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[Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC].
Zhongguo Zhong Yao Za Zhi. 2006 Mar; 31(5):386-8.ZZ

Abstract

OBJECTIVE

To determine 20(S)-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium.

METHOD

The separation was performed on ZORBAX EXEND C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water (85:15) as mobile phase with the rate of 1.2 mL x min(-1) at 25 degrees C, the wavelength for measurement was 203 nm.

RESULT

The calibration curve was linear in the range of 0.5-25 microg for 20(S)-ginsengnoside Rh2(r = 0.9999, n = 7). The average recovery was 99.7% (RSD= 1.0%).

CONCLUSION

This method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20(S)-ginsengnoside Rh2 is high by this hydrolysis method.

Authors+Show Affiliations

Li XW
College of Chemistry, Jilin Unirersity, Changchun, China. xwli@jlu.edu.cn
Gui MY
No affiliation info available
Zheng Y
No affiliation info available
Jin YR
No affiliation info available
Zhang HQ
No affiliation info available

MeSH

Chromatography, High Pressure LiquidGinsenosidesHydrolysisPanaxPlant LeavesPlant StemsPlants, Medicinal

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16711421

Citation

Li, Xu-wen, et al. "[Determination of 20 (S)-ginsengnoside Rh2 in the Alkali-hydrolysis Product of Saponins From Leaves of Panax Qinquefolium By RP-HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 31, no. 5, 2006, pp. 386-8.
Li XW, Gui MY, Zheng Y, et al. [Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2006;31(5):386-8.
Li, X. W., Gui, M. Y., Zheng, Y., Jin, Y. R., & Zhang, H. Q. (2006). [Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 31(5), 386-8.
Li XW, et al. [Determination of 20 (S)-ginsengnoside Rh2 in the Alkali-hydrolysis Product of Saponins From Leaves of Panax Qinquefolium By RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2006;31(5):386-8. PubMed PMID: 16711421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC]. AU - Li,Xu-wen, AU - Gui,Ming-yu, AU - Zheng,Ying, AU - Jin,Yong-ri, AU - Zhang,Han-qi, PY - 2006/5/23/pubmed PY - 2006/10/7/medline PY - 2006/5/23/entrez SP - 386 EP - 8 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 31 IS - 5 N2 - OBJECTIVE: To determine 20(S)-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium. METHOD: The separation was performed on ZORBAX EXEND C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water (85:15) as mobile phase with the rate of 1.2 mL x min(-1) at 25 degrees C, the wavelength for measurement was 203 nm. RESULT: The calibration curve was linear in the range of 0.5-25 microg for 20(S)-ginsengnoside Rh2(r = 0.9999, n = 7). The average recovery was 99.7% (RSD= 1.0%). CONCLUSION: This method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20(S)-ginsengnoside Rh2 is high by this hydrolysis method. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/16711421/[Determination_of_20__S__ginsengnoside_Rh2_in_the_alkali_hydrolysis_product_of_saponins_from_leaves_of_Panax_qinquefolium_by_RP_HPLC]_ DB - PRIME DP - Unbound Medicine ER -