Tags

Type your tag names separated by a space and hit enter

AhSSK1, a novel SKP1-like protein that interacts with the S-locus F-box protein SLF.
Plant J. 2006 Jun; 46(5):780-93.PJ

Abstract

The S-locus F-box (SLF/SFB) protein, recently identified as the pollen determinant of S-RNase-based self-incompatibility (SI) in Solanaceae, Scrophulariaceae and Rosaceae, has been proposed to serve as the subunit of an SCF (SKP1-CUL1-F-box) ubiquitin ligase and to target its pistil counterpart S-RNase during the SI response. However, the underlying mechanism is still in dispute, and the putative SLF-binding SKP1-equivalent protein remains unknown. Here, we report the identification of AhSSK1, Antirrhinum hispanicumSLF-interacting SKP1-like1, using a yeast two-hybrid screen against a pollen cDNA library. GST pull-down assays confirmed the SSK1-SLF interaction, and showed that AhSSK1 could connect AhSLF to a CUL1-like protein. AhSSK1, despite having a similar secondary structure to other SKP1-like proteins, appeared quite distinctive in sequence and unique in a phylogenetic analysis, in which no SSK1 ortholog could be predicted in the sequenced genomes of Arabidopsis and rice. Thus, our results suggest that the pollen-specific SSK1 could be recruited exclusively as the adaptor of putative SCF(SLF) in those plants with S-RNase-based SI, providing an important clue to dissecting the function of the pollen determinant.

Authors+Show Affiliations

Huang J
Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, Beijing 100080, China.
Zhao L
No affiliation info available
Yang Q
No affiliation info available
Xue Y
No affiliation info available

MeSH

Alternative SplicingAmino Acid MotifsAmino Acid SequenceAntirrhinumF-Box ProteinsGenome, PlantMolecular Sequence DataPhylogenyPlant ProteinsPollenProtein Interaction MappingS-Phase Kinase-Associated ProteinsSequence AlignmentSequence Analysis, ProteinTwo-Hybrid System Techniques

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16709194

Citation

Huang, Jian, et al. "AhSSK1, a Novel SKP1-like Protein That Interacts With the S-locus F-box Protein SLF." The Plant Journal : for Cell and Molecular Biology, vol. 46, no. 5, 2006, pp. 780-93.
Huang J, Zhao L, Yang Q, et al. AhSSK1, a novel SKP1-like protein that interacts with the S-locus F-box protein SLF. Plant J. 2006;46(5):780-93.
Huang, J., Zhao, L., Yang, Q., & Xue, Y. (2006). AhSSK1, a novel SKP1-like protein that interacts with the S-locus F-box protein SLF. The Plant Journal : for Cell and Molecular Biology, 46(5), 780-93.
Huang J, et al. AhSSK1, a Novel SKP1-like Protein That Interacts With the S-locus F-box Protein SLF. Plant J. 2006;46(5):780-93. PubMed PMID: 16709194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - AhSSK1, a novel SKP1-like protein that interacts with the S-locus F-box protein SLF. AU - Huang,Jian, AU - Zhao,Lan, AU - Yang,Qiuying, AU - Xue,Yongbiao, PY - 2006/5/20/pubmed PY - 2006/8/5/medline PY - 2006/5/20/entrez SP - 780 EP - 93 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 46 IS - 5 N2 - The S-locus F-box (SLF/SFB) protein, recently identified as the pollen determinant of S-RNase-based self-incompatibility (SI) in Solanaceae, Scrophulariaceae and Rosaceae, has been proposed to serve as the subunit of an SCF (SKP1-CUL1-F-box) ubiquitin ligase and to target its pistil counterpart S-RNase during the SI response. However, the underlying mechanism is still in dispute, and the putative SLF-binding SKP1-equivalent protein remains unknown. Here, we report the identification of AhSSK1, Antirrhinum hispanicumSLF-interacting SKP1-like1, using a yeast two-hybrid screen against a pollen cDNA library. GST pull-down assays confirmed the SSK1-SLF interaction, and showed that AhSSK1 could connect AhSLF to a CUL1-like protein. AhSSK1, despite having a similar secondary structure to other SKP1-like proteins, appeared quite distinctive in sequence and unique in a phylogenetic analysis, in which no SSK1 ortholog could be predicted in the sequenced genomes of Arabidopsis and rice. Thus, our results suggest that the pollen-specific SSK1 could be recruited exclusively as the adaptor of putative SCF(SLF) in those plants with S-RNase-based SI, providing an important clue to dissecting the function of the pollen determinant. SN - 0960-7412 UR - https://www.unboundmedicine.com/medline/citation/16709194/AhSSK1_a_novel_SKP1_like_protein_that_interacts_with_the_S_locus_F_box_protein_SLF_ DB - PRIME DP - Unbound Medicine ER -