Tags

Type your tag names separated by a space and hit enter

7-Ketocholesterol enhances 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells.
J Neural Transm (Vienna). 2006 Dec; 113(12):1877-85.JN

Abstract

The present study investigated the promoting effect of oxysterol 7-ketocholesterol against the cytotoxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in differentiated PC12 cells. 7-Ketocholesterol significantly enhanced the MPP(+)-induced nuclear damage, decrease in the mitochondrial transmembrane potential, cytosolic accumulation of cytochrome c, activation of caspase-3, increase in the formation of reactive oxygen species and depletion of GSH. N-Acetylcysteine, ascorbate, trolox, carboxy-PTIO and Mn-TBAP reduced the cytotoxic effect of MPP(+) in the presence of 7-ketocholesterol. The results indicate that 7-ketocholesterol shows a synergistic effect against the cytotoxic effect of MPP(+). 7-Ketocholesterol may enhance the MPP(+)-induced viability loss in PC12 cells by promoting the mitochondrial membrane permeability change, release of cytochrome c and subsequent activation of caspase-3, which is associated with the increased formation of reactive oxygen species and depletion of GSH. The findings suggest that 7-ketocholesterol as a promoting agent for the formation of mitochondrial permeability transition may enhance the toxic neuronal cell injury.

Authors+Show Affiliations

Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16715209

Citation

Kim, Y J., et al. "7-Ketocholesterol Enhances 1-methyl-4-phenylpyridinium-induced Mitochondrial Dysfunction and Cell Death in PC12 Cells." Journal of Neural Transmission (Vienna, Austria : 1996), vol. 113, no. 12, 2006, pp. 1877-85.
Kim YJ, Han JH, Han ES, et al. 7-Ketocholesterol enhances 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells. J Neural Transm (Vienna). 2006;113(12):1877-85.
Kim, Y. J., Han, J. H., Han, E. S., & Lee, C. S. (2006). 7-Ketocholesterol enhances 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells. Journal of Neural Transmission (Vienna, Austria : 1996), 113(12), 1877-85.
Kim YJ, et al. 7-Ketocholesterol Enhances 1-methyl-4-phenylpyridinium-induced Mitochondrial Dysfunction and Cell Death in PC12 Cells. J Neural Transm (Vienna). 2006;113(12):1877-85. PubMed PMID: 16715209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 7-Ketocholesterol enhances 1-methyl-4-phenylpyridinium-induced mitochondrial dysfunction and cell death in PC12 cells. AU - Kim,Y J, AU - Han,J H, AU - Han,E S, AU - Lee,C S, Y1 - 2006/05/24/ PY - 2005/10/10/received PY - 2006/03/13/accepted PY - 2006/5/23/pubmed PY - 2007/10/18/medline PY - 2006/5/23/entrez SP - 1877 EP - 85 JF - Journal of neural transmission (Vienna, Austria : 1996) JO - J Neural Transm (Vienna) VL - 113 IS - 12 N2 - The present study investigated the promoting effect of oxysterol 7-ketocholesterol against the cytotoxicity of 1-methyl-4-phenylpyridinium (MPP(+)) in differentiated PC12 cells. 7-Ketocholesterol significantly enhanced the MPP(+)-induced nuclear damage, decrease in the mitochondrial transmembrane potential, cytosolic accumulation of cytochrome c, activation of caspase-3, increase in the formation of reactive oxygen species and depletion of GSH. N-Acetylcysteine, ascorbate, trolox, carboxy-PTIO and Mn-TBAP reduced the cytotoxic effect of MPP(+) in the presence of 7-ketocholesterol. The results indicate that 7-ketocholesterol shows a synergistic effect against the cytotoxic effect of MPP(+). 7-Ketocholesterol may enhance the MPP(+)-induced viability loss in PC12 cells by promoting the mitochondrial membrane permeability change, release of cytochrome c and subsequent activation of caspase-3, which is associated with the increased formation of reactive oxygen species and depletion of GSH. The findings suggest that 7-ketocholesterol as a promoting agent for the formation of mitochondrial permeability transition may enhance the toxic neuronal cell injury. SN - 0300-9564 UR - https://www.unboundmedicine.com/medline/citation/16715209/7_Ketocholesterol_enhances_1_methyl_4_phenylpyridinium_induced_mitochondrial_dysfunction_and_cell_death_in_PC12_cells_ L2 - https://doi.org/10.1007/s00702-006-0486-6 DB - PRIME DP - Unbound Medicine ER -