Tags

Type your tag names separated by a space and hit enter

The role of immunohistochemistry for smooth-muscle actin, p63, CD10 and cytokeratin 14 in the differential diagnosis of papillary lesions of the breast.
J Clin Pathol. 2007 Mar; 60(3):315-20.JC

Abstract

BACKGROUND

Histological differentiation of mammary papillary lesions can be difficult. The evaluation of myoepithelial cells can be helpful, with benign papilloma showing a continuous myoepithelial cell layer, which becomes attenuated or absent in malignant papillary lesions.

METHODS

A large series of 100 papillomas (28 papillomas with florid epithelial hyperplasia) and 68 papillary carcinomas (9 invasive, 44 in situ, and 15 ductal carcinomas in situ (DCIS) involving papillomas) of the breast were stained for myoepithelial cells by immunohistochemistry using antibodies to smooth-muscle actin (SMA), p63, CD10 and cytokeratin (CK) 14.

RESULTS

In the papillomas, using these four antibodies, myoepithelial cells were positive in 88%, 99%, 91% and 95% of cases, respectively, with SMA showing marked stromal component cell staining and CD10 showing epithelial and stromal staining. CK14 also showed epithelial staining in 71% of papillomas and 96% of papillomas with florid epithelial hyperplasia. In the papillary carcinomas, 36 (53%) cases showed staining of myoepithelial cells that were scattered, discontinuous and diminished in number and the remaining 32 (47%) cases did not show myoepithelial cells. Invasive papillary carcinoma has the lowest proportion (33%) with myoepithelial cells, and DCIS involving papillomas had the highest proportion (87%).

CONCLUSIONS

p63 had the highest sensitivity and did not cross-react with stromal cells and only rarely with epithelial cells. CK14 has the added ability to distinguish between florid epithelial hyperplasia involving papilloma and DCIS involving papillomas. CK14 and p63 may be used as an adjunct in assessing difficult papillary lesions of the breast.

Authors+Show Affiliations

Department of Anatomical and Cellular Pathology, Prince of Wales Hospital, Chinese University of Hong Kong, Hong Kong SAR, China. garytse@cuhk.edu.hkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16698948

Citation

Tse, G M K., et al. "The Role of Immunohistochemistry for Smooth-muscle Actin, P63, CD10 and Cytokeratin 14 in the Differential Diagnosis of Papillary Lesions of the Breast." Journal of Clinical Pathology, vol. 60, no. 3, 2007, pp. 315-20.
Tse GM, Tan PH, Lui PC, et al. The role of immunohistochemistry for smooth-muscle actin, p63, CD10 and cytokeratin 14 in the differential diagnosis of papillary lesions of the breast. J Clin Pathol. 2007;60(3):315-20.
Tse, G. M., Tan, P. H., Lui, P. C., Gilks, C. B., Poon, C. S., Ma, T. K., Law, B. K., & Lam, W. W. (2007). The role of immunohistochemistry for smooth-muscle actin, p63, CD10 and cytokeratin 14 in the differential diagnosis of papillary lesions of the breast. Journal of Clinical Pathology, 60(3), 315-20.
Tse GM, et al. The Role of Immunohistochemistry for Smooth-muscle Actin, P63, CD10 and Cytokeratin 14 in the Differential Diagnosis of Papillary Lesions of the Breast. J Clin Pathol. 2007;60(3):315-20. PubMed PMID: 16698948.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of immunohistochemistry for smooth-muscle actin, p63, CD10 and cytokeratin 14 in the differential diagnosis of papillary lesions of the breast. AU - Tse,G M K, AU - Tan,P-H, AU - Lui,P C W, AU - Gilks,C B, AU - Poon,C S P, AU - Ma,T K F, AU - Law,B K B, AU - Lam,W W M, Y1 - 2006/05/12/ PY - 2006/5/16/pubmed PY - 2007/5/2/medline PY - 2006/5/16/entrez SP - 315 EP - 20 JF - Journal of clinical pathology JO - J Clin Pathol VL - 60 IS - 3 N2 - BACKGROUND: Histological differentiation of mammary papillary lesions can be difficult. The evaluation of myoepithelial cells can be helpful, with benign papilloma showing a continuous myoepithelial cell layer, which becomes attenuated or absent in malignant papillary lesions. METHODS: A large series of 100 papillomas (28 papillomas with florid epithelial hyperplasia) and 68 papillary carcinomas (9 invasive, 44 in situ, and 15 ductal carcinomas in situ (DCIS) involving papillomas) of the breast were stained for myoepithelial cells by immunohistochemistry using antibodies to smooth-muscle actin (SMA), p63, CD10 and cytokeratin (CK) 14. RESULTS: In the papillomas, using these four antibodies, myoepithelial cells were positive in 88%, 99%, 91% and 95% of cases, respectively, with SMA showing marked stromal component cell staining and CD10 showing epithelial and stromal staining. CK14 also showed epithelial staining in 71% of papillomas and 96% of papillomas with florid epithelial hyperplasia. In the papillary carcinomas, 36 (53%) cases showed staining of myoepithelial cells that were scattered, discontinuous and diminished in number and the remaining 32 (47%) cases did not show myoepithelial cells. Invasive papillary carcinoma has the lowest proportion (33%) with myoepithelial cells, and DCIS involving papillomas had the highest proportion (87%). CONCLUSIONS: p63 had the highest sensitivity and did not cross-react with stromal cells and only rarely with epithelial cells. CK14 has the added ability to distinguish between florid epithelial hyperplasia involving papilloma and DCIS involving papillomas. CK14 and p63 may be used as an adjunct in assessing difficult papillary lesions of the breast. SN - 0021-9746 UR - https://www.unboundmedicine.com/medline/citation/16698948/The_role_of_immunohistochemistry_for_smooth_muscle_actin_p63_CD10_and_cytokeratin_14_in_the_differential_diagnosis_of_papillary_lesions_of_the_breast_ L2 - https://jcp.bmj.com/lookup/pmidlookup?view=long&pmid=16698948 DB - PRIME DP - Unbound Medicine ER -