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Upregulation of Th1 cytokine profile (IL-12, IL-18) in bronchoalveolar lavage fluid in patients with pulmonary sarcoidosis.
J Interferon Cytokine Res. 2006 Jun; 26(6):400-5.JI

Abstract

Sarcoidosis, a systemic granulomatous disease of unknown etiology, is characterized by a predominantly Th1 cytokine milieu, which is involved in its immunopathogenesis. The role of novel immunologic markers reflecting T cell activity of the sarcoid immunologic response needs to be determined. The present study aims to evaluate the role of the Th1 cytokine pattern by estimating the local and systemic levels of interleukin-12 (IL-12) and IL-18 in bronchoalveolar lavage fluid (BALF), induced sputum, and serum of patients with pulmonary sarcoidosis. We studied prospectively 20 patients (12 women, 8 men) of median age 46 years (range 25-65) with sarcoidosis and 10 normal subjects (5 women, 5 men) of median age 39 years (range 26-60). IL-12 and IL-18 levels were measured using ELISA kits. The IL-12 BALF levels were significantly higher in sarcoidosis patients than in healthy subjects (5.64 +/- 0.21 pg/mL vs. 5.16 +/- 0.15 pg/mL, p < 0.001). In addition, IL-18 levels were significantly increased in BALF samples (47.69 +/- 6.29 pg/mL vs. 16.73 +/- 3.00 pg/mL, p < 0.001). A statistically significant decrease in IL-12 serum levels was detected in the sarcoid population compared with controls (5.77 +/- 0.50 pg/mL vs. 7.87 +/- 2.00 pg/mL, p < 0.001). No significant differences were detected in IL-12 and IL-18 levels between patients and controls in induced sputum samples. Our data suggest a potential role of IL-12 and IL-18 in the local immunologic response in pulmonary sarcoidosis. Further large-scale studies are needed to define the precise role of IL-12 and IL-18 in the immunopathogenesis of this disorder.

Authors+Show Affiliations

Department of Thoracic Medicine, University of Crete, Heraklion, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16734560

Citation

Antoniou, Katerina M., et al. "Upregulation of Th1 Cytokine Profile (IL-12, IL-18) in Bronchoalveolar Lavage Fluid in Patients With Pulmonary Sarcoidosis." Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research, vol. 26, no. 6, 2006, pp. 400-5.
Antoniou KM, Tzouvelekis A, Alexandrakis MG, et al. Upregulation of Th1 cytokine profile (IL-12, IL-18) in bronchoalveolar lavage fluid in patients with pulmonary sarcoidosis. J Interferon Cytokine Res. 2006;26(6):400-5.
Antoniou, K. M., Tzouvelekis, A., Alexandrakis, M. G., Tsiligianni, I., Tzanakis, N., Sfiridaki, K., Rachiotis, G., Bouros, D., & Siafakas, N. M. (2006). Upregulation of Th1 cytokine profile (IL-12, IL-18) in bronchoalveolar lavage fluid in patients with pulmonary sarcoidosis. Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research, 26(6), 400-5.
Antoniou KM, et al. Upregulation of Th1 Cytokine Profile (IL-12, IL-18) in Bronchoalveolar Lavage Fluid in Patients With Pulmonary Sarcoidosis. J Interferon Cytokine Res. 2006;26(6):400-5. PubMed PMID: 16734560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulation of Th1 cytokine profile (IL-12, IL-18) in bronchoalveolar lavage fluid in patients with pulmonary sarcoidosis. AU - Antoniou,Katerina M, AU - Tzouvelekis,Argyris, AU - Alexandrakis,Michael G, AU - Tsiligianni,Ioanna, AU - Tzanakis,Nikolaos, AU - Sfiridaki,Katerina, AU - Rachiotis,George, AU - Bouros,Demosthenes, AU - Siafakas,Nikolaos M, PY - 2006/6/1/pubmed PY - 2006/8/11/medline PY - 2006/6/1/entrez SP - 400 EP - 5 JF - Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research JO - J Interferon Cytokine Res VL - 26 IS - 6 N2 - Sarcoidosis, a systemic granulomatous disease of unknown etiology, is characterized by a predominantly Th1 cytokine milieu, which is involved in its immunopathogenesis. The role of novel immunologic markers reflecting T cell activity of the sarcoid immunologic response needs to be determined. The present study aims to evaluate the role of the Th1 cytokine pattern by estimating the local and systemic levels of interleukin-12 (IL-12) and IL-18 in bronchoalveolar lavage fluid (BALF), induced sputum, and serum of patients with pulmonary sarcoidosis. We studied prospectively 20 patients (12 women, 8 men) of median age 46 years (range 25-65) with sarcoidosis and 10 normal subjects (5 women, 5 men) of median age 39 years (range 26-60). IL-12 and IL-18 levels were measured using ELISA kits. The IL-12 BALF levels were significantly higher in sarcoidosis patients than in healthy subjects (5.64 +/- 0.21 pg/mL vs. 5.16 +/- 0.15 pg/mL, p < 0.001). In addition, IL-18 levels were significantly increased in BALF samples (47.69 +/- 6.29 pg/mL vs. 16.73 +/- 3.00 pg/mL, p < 0.001). A statistically significant decrease in IL-12 serum levels was detected in the sarcoid population compared with controls (5.77 +/- 0.50 pg/mL vs. 7.87 +/- 2.00 pg/mL, p < 0.001). No significant differences were detected in IL-12 and IL-18 levels between patients and controls in induced sputum samples. Our data suggest a potential role of IL-12 and IL-18 in the local immunologic response in pulmonary sarcoidosis. Further large-scale studies are needed to define the precise role of IL-12 and IL-18 in the immunopathogenesis of this disorder. SN - 1079-9907 UR - https://www.unboundmedicine.com/medline/citation/16734560/Upregulation_of_Th1_cytokine_profile__IL_12_IL_18__in_bronchoalveolar_lavage_fluid_in_patients_with_pulmonary_sarcoidosis_ L2 - https://www.liebertpub.com/doi/10.1089/jir.2006.26.400?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -