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Different expression of lipocalin-type prostaglandin D synthase in rat epididymidis.
Anim Reprod Sci. 2007 Apr; 98(3-4):302-10.AR

Abstract

This study was designed to explore the different expression of L-PGDS (lipocalin-type prostaglandin D synthase) in rat epididymidis and to gain further insight into the potential function of L-PGDS in male reproduction. The expression of L-PGDS in rat epididymidis was assessed using real-time quantitative PCR and immunoblotting. The distribution of L-PGDS in rat epididymidis was explored by immunohistochemical methods. The result of immunohistochemistry displayed that L-PGDS was mainly distributed in epididymidis and localized within the cytoplasm and the cilia of the epithelial cells. Real-time quantitative PCR and immunoblotting showed that L-PGDS was strikingly expressed in the caput epididymidis, while a moderate to weak expression was observed in the corpus and cauda epididymidis, the level of mRNA was 0.52+/-0.02 in the caput, 0.48+/-0.03 in the corpus and 0.32+/-0.01 in the cauda epididymidis, the level of protein expression in caput, corpus and the cauda groups was 1, 0.89+/-0.03 and 0.62+/-0.01, which suggested that L-PGDS may play certain kind of role during the process of the spermatozoa maturation.

Authors+Show Affiliations

Department of Biology, Fuyang Normal College, Fuyang 230632, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16730417

Citation

Chen, De-Yu, et al. "Different Expression of Lipocalin-type Prostaglandin D Synthase in Rat Epididymidis." Animal Reproduction Science, vol. 98, no. 3-4, 2007, pp. 302-10.
Chen DY, Liu SJ, Zhu MY, et al. Different expression of lipocalin-type prostaglandin D synthase in rat epididymidis. Anim Reprod Sci. 2007;98(3-4):302-10.
Chen, D. Y., Liu, S. J., Zhu, M. Y., Li, W. Y., Cui, Y. D., & Huang, Y. F. (2007). Different expression of lipocalin-type prostaglandin D synthase in rat epididymidis. Animal Reproduction Science, 98(3-4), 302-10.
Chen DY, et al. Different Expression of Lipocalin-type Prostaglandin D Synthase in Rat Epididymidis. Anim Reprod Sci. 2007;98(3-4):302-10. PubMed PMID: 16730417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different expression of lipocalin-type prostaglandin D synthase in rat epididymidis. AU - Chen,De-Yu, AU - Liu,Sheng-Jie, AU - Zhu,Mao-Ying, AU - Li,Wen-Yong, AU - Cui,Ya-Dong, AU - Huang,Yu-Feng, Y1 - 2006/05/26/ PY - 2005/12/24/received PY - 2006/02/18/revised PY - 2006/03/07/accepted PY - 2006/5/30/pubmed PY - 2007/10/5/medline PY - 2006/5/30/entrez SP - 302 EP - 10 JF - Animal reproduction science JO - Anim Reprod Sci VL - 98 IS - 3-4 N2 - This study was designed to explore the different expression of L-PGDS (lipocalin-type prostaglandin D synthase) in rat epididymidis and to gain further insight into the potential function of L-PGDS in male reproduction. The expression of L-PGDS in rat epididymidis was assessed using real-time quantitative PCR and immunoblotting. The distribution of L-PGDS in rat epididymidis was explored by immunohistochemical methods. The result of immunohistochemistry displayed that L-PGDS was mainly distributed in epididymidis and localized within the cytoplasm and the cilia of the epithelial cells. Real-time quantitative PCR and immunoblotting showed that L-PGDS was strikingly expressed in the caput epididymidis, while a moderate to weak expression was observed in the corpus and cauda epididymidis, the level of mRNA was 0.52+/-0.02 in the caput, 0.48+/-0.03 in the corpus and 0.32+/-0.01 in the cauda epididymidis, the level of protein expression in caput, corpus and the cauda groups was 1, 0.89+/-0.03 and 0.62+/-0.01, which suggested that L-PGDS may play certain kind of role during the process of the spermatozoa maturation. SN - 0378-4320 UR - https://www.unboundmedicine.com/medline/citation/16730417/Different_expression_of_lipocalin_type_prostaglandin_D_synthase_in_rat_epididymidis_ DB - PRIME DP - Unbound Medicine ER -