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[Changes of nerve growth factor and its receptors in the lung tissues in asthmatic rats and their effects on the airway inflammation].
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Dec; 30(6):660-5.ZN

Abstract

OBJECTIVE

To determine the expression of nerve growth factor (NGF), tyrosine kinase receptor A (trkA), and pan-neurotrophin receptor (p75) in the lung tissues in asthmatic rats, and to explore their effects on the airway inflammation.

METHODS

Thirty-two SD rats were randomly divided into 4 groups: the control, asthma, NGF and anti-NGF groups. The asthmatic model was established by the inhalation and injection of ovalbumin. The total cell count and differential cell count in the bronchoalveolar lavage fluid (BALF) were performed. The pathologic changes in the lung tissues of the 4 groups was detected by HE staining. The NGF mRNA expression in the lung tissues of the asthma and control groups was determined by reverse transcription-polymerase chain reaction (RT-PCR). The changes of trkA and p75 mRNA expressions in the lung tissues in the 4 groups were also investigated by RT-PCR.

RESULTS

Compared with the control group, the BALF total cell, the BALF eosinophils (Eos), and the BALF lymphocytes (Lyms) significantly increased (All P <0. 001) in the asthma group; and the lung tissues of the asthma group had more infiltrating inflammatory cells. Not only the expression of NGF mRNA, but also its receptors trkA and p75 mRNA in the lung tissues were significantly higher in the asthma group than those in the control group (All P < 0.01). Positive correlation was found between the expression of NGF mRNA and the BALF total cell, the BALF Lyms in the asthma group. Compared with the asthma group, the total cell, the Eos, and the lyms in BALF in the NGF group significantly increased (All P < 0.01), and the lungs of the NGF group had apparent inflammatory changes. The expre-ssions of p75 and trkA mRNA were enhanced significantly (All P < 0.05). Compared with the asthma group, the total cell, the Eos, and the lyms in BALF in the anti-NGF group significantly decreased (All P < 0.001), and the lungs of the anti-NGF group showed alleviative inflammatory changes. The expre-ssions of p75 and trkA mRNA significantly decreased (All P < 0.01).

CONCLUSION

In lungs of asthmatic rats, the elevated expression of NGF mRNA is closely related to the airway inflammation. NGF can upregulate the expressions of p75 and trkA mRNA in asthmatic rats, and then may promote their role in the airway neuronal inflammation in asthma.

Authors+Show Affiliations

Ouyang RY
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Hu CP
No affiliation info available
Zhu JQ
No affiliation info available
Huang XG
No affiliation info available

MeSH

AnimalsAsthmaBronchitisLungMaleNerve Growth FactorOvalbuminRNA, MessengerRandom AllocationRatsRats, Sprague-DawleyReceptor, trkAReceptors, Nerve Growth Factor

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16708804

Citation

Ouyang, Ruo-yun, et al. "[Changes of Nerve Growth Factor and Its Receptors in the Lung Tissues in Asthmatic Rats and Their Effects On the Airway Inflammation]." Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, vol. 30, no. 6, 2005, pp. 660-5.
Ouyang RY, Hu CP, Zhu JQ, et al. [Changes of nerve growth factor and its receptors in the lung tissues in asthmatic rats and their effects on the airway inflammation]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005;30(6):660-5.
Ouyang, R. Y., Hu, C. P., Zhu, J. Q., & Huang, X. G. (2005). [Changes of nerve growth factor and its receptors in the lung tissues in asthmatic rats and their effects on the airway inflammation]. Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, 30(6), 660-5.
Ouyang RY, et al. [Changes of Nerve Growth Factor and Its Receptors in the Lung Tissues in Asthmatic Rats and Their Effects On the Airway Inflammation]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005;30(6):660-5. PubMed PMID: 16708804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Changes of nerve growth factor and its receptors in the lung tissues in asthmatic rats and their effects on the airway inflammation]. AU - Ouyang,Ruo-yun, AU - Hu,Cheng-ping, AU - Zhu,Jin-qi, AU - Huang,Xin-gang, PY - 2006/5/20/pubmed PY - 2006/9/13/medline PY - 2006/5/20/entrez SP - 660 EP - 5 JF - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences JO - Zhong Nan Da Xue Xue Bao Yi Xue Ban VL - 30 IS - 6 N2 - OBJECTIVE: To determine the expression of nerve growth factor (NGF), tyrosine kinase receptor A (trkA), and pan-neurotrophin receptor (p75) in the lung tissues in asthmatic rats, and to explore their effects on the airway inflammation. METHODS: Thirty-two SD rats were randomly divided into 4 groups: the control, asthma, NGF and anti-NGF groups. The asthmatic model was established by the inhalation and injection of ovalbumin. The total cell count and differential cell count in the bronchoalveolar lavage fluid (BALF) were performed. The pathologic changes in the lung tissues of the 4 groups was detected by HE staining. The NGF mRNA expression in the lung tissues of the asthma and control groups was determined by reverse transcription-polymerase chain reaction (RT-PCR). The changes of trkA and p75 mRNA expressions in the lung tissues in the 4 groups were also investigated by RT-PCR. RESULTS: Compared with the control group, the BALF total cell, the BALF eosinophils (Eos), and the BALF lymphocytes (Lyms) significantly increased (All P <0. 001) in the asthma group; and the lung tissues of the asthma group had more infiltrating inflammatory cells. Not only the expression of NGF mRNA, but also its receptors trkA and p75 mRNA in the lung tissues were significantly higher in the asthma group than those in the control group (All P < 0.01). Positive correlation was found between the expression of NGF mRNA and the BALF total cell, the BALF Lyms in the asthma group. Compared with the asthma group, the total cell, the Eos, and the lyms in BALF in the NGF group significantly increased (All P < 0.01), and the lungs of the NGF group had apparent inflammatory changes. The expre-ssions of p75 and trkA mRNA were enhanced significantly (All P < 0.05). Compared with the asthma group, the total cell, the Eos, and the lyms in BALF in the anti-NGF group significantly decreased (All P < 0.001), and the lungs of the anti-NGF group showed alleviative inflammatory changes. The expre-ssions of p75 and trkA mRNA significantly decreased (All P < 0.01). CONCLUSION: In lungs of asthmatic rats, the elevated expression of NGF mRNA is closely related to the airway inflammation. NGF can upregulate the expressions of p75 and trkA mRNA in asthmatic rats, and then may promote their role in the airway neuronal inflammation in asthma. SN - 1672-7347 UR - https://www.unboundmedicine.com/medline/citation/16708804/[Changes_of_nerve_growth_factor_and_its_receptors_in_the_lung_tissues_in_asthmatic_rats_and_their_effects_on_the_airway_inflammation]_ DB - PRIME DP - Unbound Medicine ER -