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In vitro culture of human dermal fibroblasts on electrospun polycaprolactone collagen nanofibrous membrane.
Artif Organs. 2006 Jun; 30(6):440-6.AO

Abstract

Novel cost-effective electrospun nanofibrous membrane is established for wound dressing and allogeneic cultured dermal substitute through the cultivation of human dermal fibroblast for skin defects. Synthetic polymers are generally used for tissue engineering and drug delivery applications because of their remarkable mechanical stability and slow degradation. Polycaprolactone (PCL) is used as a bioresorbable polymer in numerous medical devices as well as for tissue engineering applications. The large surface area of the polymer nanofibers with specific modifications facilitates cell adhesion and control of their cellular functions. The objectives of this study was to fabricate electrospun nanofibrous membrane from biodegradable PCL for wound dressing and collagen-blended nanofibrous membrane, and to examine fibroblast attachment, cell proliferation, and morphology of cell matrix interaction. Results of the present investigation prove that the porous nanofibrous membrane is suitable for wound dressing and modified PCL-blended collagen nanofibrous membrane is suitable for the attachment and proliferation of fibroblast, and might have the potential to be applied in tissue engineering as a dermal substitute for the treatment of skin defects and burn wounds.

Authors+Show Affiliations

Nanoscience and Nanotechnology Initiative, Division of Bioengineering, National University of Singapore, Singapore. engjrv@nus.edu.sgNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16734595

Citation

Venugopal, Jayarama Reddy, et al. "In Vitro Culture of Human Dermal Fibroblasts On Electrospun Polycaprolactone Collagen Nanofibrous Membrane." Artificial Organs, vol. 30, no. 6, 2006, pp. 440-6.
Venugopal JR, Zhang Y, Ramakrishna S. In vitro culture of human dermal fibroblasts on electrospun polycaprolactone collagen nanofibrous membrane. Artif Organs. 2006;30(6):440-6.
Venugopal, J. R., Zhang, Y., & Ramakrishna, S. (2006). In vitro culture of human dermal fibroblasts on electrospun polycaprolactone collagen nanofibrous membrane. Artificial Organs, 30(6), 440-6.
Venugopal JR, Zhang Y, Ramakrishna S. In Vitro Culture of Human Dermal Fibroblasts On Electrospun Polycaprolactone Collagen Nanofibrous Membrane. Artif Organs. 2006;30(6):440-6. PubMed PMID: 16734595.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro culture of human dermal fibroblasts on electrospun polycaprolactone collagen nanofibrous membrane. AU - Venugopal,Jayarama Reddy, AU - Zhang,Yanzhong, AU - Ramakrishna,Seeram, PY - 2006/6/1/pubmed PY - 2006/11/2/medline PY - 2006/6/1/entrez SP - 440 EP - 6 JF - Artificial organs JO - Artif Organs VL - 30 IS - 6 N2 - Novel cost-effective electrospun nanofibrous membrane is established for wound dressing and allogeneic cultured dermal substitute through the cultivation of human dermal fibroblast for skin defects. Synthetic polymers are generally used for tissue engineering and drug delivery applications because of their remarkable mechanical stability and slow degradation. Polycaprolactone (PCL) is used as a bioresorbable polymer in numerous medical devices as well as for tissue engineering applications. The large surface area of the polymer nanofibers with specific modifications facilitates cell adhesion and control of their cellular functions. The objectives of this study was to fabricate electrospun nanofibrous membrane from biodegradable PCL for wound dressing and collagen-blended nanofibrous membrane, and to examine fibroblast attachment, cell proliferation, and morphology of cell matrix interaction. Results of the present investigation prove that the porous nanofibrous membrane is suitable for wound dressing and modified PCL-blended collagen nanofibrous membrane is suitable for the attachment and proliferation of fibroblast, and might have the potential to be applied in tissue engineering as a dermal substitute for the treatment of skin defects and burn wounds. SN - 0160-564X UR - https://www.unboundmedicine.com/medline/citation/16734595/In_vitro_culture_of_human_dermal_fibroblasts_on_electrospun_polycaprolactone_collagen_nanofibrous_membrane_ L2 - https://doi.org/10.1111/j.1525-1594.2006.00239.x DB - PRIME DP - Unbound Medicine ER -