Tags

Type your tag names separated by a space and hit enter

Method for derivatization of ephedrine and pseudoephedrine in nonaqueous media and determination by nonaqueous capillary electrophoresis with laser induced fluorescence detection.
Biomed Chromatogr. 2006 Nov; 20(11):1150-6.BC

Abstract

A nonaqueous capillary electrophoresis with laser-induced fluorescence detection method was developed for the quantification of ephedrine and pseudoephedrine after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol in nonaqueous media. The derivatization was made in off-line mode. By a series of optimizations, a derivatization buffer composed of 40 mm ammonium acetate and 20% acetonitrile and a running buffer composed of 80 mm ammonium acetate and 3% acetic acid were applied for the derivatization and separation of ephedrine and pseudoephedrine, respectively. Linear relationships for ephedrine and pseudoephedrine were obtained in the range 1.23-19.60 mg/L (correlation coefficients 0.9970 for ephedrine and 0.9994 for pseudoephedrine), and the detection limits for ephedrine and pseudoephedrine were 0.014 and 0.011 mg/L, respectively. The method was applied to the analysis of ephedrine and pseudoephedrine in four preparations with recoveries in the range 93.9-105.1%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16729273

Citation

Dong, Yuming, et al. "Method for Derivatization of Ephedrine and Pseudoephedrine in Nonaqueous Media and Determination By Nonaqueous Capillary Electrophoresis With Laser Induced Fluorescence Detection." Biomedical Chromatography : BMC, vol. 20, no. 11, 2006, pp. 1150-6.
Dong Y, Chen Y, Chen X, et al. Method for derivatization of ephedrine and pseudoephedrine in nonaqueous media and determination by nonaqueous capillary electrophoresis with laser induced fluorescence detection. Biomed Chromatogr. 2006;20(11):1150-6.
Dong, Y., Chen, Y., Chen, X., & Hu, Z. (2006). Method for derivatization of ephedrine and pseudoephedrine in nonaqueous media and determination by nonaqueous capillary electrophoresis with laser induced fluorescence detection. Biomedical Chromatography : BMC, 20(11), 1150-6.
Dong Y, et al. Method for Derivatization of Ephedrine and Pseudoephedrine in Nonaqueous Media and Determination By Nonaqueous Capillary Electrophoresis With Laser Induced Fluorescence Detection. Biomed Chromatogr. 2006;20(11):1150-6. PubMed PMID: 16729273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Method for derivatization of ephedrine and pseudoephedrine in nonaqueous media and determination by nonaqueous capillary electrophoresis with laser induced fluorescence detection. AU - Dong,Yuming, AU - Chen,Yonglei, AU - Chen,Xingguo, AU - Hu,Zhide, PY - 2006/5/27/pubmed PY - 2007/1/9/medline PY - 2006/5/27/entrez SP - 1150 EP - 6 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 20 IS - 11 N2 - A nonaqueous capillary electrophoresis with laser-induced fluorescence detection method was developed for the quantification of ephedrine and pseudoephedrine after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol in nonaqueous media. The derivatization was made in off-line mode. By a series of optimizations, a derivatization buffer composed of 40 mm ammonium acetate and 20% acetonitrile and a running buffer composed of 80 mm ammonium acetate and 3% acetic acid were applied for the derivatization and separation of ephedrine and pseudoephedrine, respectively. Linear relationships for ephedrine and pseudoephedrine were obtained in the range 1.23-19.60 mg/L (correlation coefficients 0.9970 for ephedrine and 0.9994 for pseudoephedrine), and the detection limits for ephedrine and pseudoephedrine were 0.014 and 0.011 mg/L, respectively. The method was applied to the analysis of ephedrine and pseudoephedrine in four preparations with recoveries in the range 93.9-105.1%. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/16729273/Method_for_derivatization_of_ephedrine_and_pseudoephedrine_in_nonaqueous_media_and_determination_by_nonaqueous_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/bmc.663 DB - PRIME DP - Unbound Medicine ER -