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[Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC].
Zhongguo Zhong Yao Za Zhi. 2006 Mar; 31(5):386-8.ZZ

Abstract

OBJECTIVE

To determine 20(S)-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium.

METHOD

The separation was performed on ZORBAX EXEND C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water (85:15) as mobile phase with the rate of 1.2 mL x min(-1) at 25 degrees C, the wavelength for measurement was 203 nm.

RESULT

The calibration curve was linear in the range of 0.5-25 microg for 20(S)-ginsengnoside Rh2(r = 0.9999, n = 7). The average recovery was 99.7% (RSD= 1.0%).

CONCLUSION

This method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20(S)-ginsengnoside Rh2 is high by this hydrolysis method.

Authors+Show Affiliations

College of Chemistry, Jilin Unirersity, Changchun, China. xwli@jlu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

16711421

Citation

Li, Xu-wen, et al. "[Determination of 20 (S)-ginsengnoside Rh2 in the Alkali-hydrolysis Product of Saponins From Leaves of Panax Qinquefolium By RP-HPLC]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 31, no. 5, 2006, pp. 386-8.
Li XW, Gui MY, Zheng Y, et al. [Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2006;31(5):386-8.
Li, X. W., Gui, M. Y., Zheng, Y., Jin, Y. R., & Zhang, H. Q. (2006). [Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 31(5), 386-8.
Li XW, et al. [Determination of 20 (S)-ginsengnoside Rh2 in the Alkali-hydrolysis Product of Saponins From Leaves of Panax Qinquefolium By RP-HPLC]. Zhongguo Zhong Yao Za Zhi. 2006;31(5):386-8. PubMed PMID: 16711421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of 20 (S)-ginsengnoside Rh2 in the alkali-hydrolysis product of saponins from leaves of Panax qinquefolium by RP-HPLC]. AU - Li,Xu-wen, AU - Gui,Ming-yu, AU - Zheng,Ying, AU - Jin,Yong-ri, AU - Zhang,Han-qi, PY - 2006/5/23/pubmed PY - 2006/10/7/medline PY - 2006/5/23/entrez SP - 386 EP - 8 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 31 IS - 5 N2 - OBJECTIVE: To determine 20(S)-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium. METHOD: The separation was performed on ZORBAX EXEND C18 column (4.6 mm x 250 mm, 5 microm), eluted with methanol and water (85:15) as mobile phase with the rate of 1.2 mL x min(-1) at 25 degrees C, the wavelength for measurement was 203 nm. RESULT: The calibration curve was linear in the range of 0.5-25 microg for 20(S)-ginsengnoside Rh2(r = 0.9999, n = 7). The average recovery was 99.7% (RSD= 1.0%). CONCLUSION: This method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20(S)-ginsengnoside Rh2 is high by this hydrolysis method. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/16711421/[Determination_of_20__S__ginsengnoside_Rh2_in_the_alkali_hydrolysis_product_of_saponins_from_leaves_of_Panax_qinquefolium_by_RP_HPLC]_ DB - PRIME DP - Unbound Medicine ER -