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Development and validation of a capillary electrophoresis method with laser-induced fluorescence detection for the determination of captopril in human urine and pharmaceutical preparations.
Electrophoresis. 2006 Jun; 27(12):2310-6.E

Abstract

This study describes the development of a CE method for the analysis of the antihypertensive drug captopril using LIF detection. The method is based on the derivatization of captopril with the fluorescent label 5-iodoacetamidofluorescein. The optimization of the electrophoretic electrolyte composition together with other variables, such as applied voltage and injection time, resulted in a solution of 20 mM phosphate buffer adjusted to pH 12.0. The calibration curve for the fluorescent captopril derivative was linear in the concentration range 3.5-6000 ng/mL with a detection limit of 0.5 ng/mL. Intra- and interday precision (at a concentration of about 100 times the LOD) were less than 0.86 and 1.16%, respectively, both expressed as RSD. The assay was successfully used for quantification of captopril in some marketed pharmaceutical preparations and urine samples.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Murcia, Spain. tpr@um.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

16718646

Citation

Pérez-Ruiz, Tomás, et al. "Development and Validation of a Capillary Electrophoresis Method With Laser-induced Fluorescence Detection for the Determination of Captopril in Human Urine and Pharmaceutical Preparations." Electrophoresis, vol. 27, no. 12, 2006, pp. 2310-6.
Pérez-Ruiz T, Martínez-Lozano C, Galera R. Development and validation of a capillary electrophoresis method with laser-induced fluorescence detection for the determination of captopril in human urine and pharmaceutical preparations. Electrophoresis. 2006;27(12):2310-6.
Pérez-Ruiz, T., Martínez-Lozano, C., & Galera, R. (2006). Development and validation of a capillary electrophoresis method with laser-induced fluorescence detection for the determination of captopril in human urine and pharmaceutical preparations. Electrophoresis, 27(12), 2310-6.
Pérez-Ruiz T, Martínez-Lozano C, Galera R. Development and Validation of a Capillary Electrophoresis Method With Laser-induced Fluorescence Detection for the Determination of Captopril in Human Urine and Pharmaceutical Preparations. Electrophoresis. 2006;27(12):2310-6. PubMed PMID: 16718646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a capillary electrophoresis method with laser-induced fluorescence detection for the determination of captopril in human urine and pharmaceutical preparations. AU - Pérez-Ruiz,Tomás, AU - Martínez-Lozano,Carmen, AU - Galera,Raquel, PY - 2006/5/24/pubmed PY - 2006/9/14/medline PY - 2006/5/24/entrez SP - 2310 EP - 6 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 12 N2 - This study describes the development of a CE method for the analysis of the antihypertensive drug captopril using LIF detection. The method is based on the derivatization of captopril with the fluorescent label 5-iodoacetamidofluorescein. The optimization of the electrophoretic electrolyte composition together with other variables, such as applied voltage and injection time, resulted in a solution of 20 mM phosphate buffer adjusted to pH 12.0. The calibration curve for the fluorescent captopril derivative was linear in the concentration range 3.5-6000 ng/mL with a detection limit of 0.5 ng/mL. Intra- and interday precision (at a concentration of about 100 times the LOD) were less than 0.86 and 1.16%, respectively, both expressed as RSD. The assay was successfully used for quantification of captopril in some marketed pharmaceutical preparations and urine samples. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16718646/Development_and_validation_of_a_capillary_electrophoresis_method_with_laser_induced_fluorescence_detection_for_the_determination_of_captopril_in_human_urine_and_pharmaceutical_preparations_ L2 - https://doi.org/10.1002/elps.200500861 DB - PRIME DP - Unbound Medicine ER -