Tags

Type your tag names separated by a space and hit enter

Nitric oxide production by pulmonary leukocytes from induced sputum in patients with asthma and its effect on epithelial cell viability.
Arch Immunol Ther Exp (Warsz). 2006 May-Jun; 54(3):201-7.AI

Abstract

INTRODUCTION

Nitric oxide (NO) is one of many factors potentially involved in lung remodeling in asthma. The aim of the study was to assess the effect of pulmonary leukocytes from patients with bronchial asthma on alveolar epithelial cell damage in relation to NO production.

MATERIALS AND METHODS

Induced sputum samples were obtained from 25 patients with bronchial asthma and 10 healthy volunteers. Twelve asthmatics were on inhaled corticosteroid treatment and 13 were corticosteroid free. Type II-like alveolar epithelial (A549) cells were cultured for 48 h in the presence of cell-free media from a 24-h culture of leukocytes obtained from the induced sputa (IS-Su). The level of NO was measured in supernatants from the cell cultures and the viability of the A549 cells was established.

RESULTS

The levels of NO in IS-Su from corticosteroid-free asthmatics were significantly higher (p = 0.001) than those in IS-Su from healthy controls. Furthermore, NO production by A549 cells exposed to IS-Su from steroid-free asthmatics (group A) was significantly higher than that from asthmatics on corticosteroid therapy (group cA) as well as from healthy controls (p = 0.01 and p = 0.001, respectively). Lower viability of the epithelial cells exposed to IS-Su was observed in group A compared with controls (median: 72% vs. 97.5%; p < 0.001). In addition, a negative correlation (R(S) = - 0.706, p < 0.001) was found between the levels of NO produced by pulmonary leukocytes and the viability of epithelial cells.

CONCLUSIONS

The results suggest that in the course of asthma, pulmonary leukocytes may interact with alveolar epithelial cells by inducing an excessive production of NO which, in turn, may contribute to epithelium impairment.

Authors+Show Affiliations

Laboratory of Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Science, Wrocław, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16736106

Citation

Bieńkowska-Haba, Małgorzata, et al. "Nitric Oxide Production By Pulmonary Leukocytes From Induced Sputum in Patients With Asthma and Its Effect On Epithelial Cell Viability." Archivum Immunologiae Et Therapiae Experimentalis, vol. 54, no. 3, 2006, pp. 201-7.
Bieńkowska-Haba M, Liebhart J, Cembrzyńska-Nowak M. Nitric oxide production by pulmonary leukocytes from induced sputum in patients with asthma and its effect on epithelial cell viability. Arch Immunol Ther Exp (Warsz). 2006;54(3):201-7.
Bieńkowska-Haba, M., Liebhart, J., & Cembrzyńska-Nowak, M. (2006). Nitric oxide production by pulmonary leukocytes from induced sputum in patients with asthma and its effect on epithelial cell viability. Archivum Immunologiae Et Therapiae Experimentalis, 54(3), 201-7.
Bieńkowska-Haba M, Liebhart J, Cembrzyńska-Nowak M. Nitric Oxide Production By Pulmonary Leukocytes From Induced Sputum in Patients With Asthma and Its Effect On Epithelial Cell Viability. Arch Immunol Ther Exp (Warsz). 2006 May-Jun;54(3):201-7. PubMed PMID: 16736106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide production by pulmonary leukocytes from induced sputum in patients with asthma and its effect on epithelial cell viability. AU - Bieńkowska-Haba,Małgorzata, AU - Liebhart,Jerzy, AU - Cembrzyńska-Nowak,Monika, Y1 - 2006/05/31/ PY - 2005/06/01/received PY - 2006/02/06/accepted PY - 2006/6/1/pubmed PY - 2006/12/9/medline PY - 2006/6/1/entrez SP - 201 EP - 7 JF - Archivum immunologiae et therapiae experimentalis JO - Arch Immunol Ther Exp (Warsz) VL - 54 IS - 3 N2 - INTRODUCTION: Nitric oxide (NO) is one of many factors potentially involved in lung remodeling in asthma. The aim of the study was to assess the effect of pulmonary leukocytes from patients with bronchial asthma on alveolar epithelial cell damage in relation to NO production. MATERIALS AND METHODS: Induced sputum samples were obtained from 25 patients with bronchial asthma and 10 healthy volunteers. Twelve asthmatics were on inhaled corticosteroid treatment and 13 were corticosteroid free. Type II-like alveolar epithelial (A549) cells were cultured for 48 h in the presence of cell-free media from a 24-h culture of leukocytes obtained from the induced sputa (IS-Su). The level of NO was measured in supernatants from the cell cultures and the viability of the A549 cells was established. RESULTS: The levels of NO in IS-Su from corticosteroid-free asthmatics were significantly higher (p = 0.001) than those in IS-Su from healthy controls. Furthermore, NO production by A549 cells exposed to IS-Su from steroid-free asthmatics (group A) was significantly higher than that from asthmatics on corticosteroid therapy (group cA) as well as from healthy controls (p = 0.01 and p = 0.001, respectively). Lower viability of the epithelial cells exposed to IS-Su was observed in group A compared with controls (median: 72% vs. 97.5%; p < 0.001). In addition, a negative correlation (R(S) = - 0.706, p < 0.001) was found between the levels of NO produced by pulmonary leukocytes and the viability of epithelial cells. CONCLUSIONS: The results suggest that in the course of asthma, pulmonary leukocytes may interact with alveolar epithelial cells by inducing an excessive production of NO which, in turn, may contribute to epithelium impairment. SN - 0004-069X UR - https://www.unboundmedicine.com/medline/citation/16736106/Nitric_oxide_production_by_pulmonary_leukocytes_from_induced_sputum_in_patients_with_asthma_and_its_effect_on_epithelial_cell_viability_ L2 - https://dx.doi.org/10.1007/s00005-006-0025-z DB - PRIME DP - Unbound Medicine ER -