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Electrochemical methods for determination of the protease inhibitor indinavir sulfate in pharmaceuticals and human serum.
Pharmazie. 2006 May; 61(5):409-13.P

Abstract

Indinavir sulfate is an inhibitor of the human immunodeficiency virus (HIV) protease. The aim of this study was to determine indinavir levels in serum and pharmaceuticals, by means of electrochemical methods using the hanging mercury drop electrode (HMDE). Indinavir exhibited irreversible cathodic waves over the pH range 2.00-12.00 in different supporting electrolytes. The current-concentration plot was rectilinear over the range from 8 x 10(-7) M to 8 x 10(-6) M with a correlation coefficient of 0.996 for differential pulse voltammetry (DPV) and 8 x 10(-7) M to 1 x 10(-5) M with correlation of 0.999 M for osteryoung square ware voltammetry (OSWV) in Britton-Robinson buffer at pH 10.00. The wave was characterized as being irreversible and diffusion-controlled. The proposed methods were fully validated and successfully applied to the determination of indinavir in capsules and spiked human serum samples with good recoveries. The repeatability and reproducibility of the methods as well as precision and accuracy (such as supporting electrolyte, serum samples) were determined. No electroactive interferences from the endogenous substances were found in serum samples.

Authors+Show Affiliations

Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Tandogan, Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16724536

Citation

Dogan, B, et al. "Electrochemical Methods for Determination of the Protease Inhibitor Indinavir Sulfate in Pharmaceuticals and Human Serum." Die Pharmazie, vol. 61, no. 5, 2006, pp. 409-13.
Dogan B, Canbaz D, Ozkan SA, et al. Electrochemical methods for determination of the protease inhibitor indinavir sulfate in pharmaceuticals and human serum. Pharmazie. 2006;61(5):409-13.
Dogan, B., Canbaz, D., Ozkan, S. A., & Uslu, B. (2006). Electrochemical methods for determination of the protease inhibitor indinavir sulfate in pharmaceuticals and human serum. Die Pharmazie, 61(5), 409-13.
Dogan B, et al. Electrochemical Methods for Determination of the Protease Inhibitor Indinavir Sulfate in Pharmaceuticals and Human Serum. Pharmazie. 2006;61(5):409-13. PubMed PMID: 16724536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical methods for determination of the protease inhibitor indinavir sulfate in pharmaceuticals and human serum. AU - Dogan,B, AU - Canbaz,D, AU - Ozkan,S A, AU - Uslu,B, PY - 2006/5/27/pubmed PY - 2006/7/6/medline PY - 2006/5/27/entrez SP - 409 EP - 13 JF - Die Pharmazie JO - Pharmazie VL - 61 IS - 5 N2 - Indinavir sulfate is an inhibitor of the human immunodeficiency virus (HIV) protease. The aim of this study was to determine indinavir levels in serum and pharmaceuticals, by means of electrochemical methods using the hanging mercury drop electrode (HMDE). Indinavir exhibited irreversible cathodic waves over the pH range 2.00-12.00 in different supporting electrolytes. The current-concentration plot was rectilinear over the range from 8 x 10(-7) M to 8 x 10(-6) M with a correlation coefficient of 0.996 for differential pulse voltammetry (DPV) and 8 x 10(-7) M to 1 x 10(-5) M with correlation of 0.999 M for osteryoung square ware voltammetry (OSWV) in Britton-Robinson buffer at pH 10.00. The wave was characterized as being irreversible and diffusion-controlled. The proposed methods were fully validated and successfully applied to the determination of indinavir in capsules and spiked human serum samples with good recoveries. The repeatability and reproducibility of the methods as well as precision and accuracy (such as supporting electrolyte, serum samples) were determined. No electroactive interferences from the endogenous substances were found in serum samples. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/16724536/Electrochemical_methods_for_determination_of_the_protease_inhibitor_indinavir_sulfate_in_pharmaceuticals_and_human_serum_ DB - PRIME DP - Unbound Medicine ER -