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Keratan sulfate and chondroitin/dermatan sulfate in maximally recovered hypocellular stromal interface scars of postmortem human LASIK corneas.
Invest Ophthalmol Vis Sci. 2006 Jun; 47(6):2390-6.IO

Abstract

PURPOSE

To analyze the amounts and distributions of nonsulfated and sulfated keratan sulfate (KS) and chondroitin/dermatan sulfate (CS/DS) disaccharides in the interface wound of human postmortem LASIK corneas in comparison with normal control corneas.

METHODS

Corneal stromal tissue samples from central and paracentral hypocellular primitive stromal interface scars of human LASIK corneas and from similar regions of normal control corneas were collected by laser capture microdissection (LCM) and subsequently were digested with specific glycosidase enzymes. Digests were directly analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS).

RESULTS

Concentrations of both monosulfated GlcNAc(6S)-beta-1,3-Gal (MSD2) and disulfated Gal (6S)-beta-1,4-GlcNAc(6S) (DSD) KS disaccharides from the LASIK interface scars were significantly lower than in normal control corneal stromas. No significant difference was found for the concentration of nonsulfated (NSD) KS disaccharides in LASIK interface scars compared with normal controls. The concentration of DeltaUA-beta-1,3-GalNAc(6S) (Deltadi-6S) CS/DS disaccharides from the LASIK interface scar was significantly higher than normal corneal stroma, whereas concentrations of DeltaUA-beta-1,3-GalNAc(4S) (Deltadi-4S) and nonsulfated Deltadi-0S CS/DS disaccharides demonstrated no significant differences from normal corneas.

CONCLUSIONS

The profiles of KS and CS/DS disaccharides in LASIK interface scars are significantly different from those in normal cornea stromal tissue, as revealed by LCM and ESI-MS/MS.

Authors+Show Affiliations

Zhang Y
Division of Biology, Kansas State University, Manhattan, Kansas 66506-4901, USA. ytz@ksu.edu
Schmack I
No affiliation info available
Dawson DG
No affiliation info available
Grossniklaus HE
No affiliation info available
Conrad AH
No affiliation info available
Kariya Y
No affiliation info available
Suzuki K
No affiliation info available
Edelhauser HF
No affiliation info available
Conrad GW
No affiliation info available

MeSH

Chondroitin SulfatesCicatrixCorneaCorneal StromaDermatan SulfateHumansKeratan SulfateKeratomileusis, Laser In SituMiddle AgedMyopiaSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16723448

Citation

Zhang, Yuntao, et al. "Keratan Sulfate and Chondroitin/dermatan Sulfate in Maximally Recovered Hypocellular Stromal Interface Scars of Postmortem Human LASIK Corneas." Investigative Ophthalmology & Visual Science, vol. 47, no. 6, 2006, pp. 2390-6.
Zhang Y, Schmack I, Dawson DG, et al. Keratan sulfate and chondroitin/dermatan sulfate in maximally recovered hypocellular stromal interface scars of postmortem human LASIK corneas. Invest Ophthalmol Vis Sci. 2006;47(6):2390-6.
Zhang, Y., Schmack, I., Dawson, D. G., Grossniklaus, H. E., Conrad, A. H., Kariya, Y., Suzuki, K., Edelhauser, H. F., & Conrad, G. W. (2006). Keratan sulfate and chondroitin/dermatan sulfate in maximally recovered hypocellular stromal interface scars of postmortem human LASIK corneas. Investigative Ophthalmology & Visual Science, 47(6), 2390-6.
Zhang Y, et al. Keratan Sulfate and Chondroitin/dermatan Sulfate in Maximally Recovered Hypocellular Stromal Interface Scars of Postmortem Human LASIK Corneas. Invest Ophthalmol Vis Sci. 2006;47(6):2390-6. PubMed PMID: 16723448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Keratan sulfate and chondroitin/dermatan sulfate in maximally recovered hypocellular stromal interface scars of postmortem human LASIK corneas. AU - Zhang,Yuntao, AU - Schmack,Ingo, AU - Dawson,Daniel G, AU - Grossniklaus,Hans E, AU - Conrad,Abigail H, AU - Kariya,Yutaka, AU - Suzuki,Kiyoshi, AU - Edelhauser,Henry F, AU - Conrad,Gary W, PY - 2006/5/26/pubmed PY - 2006/7/4/medline PY - 2006/5/26/entrez SP - 2390 EP - 6 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 47 IS - 6 N2 - PURPOSE: To analyze the amounts and distributions of nonsulfated and sulfated keratan sulfate (KS) and chondroitin/dermatan sulfate (CS/DS) disaccharides in the interface wound of human postmortem LASIK corneas in comparison with normal control corneas. METHODS: Corneal stromal tissue samples from central and paracentral hypocellular primitive stromal interface scars of human LASIK corneas and from similar regions of normal control corneas were collected by laser capture microdissection (LCM) and subsequently were digested with specific glycosidase enzymes. Digests were directly analyzed by electrospray ionization tandem mass spectrometry (ESI-MS/MS). RESULTS: Concentrations of both monosulfated GlcNAc(6S)-beta-1,3-Gal (MSD2) and disulfated Gal (6S)-beta-1,4-GlcNAc(6S) (DSD) KS disaccharides from the LASIK interface scars were significantly lower than in normal control corneal stromas. No significant difference was found for the concentration of nonsulfated (NSD) KS disaccharides in LASIK interface scars compared with normal controls. The concentration of DeltaUA-beta-1,3-GalNAc(6S) (Deltadi-6S) CS/DS disaccharides from the LASIK interface scar was significantly higher than normal corneal stroma, whereas concentrations of DeltaUA-beta-1,3-GalNAc(4S) (Deltadi-4S) and nonsulfated Deltadi-0S CS/DS disaccharides demonstrated no significant differences from normal corneas. CONCLUSIONS: The profiles of KS and CS/DS disaccharides in LASIK interface scars are significantly different from those in normal cornea stromal tissue, as revealed by LCM and ESI-MS/MS. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/16723448/Keratan_sulfate_and_chondroitin/dermatan_sulfate_in_maximally_recovered_hypocellular_stromal_interface_scars_of_postmortem_human_LASIK_corneas_ DB - PRIME DP - Unbound Medicine ER -