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Application of reverse-flow micellar electrokinetic chromatography for the simultaneous determination of flavonols and terpene trilactones in Ginkgo biloba dosage forms.
J Chromatogr A. 2006 Jul 28; 1122(1-2):266-74.JC

Abstract

A reverse-flow micellar electrokinetic chromatographic (RF-MEKC) method was developed for the simultaneous qualitative determination of 10 components consisting of the flavonol glycosides, rutin and quercitrin, the flavonol aglycones, isorhamnetin, kaempferol and quercetin, the terpene trilactones, ginkgolides A, B, C and J and the sesquiterpene, bilobalide. This method was used to fingerprint Ginkgo biloba solid oral dosage forms and validated for the quantitation of the marker compounds, rutin and quercetin in some commercial products. In addition to the usual variables, the influence of some essential background electrolyte (BGE) components such as sodium dodecyl sulphate (SDS) and <beta>-cyclodextrin concentrations were investigated. A polyimide fused-silica square capillary column (75 microm I.D. x 360 microm O.D.) with a total length of 60.0 cm and effective length of 45.0 cm was used for the separation. The final BGE consisted of 20 mM phosphoric acid, 40 mM SDS and 12 mM <beta>-cyclodextrin (pH 2.2) using reverse polarity with a voltage of -17.5 kV. Samples were injected electrokinetically at -5 kV for 3 s for the qualitative analysis and hydrodynamically at 20 mbar for 0.6 s for the quantitative assay. The total run time was 22 min and the limits of detection were 3.13 microg/ml and 1.88 microg/ml for rutin and quercetin, respectively. Fingerprint profiles of the solid oral dosage forms and the results of the quantitative analysis indicated that there were major discrepancies in the marker content between products and illustrates the value of this method for use as a procedure to assess product quality of commercially available Ginkgo biloba products.

Authors+Show Affiliations

Dubber MJ
Faculty of Pharmacy, Division of Pharmaceutics, Rhodes University, Grahamstown 6139, South Africa.
Kanfer I
No affiliation info available

MeSH

Chromatography, Micellar Electrokinetic CapillaryFlavonolsGinkgo bilobaMolecular StructurePlants, MedicinalPowdersQuality ControlReproducibility of ResultsTabletsTerpenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16720026

Citation

Dubber, M-J, and I Kanfer. "Application of Reverse-flow Micellar Electrokinetic Chromatography for the Simultaneous Determination of Flavonols and Terpene Trilactones in Ginkgo Biloba Dosage Forms." Journal of Chromatography. A, vol. 1122, no. 1-2, 2006, pp. 266-74.
Dubber MJ, Kanfer I. Application of reverse-flow micellar electrokinetic chromatography for the simultaneous determination of flavonols and terpene trilactones in Ginkgo biloba dosage forms. J Chromatogr A. 2006;1122(1-2):266-74.
Dubber, M. J., & Kanfer, I. (2006). Application of reverse-flow micellar electrokinetic chromatography for the simultaneous determination of flavonols and terpene trilactones in Ginkgo biloba dosage forms. Journal of Chromatography. A, 1122(1-2), 266-74.
Dubber MJ, Kanfer I. Application of Reverse-flow Micellar Electrokinetic Chromatography for the Simultaneous Determination of Flavonols and Terpene Trilactones in Ginkgo Biloba Dosage Forms. J Chromatogr A. 2006 Jul 28;1122(1-2):266-74. PubMed PMID: 16720026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of reverse-flow micellar electrokinetic chromatography for the simultaneous determination of flavonols and terpene trilactones in Ginkgo biloba dosage forms. AU - Dubber,M-J, AU - Kanfer,I, Y1 - 2006/05/23/ PY - 2005/11/10/received PY - 2006/04/17/revised PY - 2006/04/24/accepted PY - 2006/5/25/pubmed PY - 2006/9/29/medline PY - 2006/5/25/entrez SP - 266 EP - 74 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1122 IS - 1-2 N2 - A reverse-flow micellar electrokinetic chromatographic (RF-MEKC) method was developed for the simultaneous qualitative determination of 10 components consisting of the flavonol glycosides, rutin and quercitrin, the flavonol aglycones, isorhamnetin, kaempferol and quercetin, the terpene trilactones, ginkgolides A, B, C and J and the sesquiterpene, bilobalide. This method was used to fingerprint Ginkgo biloba solid oral dosage forms and validated for the quantitation of the marker compounds, rutin and quercetin in some commercial products. In addition to the usual variables, the influence of some essential background electrolyte (BGE) components such as sodium dodecyl sulphate (SDS) and <beta>-cyclodextrin concentrations were investigated. A polyimide fused-silica square capillary column (75 microm I.D. x 360 microm O.D.) with a total length of 60.0 cm and effective length of 45.0 cm was used for the separation. The final BGE consisted of 20 mM phosphoric acid, 40 mM SDS and 12 mM <beta>-cyclodextrin (pH 2.2) using reverse polarity with a voltage of -17.5 kV. Samples were injected electrokinetically at -5 kV for 3 s for the qualitative analysis and hydrodynamically at 20 mbar for 0.6 s for the quantitative assay. The total run time was 22 min and the limits of detection were 3.13 microg/ml and 1.88 microg/ml for rutin and quercetin, respectively. Fingerprint profiles of the solid oral dosage forms and the results of the quantitative analysis indicated that there were major discrepancies in the marker content between products and illustrates the value of this method for use as a procedure to assess product quality of commercially available Ginkgo biloba products. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16720026/Application_of_reverse_flow_micellar_electrokinetic_chromatography_for_the_simultaneous_determination_of_flavonols_and_terpene_trilactones_in_Ginkgo_biloba_dosage_forms_ DB - PRIME DP - Unbound Medicine ER -