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Lupeol ameliorates aflatoxin B1-induced peroxidative hepatic damage in rats.

Abstract

Aflatoxins are potent hepatotoxic and hepatocarcinogenic agents. Reactive oxygen species and consequent peroxidative damage caused by aflatoxin are considered to be the main mechanisms leading to hepatotoxicity. The present investigation aims at assessing the hepatoprotective effect of lupeol, a pentacyclic triterpene isolated from the stem bark of Crataeva nurvala, on aflatoxin B(1) (AFB(1))-induced hepatotoxicity in a rat model. The hepatoprotection of lupeol is compared with silymarin, a well known standard hepatoprotectant. Lactate dehydrogenase, alkaline phosphatase, alanine and aspartate aminotransferases were found to be significantly increased in the serum and decreased in the liver of AFB(1) administered (1 mg/kg body mass, orally) rats, suggesting hepatic damage. Marked increase in the lipid peroxide levels and a concomitant decrease in the enzymic (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase and glutathione-S-transferase) and nonenzymic (reduced glutathione, vitamin C and vitamin E) antioxidants in the hepatic tissue were observed in AFB(1) administered rats. Pretreatment with lupeol (100 mg/kg body mass, orally) and silymarin (100 mg/kg body mass, orally) for 7 days reverted the condition to near normalcy. Hepatoprotection by lupeol is further substantiated by the normal histologic findings as against degenerative changes in the AFB(1) administered rats. The results of this study indicate that lupeol is a potent hepatoprotectant as silymarin.

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    MeSH

    Aflatoxin B1
    Animals
    Antioxidants
    Ascorbic Acid
    Drug-Induced Liver Injury
    Glutathione
    Glutathione Transferase
    Lipid Peroxidation
    Liver
    Liver Diseases
    Male
    Oxidoreductases
    Pentacyclic Triterpenes
    Rats
    Rats, Wistar
    Silymarin
    Triterpenes
    Vitamin E

    Pub Type(s)

    Comparative Study
    Journal Article

    Language

    eng

    PubMed ID

    16730236

    Citation

    TY - JOUR T1 - Lupeol ameliorates aflatoxin B1-induced peroxidative hepatic damage in rats. AU - Preetha,S P, AU - Kanniappan,M, AU - Selvakumar,E, AU - Nagaraj,M, AU - Varalakshmi,P, Y1 - 2006/04/01/ PY - 2005/12/16/received PY - 2006/3/15/revised PY - 2006/3/25/accepted PY - 2006/4/1/aheadofprint PY - 2006/5/30/pubmed PY - 2006/9/30/medline PY - 2006/5/30/entrez SP - 333 EP - 9 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp. Biochem. Physiol. C Toxicol. Pharmacol. VL - 143 IS - 3 N2 - Aflatoxins are potent hepatotoxic and hepatocarcinogenic agents. Reactive oxygen species and consequent peroxidative damage caused by aflatoxin are considered to be the main mechanisms leading to hepatotoxicity. The present investigation aims at assessing the hepatoprotective effect of lupeol, a pentacyclic triterpene isolated from the stem bark of Crataeva nurvala, on aflatoxin B(1) (AFB(1))-induced hepatotoxicity in a rat model. The hepatoprotection of lupeol is compared with silymarin, a well known standard hepatoprotectant. Lactate dehydrogenase, alkaline phosphatase, alanine and aspartate aminotransferases were found to be significantly increased in the serum and decreased in the liver of AFB(1) administered (1 mg/kg body mass, orally) rats, suggesting hepatic damage. Marked increase in the lipid peroxide levels and a concomitant decrease in the enzymic (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase and glutathione-S-transferase) and nonenzymic (reduced glutathione, vitamin C and vitamin E) antioxidants in the hepatic tissue were observed in AFB(1) administered rats. Pretreatment with lupeol (100 mg/kg body mass, orally) and silymarin (100 mg/kg body mass, orally) for 7 days reverted the condition to near normalcy. Hepatoprotection by lupeol is further substantiated by the normal histologic findings as against degenerative changes in the AFB(1) administered rats. The results of this study indicate that lupeol is a potent hepatoprotectant as silymarin. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/16730236/Lupeol_ameliorates_aflatoxin_B1_induced_peroxidative_hepatic_damage_in_rats_ L2 - http://linkinghub.elsevier.com/retrieve/pii/S1532-0456(06)00083-4 ER -