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Effects of a high-molecular-weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis.
J Antimicrob Chemother. 2006 Aug; 58(2):439-43.JA

Abstract

BACKGROUND

Porphyromonas gingivalis is a major aetiological agent of periodontitis, a destructive disease affecting the tooth-supporting tissues. Recent reports have indicated that high-molecular-weight molecules from cranberry juice concentrate can prevent the attachment of human pathogens to host tissues.

OBJECTIVES

The aim of the present study was to investigate the effect of non-dialysable material (NDM) prepared from cranberry juice concentrate on growth, biofilm formation and adherence properties of P. gingivalis.

METHODS

The effect of cranberry NDM on biofilm formation was studied using a polystyrene microplate assay and by scanning electron microscopy. The effect of cranberry NDM on the attachment properties of P. gingivalis was evaluated by a microplate assay in which mammalian proteins were immobilized into wells.

RESULTS

Our results indicated that cranberry NDM is a potent inhibitor of biofilm formation by P. gingivalis. However, it has no effect on growth and viability of bacteria. Cranberry NDM also prevented significantly the attachment of P. gingivalis to surfaces coated with type I collagen, fibrinogen or human serum.

CONCLUSIONS

Our data suggest that cranberry constituents may have a beneficial effect for the prevention and treatment of periodontitis by reducing the capacity of P. gingivalis to colonize periodontal sites.

Authors+Show Affiliations

Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval Quebec City, Quebec, Canada, G1K 7P4.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16735419

Citation

Labrecque, Julie, et al. "Effects of a High-molecular-weight Cranberry Fraction On Growth, Biofilm Formation and Adherence of Porphyromonas Gingivalis." The Journal of Antimicrobial Chemotherapy, vol. 58, no. 2, 2006, pp. 439-43.
Labrecque J, Bodet C, Chandad F, et al. Effects of a high-molecular-weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis. J Antimicrob Chemother. 2006;58(2):439-43.
Labrecque, J., Bodet, C., Chandad, F., & Grenier, D. (2006). Effects of a high-molecular-weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis. The Journal of Antimicrobial Chemotherapy, 58(2), 439-43.
Labrecque J, et al. Effects of a High-molecular-weight Cranberry Fraction On Growth, Biofilm Formation and Adherence of Porphyromonas Gingivalis. J Antimicrob Chemother. 2006;58(2):439-43. PubMed PMID: 16735419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a high-molecular-weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis. AU - Labrecque,Julie, AU - Bodet,Charles, AU - Chandad,Fatiha, AU - Grenier,Daniel, Y1 - 2006/05/30/ PY - 2006/6/1/pubmed PY - 2006/12/12/medline PY - 2006/6/1/entrez SP - 439 EP - 43 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 58 IS - 2 N2 - BACKGROUND: Porphyromonas gingivalis is a major aetiological agent of periodontitis, a destructive disease affecting the tooth-supporting tissues. Recent reports have indicated that high-molecular-weight molecules from cranberry juice concentrate can prevent the attachment of human pathogens to host tissues. OBJECTIVES: The aim of the present study was to investigate the effect of non-dialysable material (NDM) prepared from cranberry juice concentrate on growth, biofilm formation and adherence properties of P. gingivalis. METHODS: The effect of cranberry NDM on biofilm formation was studied using a polystyrene microplate assay and by scanning electron microscopy. The effect of cranberry NDM on the attachment properties of P. gingivalis was evaluated by a microplate assay in which mammalian proteins were immobilized into wells. RESULTS: Our results indicated that cranberry NDM is a potent inhibitor of biofilm formation by P. gingivalis. However, it has no effect on growth and viability of bacteria. Cranberry NDM also prevented significantly the attachment of P. gingivalis to surfaces coated with type I collagen, fibrinogen or human serum. CONCLUSIONS: Our data suggest that cranberry constituents may have a beneficial effect for the prevention and treatment of periodontitis by reducing the capacity of P. gingivalis to colonize periodontal sites. SN - 0305-7453 UR - https://www.unboundmedicine.com/medline/citation/16735419/Effects_of_a_high_molecular_weight_cranberry_fraction_on_growth_biofilm_formation_and_adherence_of_Porphyromonas_gingivalis_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkl220 DB - PRIME DP - Unbound Medicine ER -