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Changes in liver PPARalpha mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice.
J Nutr Biochem. 2007 Feb; 18(2):86-96.JN

Abstract

The ligand-dependent transcription factor peroxisome proliferator-activated receptor alpha (PPARalpha) is known to be activated by common fatty acids and to regulate the expression of genes of various lipid oxidation pathways and transport. High-fat diets provide more fatty acids, which presumably could enhance lipid catabolism through up-regulation of PPARalpha signaling. However, high intake of fat could also lead to obesity. To examine PPARalpha signaling in high-fat feeding and obesity, this study examined the hepatic mRNA expression of PPARalpha and some of its target genes in Wistar rats and C57BL/6J mice fed two levels (20% or 30% wt/wt) of high-safflower-oil (SFO; oleic-acid-rich) diets until animals showed significantly higher body weight (13 weeks for rats and 22 weeks for mice) than those of control groups fed a 5% SFO diet. At the end of these respective feeding periods, only the rats fed 30% SFO and the mice fed 20% SFO among the two groups fed high-fat diets showed significantly higher body weight, white adipose tissue weight, serum leptin and mRNA expression of PPARalpha (P<.05) compared to the respective control groups. Despite elevated acyl-CoA (a PPARalpha target gene) protein and activity in both groups fed high-fat diets, the mRNA expression level of most PPARalpha target genes examined correlated mainly to PPARalpha mRNA levels and not to fat intake or liver lipid levels. The observation that the liver PPARalpha mRNA expression in groups fed high-fat diets was significantly higher only in obese animals with elevated serum leptin implied that obesity and associated hyperleptinemia might have a stronger impact than dietary SFO intake per se on PPARalpha-regulated mRNA expression in the liver.

Authors+Show Affiliations

Division of Nutritional Science, Institute of Microbiology and Biochemistry, College of Life Science, National Taiwan University, Taipei 106, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16713235

Citation

Hsu, Shan-Ching, and Ching-jang Huang. "Changes in Liver PPARalpha mRNA Expression in Response to Two Levels of High-safflower-oil Diets Correlate With Changes in Adiposity and Serum Leptin in Rats and Mice." The Journal of Nutritional Biochemistry, vol. 18, no. 2, 2007, pp. 86-96.
Hsu SC, Huang CJ. Changes in liver PPARalpha mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice. J Nutr Biochem. 2007;18(2):86-96.
Hsu, S. C., & Huang, C. J. (2007). Changes in liver PPARalpha mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice. The Journal of Nutritional Biochemistry, 18(2), 86-96.
Hsu SC, Huang CJ. Changes in Liver PPARalpha mRNA Expression in Response to Two Levels of High-safflower-oil Diets Correlate With Changes in Adiposity and Serum Leptin in Rats and Mice. J Nutr Biochem. 2007;18(2):86-96. PubMed PMID: 16713235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in liver PPARalpha mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice. AU - Hsu,Shan-Ching, AU - Huang,Ching-jang, Y1 - 2006/05/18/ PY - 2005/12/23/received PY - 2006/02/18/revised PY - 2006/03/02/accepted PY - 2006/5/23/pubmed PY - 2007/3/9/medline PY - 2006/5/23/entrez SP - 86 EP - 96 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 18 IS - 2 N2 - The ligand-dependent transcription factor peroxisome proliferator-activated receptor alpha (PPARalpha) is known to be activated by common fatty acids and to regulate the expression of genes of various lipid oxidation pathways and transport. High-fat diets provide more fatty acids, which presumably could enhance lipid catabolism through up-regulation of PPARalpha signaling. However, high intake of fat could also lead to obesity. To examine PPARalpha signaling in high-fat feeding and obesity, this study examined the hepatic mRNA expression of PPARalpha and some of its target genes in Wistar rats and C57BL/6J mice fed two levels (20% or 30% wt/wt) of high-safflower-oil (SFO; oleic-acid-rich) diets until animals showed significantly higher body weight (13 weeks for rats and 22 weeks for mice) than those of control groups fed a 5% SFO diet. At the end of these respective feeding periods, only the rats fed 30% SFO and the mice fed 20% SFO among the two groups fed high-fat diets showed significantly higher body weight, white adipose tissue weight, serum leptin and mRNA expression of PPARalpha (P<.05) compared to the respective control groups. Despite elevated acyl-CoA (a PPARalpha target gene) protein and activity in both groups fed high-fat diets, the mRNA expression level of most PPARalpha target genes examined correlated mainly to PPARalpha mRNA levels and not to fat intake or liver lipid levels. The observation that the liver PPARalpha mRNA expression in groups fed high-fat diets was significantly higher only in obese animals with elevated serum leptin implied that obesity and associated hyperleptinemia might have a stronger impact than dietary SFO intake per se on PPARalpha-regulated mRNA expression in the liver. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/16713235/Changes_in_liver_PPARalpha_mRNA_expression_in_response_to_two_levels_of_high_safflower_oil_diets_correlate_with_changes_in_adiposity_and_serum_leptin_in_rats_and_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(06)00070-2 DB - PRIME DP - Unbound Medicine ER -