristic of leaf senescence, took place.

Authors+Show Affiliations

Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080 Granada, Spain. jmpalma@eez.csic.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16698815

Citation

Palma, José M., et al. "Antioxidative Enzymes From Chloroplasts, Mitochondria, and Peroxisomes During Leaf Senescence of Nodulated Pea Plants." Journal of Experimental Botany, vol. 57, no. 8, 2006, pp. 1747-58.
Palma JM, Jiménez A, Sandalio LM, et al. Antioxidative enzymes from chloroplasts, mitochondria, and peroxisomes during leaf senescence of nodulated pea plants. J Exp Bot. 2006;57(8):1747-58.
Palma, J. M., Jiménez, A., Sandalio, L. M., Corpas, F. J., Lundqvist, M., Gómez, M., Sevilla, F., & del Río, L. A. (2006). Antioxidative enzymes from chloroplasts, mitochondria, and peroxisomes during leaf senescence of nodulated pea plants. Journal of Experimental Botany, 57(8), 1747-58.
Palma JM, et al. Antioxidative Enzymes From Chloroplasts, Mitochondria, and Peroxisomes During Leaf Senescence of Nodulated Pea Plants. J Exp Bot. 2006;57(8):1747-58. PubMed PMID: 16698815.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidative enzymes from chloroplasts, mitochondria, and peroxisomes during leaf senescence of nodulated pea plants. AU - Palma,José M, AU - Jiménez,Ana, AU - Sandalio,Luisa M, AU - Corpas,Francisco J, AU - Lundqvist,Marianne, AU - Gómez,Manuel, AU - Sevilla,Francisca, AU - del Río,Luis A, Y1 - 2006/05/12/ PY - 2006/5/16/pubmed PY - 2006/10/5/medline PY - 2006/5/16/entrez SP - 1747 EP - 58 JF - Journal of experimental botany JO - J Exp Bot VL - 57 IS - 8 N2 - In this work the influence of the nodulation of pea (Pisum sativum L.) plants on the oxidative metabolism of different leaf organelles from young and senescent plants was studied. Chloroplasts, mitochondria, and peroxisomes were purified from leaves of nitrate-fed and Rhizobium leguminosarum-nodulated pea plants at two developmental stages (young and senescent plants). In these cell organelles, the activity of the ascorbate-glutathione cycle enzymes ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR), and the ascorbate and glutathione contents were determined. In addition, the total superoxide dismutase (SOD) activity, the pattern of mitochondrial and peroxisomal NADPH-generating dehydrogenases, some of the peroxisomal photorespiratory enzymes, the glyoxylate cycle and oxidative metabolism enzymes were also analysed in these organelles. Results obtained on the metabolism of cell organelles indicate that nodulation with Rhizobium accelerates senescence in pea leaves. A considerable decrease of the ascorbate content of chloroplasts, mitochondria, and peroxisomes was found, and in these conditions a metabolic conversion of leaf peroxisomes into glyoxysomes, characteristic of leaf senescence, took place. SN - 0022-0957 UR - https://www.unboundmedicine.com/medline/citation/16698815/Antioxidative_enzymes_from_chloroplasts_mitochondria_and_peroxisomes_during_leaf_senescence_of_nodulated_pea_plants_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erj191 DB - PRIME DP - Unbound Medicine ER -