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Deformation and nano-rheology of red blood cells: an AFM investigation.

Abstract

Interaction forces, deformation and nano-rheology of individual red blood cells in physiologically relevant solution conditions have been determined by colloid probe atomic force microscopy (AFM). On approach of the physically immobilised cell and silica glass spherical probe surfaces, deformation of the red blood cell was observed in the force curves. At low levels of deformation, spring constants were determined in the range 3-6 m Nm(-1), whereas for higher levels of deformation, the forces increase non-linearly and on retraction, significant force curve hysteresis is observed (i.e. lower forces upon retraction). The extent of force curve hysteresis was dependent on both the drive velocity and loading force, typical of a viscoelastic system. The response of the red blood cell has been described by viscoelastic theory, where the short and long time scale elastic moduli and relaxation times are determined, i.e. the cell's nano-rheological properties elucidated. In addition to a time independent elastic modulus of 4.0 x 10(3)Nm(-2) at low levels of deformation, time-dependent elastic moduli ranges are observed (3.5 x 10(4) to 5.5 x 10(4)Nm(-2) at intermediate levels of deformation and 1.5 x 10(5) to 3.0 x 10(5)Nm(-2) at higher levels of deformation). That is, one elastic and more than one viscoelastic response to the red blood cell deformation is evident, which is considered to reflect the cellular structure.

Authors+Show Affiliations

Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA 5095, Australia.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16701986

Citation

Bremmell, Kristen E., et al. "Deformation and Nano-rheology of Red Blood Cells: an AFM Investigation." Colloids and Surfaces. B, Biointerfaces, vol. 50, no. 1, 2006, pp. 43-8.
Bremmell KE, Evans A, Prestidge CA. Deformation and nano-rheology of red blood cells: an AFM investigation. Colloids Surf B Biointerfaces. 2006;50(1):43-8.
Bremmell, K. E., Evans, A., & Prestidge, C. A. (2006). Deformation and nano-rheology of red blood cells: an AFM investigation. Colloids and Surfaces. B, Biointerfaces, 50(1), pp. 43-8.
Bremmell KE, Evans A, Prestidge CA. Deformation and Nano-rheology of Red Blood Cells: an AFM Investigation. Colloids Surf B Biointerfaces. 2006 Jun 1;50(1):43-8. PubMed PMID: 16701986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deformation and nano-rheology of red blood cells: an AFM investigation. AU - Bremmell,Kristen E, AU - Evans,Allan, AU - Prestidge,Clive A, Y1 - 2006/05/15/ PY - 2005/09/29/received PY - 2006/03/03/revised PY - 2006/03/06/accepted PY - 2006/5/17/pubmed PY - 2006/9/29/medline PY - 2006/5/17/entrez SP - 43 EP - 8 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 50 IS - 1 N2 - Interaction forces, deformation and nano-rheology of individual red blood cells in physiologically relevant solution conditions have been determined by colloid probe atomic force microscopy (AFM). On approach of the physically immobilised cell and silica glass spherical probe surfaces, deformation of the red blood cell was observed in the force curves. At low levels of deformation, spring constants were determined in the range 3-6 m Nm(-1), whereas for higher levels of deformation, the forces increase non-linearly and on retraction, significant force curve hysteresis is observed (i.e. lower forces upon retraction). The extent of force curve hysteresis was dependent on both the drive velocity and loading force, typical of a viscoelastic system. The response of the red blood cell has been described by viscoelastic theory, where the short and long time scale elastic moduli and relaxation times are determined, i.e. the cell's nano-rheological properties elucidated. In addition to a time independent elastic modulus of 4.0 x 10(3)Nm(-2) at low levels of deformation, time-dependent elastic moduli ranges are observed (3.5 x 10(4) to 5.5 x 10(4)Nm(-2) at intermediate levels of deformation and 1.5 x 10(5) to 3.0 x 10(5)Nm(-2) at higher levels of deformation). That is, one elastic and more than one viscoelastic response to the red blood cell deformation is evident, which is considered to reflect the cellular structure. SN - 0927-7765 UR - https://www.unboundmedicine.com/medline/citation/16701986/Deformation_and_nano_rheology_of_red_blood_cells:_an_AFM_investigation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(06)00077-4 DB - PRIME DP - Unbound Medicine ER -