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Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii lambda-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis.
Carbohydr Res. 2006 Aug 14; 341(11):1859-69.CR

Abstract

lambda-Carrageenan extracted from Gigartina skottsbergii tetrasporophyte was completely digested by a purified Pseudoalteromonas carrageenovora lambda-carrageenase. The main digestion products were fractionated and analysed by (1)H and (13)C NMR spectroscopy. All the oligosaccharides observed belong to the neo-carrabiose oligosaccharide series indicating that the lambda-carrageenase cleaves the beta-(1-->4) glycosidic bonds. (1)H and (13)C NMR spectra recorded on oligomers from DP 2 to DP 8 were fully interpreted allowing unambiguous assignment of the lambda-carrageenan spectra. Besides the typical oligo-lambda-carrageenans, we have also characterised a heptasulfated tetrasaccharide which demonstrates the random over-sulfation along the chain of G. skottsbergii lambda-carrageenan.

Authors+Show Affiliations

Végétaux Marins et Biomolécules, UMR 7139 (CNRS/UPMC), Station Biologique, Roscoff, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16716278

Citation

Guibet, Marion, et al. "Complete Assignment of 1H and 13C NMR Spectra of Gigartina Skottsbergii Lambda-carrageenan Using Carrabiose Oligosaccharides Prepared By Enzymatic Hydrolysis." Carbohydrate Research, vol. 341, no. 11, 2006, pp. 1859-69.
Guibet M, Kervarec N, Génicot S, et al. Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii lambda-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis. Carbohydr Res. 2006;341(11):1859-69.
Guibet, M., Kervarec, N., Génicot, S., Chevolot, Y., & Helbert, W. (2006). Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii lambda-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis. Carbohydrate Research, 341(11), 1859-69.
Guibet M, et al. Complete Assignment of 1H and 13C NMR Spectra of Gigartina Skottsbergii Lambda-carrageenan Using Carrabiose Oligosaccharides Prepared By Enzymatic Hydrolysis. Carbohydr Res. 2006 Aug 14;341(11):1859-69. PubMed PMID: 16716278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complete assignment of 1H and 13C NMR spectra of Gigartina skottsbergii lambda-carrageenan using carrabiose oligosaccharides prepared by enzymatic hydrolysis. AU - Guibet,Marion, AU - Kervarec,Nelly, AU - Génicot,Sabine, AU - Chevolot,Yann, AU - Helbert,William, Y1 - 2006/05/23/ PY - 2006/02/01/received PY - 2006/04/14/revised PY - 2006/04/18/accepted PY - 2006/5/24/pubmed PY - 2006/9/26/medline PY - 2006/5/24/entrez SP - 1859 EP - 69 JF - Carbohydrate research JO - Carbohydr Res VL - 341 IS - 11 N2 - lambda-Carrageenan extracted from Gigartina skottsbergii tetrasporophyte was completely digested by a purified Pseudoalteromonas carrageenovora lambda-carrageenase. The main digestion products were fractionated and analysed by (1)H and (13)C NMR spectroscopy. All the oligosaccharides observed belong to the neo-carrabiose oligosaccharide series indicating that the lambda-carrageenase cleaves the beta-(1-->4) glycosidic bonds. (1)H and (13)C NMR spectra recorded on oligomers from DP 2 to DP 8 were fully interpreted allowing unambiguous assignment of the lambda-carrageenan spectra. Besides the typical oligo-lambda-carrageenans, we have also characterised a heptasulfated tetrasaccharide which demonstrates the random over-sulfation along the chain of G. skottsbergii lambda-carrageenan. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/16716278/Complete_assignment_of_1H_and_13C_NMR_spectra_of_Gigartina_skottsbergii_lambda_carrageenan_using_carrabiose_oligosaccharides_prepared_by_enzymatic_hydrolysis_ DB - PRIME DP - Unbound Medicine ER -