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[Comparison of apoptosis of articular chondrocytes in the pathogenesis of Kashin-beck disease and primary osteoarthritis].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006 Apr; 28(2):267-70.ZY

Abstract

OBJECTIVE

To investigate chondrocyte apoptosis and expression of Fas and inducible nitric oxide synthase (iNOS) in articular cartilage in the pathogenesis of Kashin-beck disease (KBD) and primary osteoarthritis (OA).

METHODS

The collected samples of articular cartilage were divided into three groups: normal control (15 cases), KBD adults (15 cases) and OA (15 cases). Chondrocyte apoptosis was detected by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling method, and Fas and iNOS in articular cartilage were stained by immunohistochemistry.

RESULTS

The positive percentages of chondrocyte apoptosis stained in articular cartilage of KBD and OA were significantly higher than that of the control (P < 0.01), and the positive percentage of chondrocytes apoptosis in the eroded areas of articular cartilage were significantly higher than in the non-eroded areas in articular cartilage of the same patient with KBD and OA (P < 0.05). There was no significant difference in positive percentage of chondrocytes apoptosis between KBD and OA. The positive percentages of Fas and iNOS in chondrocytes were significantly higher in KBD and OA than in control (P < 0.01). Significant differences in Fas and iNOS expression between the eroded areas and non-eroded areas were seen in articular cartilage of patients with KBD and OA (P < 0.05), but such difference did not exist between KBD and OA.

CONCLUSION

Cell apoptosis seems to be associated with the pathogenesis of both KBD and OA. Fas and iNOS might mediate chondrocyte apoptosis.

Authors+Show Affiliations

Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Xi'an 710061, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16733918

Citation

Wang, Shi-jie, et al. "[Comparison of Apoptosis of Articular Chondrocytes in the Pathogenesis of Kashin-beck Disease and Primary Osteoarthritis]." Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, vol. 28, no. 2, 2006, pp. 267-70.
Wang SJ, Guo X, Ren FL, et al. [Comparison of apoptosis of articular chondrocytes in the pathogenesis of Kashin-beck disease and primary osteoarthritis]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006;28(2):267-70.
Wang, S. J., Guo, X., Ren, F. L., Zhang, Y. G., Zhang, Z. T., Zhang, F. J., & Geng, D. (2006). [Comparison of apoptosis of articular chondrocytes in the pathogenesis of Kashin-beck disease and primary osteoarthritis]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae, 28(2), 267-70.
Wang SJ, et al. [Comparison of Apoptosis of Articular Chondrocytes in the Pathogenesis of Kashin-beck Disease and Primary Osteoarthritis]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2006;28(2):267-70. PubMed PMID: 16733918.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparison of apoptosis of articular chondrocytes in the pathogenesis of Kashin-beck disease and primary osteoarthritis]. AU - Wang,Shi-jie, AU - Guo,Xiong, AU - Ren,Feng-ling, AU - Zhang,Yin-gang, AU - Zhang,Zeng-tie, AU - Zhang,Fu-jun, AU - Geng,Dong, PY - 2006/6/1/pubmed PY - 2006/7/22/medline PY - 2006/6/1/entrez SP - 267 EP - 70 JF - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae JO - Zhongguo Yi Xue Ke Xue Yuan Xue Bao VL - 28 IS - 2 N2 - OBJECTIVE: To investigate chondrocyte apoptosis and expression of Fas and inducible nitric oxide synthase (iNOS) in articular cartilage in the pathogenesis of Kashin-beck disease (KBD) and primary osteoarthritis (OA). METHODS: The collected samples of articular cartilage were divided into three groups: normal control (15 cases), KBD adults (15 cases) and OA (15 cases). Chondrocyte apoptosis was detected by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling method, and Fas and iNOS in articular cartilage were stained by immunohistochemistry. RESULTS: The positive percentages of chondrocyte apoptosis stained in articular cartilage of KBD and OA were significantly higher than that of the control (P < 0.01), and the positive percentage of chondrocytes apoptosis in the eroded areas of articular cartilage were significantly higher than in the non-eroded areas in articular cartilage of the same patient with KBD and OA (P < 0.05). There was no significant difference in positive percentage of chondrocytes apoptosis between KBD and OA. The positive percentages of Fas and iNOS in chondrocytes were significantly higher in KBD and OA than in control (P < 0.01). Significant differences in Fas and iNOS expression between the eroded areas and non-eroded areas were seen in articular cartilage of patients with KBD and OA (P < 0.05), but such difference did not exist between KBD and OA. CONCLUSION: Cell apoptosis seems to be associated with the pathogenesis of both KBD and OA. Fas and iNOS might mediate chondrocyte apoptosis. SN - 1000-503X UR - https://www.unboundmedicine.com/medline/citation/16733918/[Comparison_of_apoptosis_of_articular_chondrocytes_in_the_pathogenesis_of_Kashin_beck_disease_and_primary_osteoarthritis]_ L2 - https://medlineplus.gov/osteoarthritis.html DB - PRIME DP - Unbound Medicine ER -