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Stacking, derivatization, and separation by capillary electrophoresis of amino acids from cerebrospinal fluids.
Electrophoresis. 2006 May; 27(10):1922-31.E

Abstract

This paper describes the in-column derivatization, stacking, and separation of amino acids by CE in conjunction with light-emitting diode-induced fluorescence using naphthalene-2,3-dicarboxaldehyde (NDA). According to the relative electrophoretic mobilities and the migration direction in tetraborate solution (pH 9.3), the injection order is cyanide, then amino acids, then NDA. Once poly(ethylene oxide) (PEO) migrates through the capillary under EOF, the amino acid.NDA derivatives, amino acids, and CN- ions migrating against the EOF enter the PEO zone. As a result of increases in viscosity and possible interactions with PEO molecules, the reagents/analytes slow down such that they become stacked at the boundary. In comparison with the off-column approach to the analysis of amino acids, our proposed method provides a lower degree of interference from polymeric NDA compounds and other side products. As a result, the plot of the peak height as a function of gamma-aminobutyric acid (GABA) concentration is linear over the range from 10(-5) to 10(-8) M, with the LOD being 4 nM. We demonstrate the diagnostic potential of this approach for the determination of amino acids, including GABA and glutamine, in biological samples through the analysis of large volumes of cerebral spinal fluids without the need for sample pretreatment.

Authors+Show Affiliations

Chang PL
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Chiu TC
No affiliation info available
Chang HT
No affiliation info available

MeSH

Amino AcidsElectrophoresis, CapillaryFluorometryHumansNaphthalenesPolyethylene GlycolsSolutionsTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16703625

Citation

Chang, Po-Ling, et al. "Stacking, Derivatization, and Separation By Capillary Electrophoresis of Amino Acids From Cerebrospinal Fluids." Electrophoresis, vol. 27, no. 10, 2006, pp. 1922-31.
Chang PL, Chiu TC, Chang HT. Stacking, derivatization, and separation by capillary electrophoresis of amino acids from cerebrospinal fluids. Electrophoresis. 2006;27(10):1922-31.
Chang, P. L., Chiu, T. C., & Chang, H. T. (2006). Stacking, derivatization, and separation by capillary electrophoresis of amino acids from cerebrospinal fluids. Electrophoresis, 27(10), 1922-31.
Chang PL, Chiu TC, Chang HT. Stacking, Derivatization, and Separation By Capillary Electrophoresis of Amino Acids From Cerebrospinal Fluids. Electrophoresis. 2006;27(10):1922-31. PubMed PMID: 16703625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stacking, derivatization, and separation by capillary electrophoresis of amino acids from cerebrospinal fluids. AU - Chang,Po-Ling, AU - Chiu,Tai-Chia, AU - Chang,Huan-Tsung, PY - 2006/5/17/pubmed PY - 2006/8/12/medline PY - 2006/5/17/entrez SP - 1922 EP - 31 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 10 N2 - This paper describes the in-column derivatization, stacking, and separation of amino acids by CE in conjunction with light-emitting diode-induced fluorescence using naphthalene-2,3-dicarboxaldehyde (NDA). According to the relative electrophoretic mobilities and the migration direction in tetraborate solution (pH 9.3), the injection order is cyanide, then amino acids, then NDA. Once poly(ethylene oxide) (PEO) migrates through the capillary under EOF, the amino acid.NDA derivatives, amino acids, and CN- ions migrating against the EOF enter the PEO zone. As a result of increases in viscosity and possible interactions with PEO molecules, the reagents/analytes slow down such that they become stacked at the boundary. In comparison with the off-column approach to the analysis of amino acids, our proposed method provides a lower degree of interference from polymeric NDA compounds and other side products. As a result, the plot of the peak height as a function of gamma-aminobutyric acid (GABA) concentration is linear over the range from 10(-5) to 10(-8) M, with the LOD being 4 nM. We demonstrate the diagnostic potential of this approach for the determination of amino acids, including GABA and glutamine, in biological samples through the analysis of large volumes of cerebral spinal fluids without the need for sample pretreatment. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16703625/Stacking_derivatization_and_separation_by_capillary_electrophoresis_of_amino_acids_from_cerebrospinal_fluids_ DB - PRIME DP - Unbound Medicine ER -