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Analysis of benzalkonium chloride by capillary electrophoresis-tandem mass spectrometry.
Electrophoresis. 2006 Jun; 27(11):2225-32.E

Abstract

Conditions for the separation and determination of benzalkonium chloride (BAC) homologues by CE with UV-detection and CE coupled to MS (IT) using electrospray as ionization source were established. The separation was performed using fused-silica capillaries of 50 microm id and 100 mM acetic acid-ammonium acetate buffer solution at pH 4.5 with 80% of ACN as carrier electrolyte. CE-MS coupling parameters were optimized and methanol-10 mM acetic acid (90:10 v/v) was selected as sheath liquid. Detection limits, based on an S/N of 3:1, were calculated, and values between 0.8 and 1.3 mg/L with CE-ESI/MS and around 0.5 mg/L with CE-ESI-MS/MS, using hydrodynamic injection (15 s, 3.5 kPa), were obtained. Good run-to-run and day-to-day precisions on concentration were achieved with RSDs lower than 8%. Quantitative analysis was carried out by the internal standard method and the calibration curves showed good linearities (r(2) > 0.98). The CE-ESI-MS/MS method was successfully applied to the analysis of BAC in different ophthalmic solutions, allowing the direct determination, identification and confirmation of the BAC homologues presented in these samples.

Authors+Show Affiliations

Para BV
Department of Analytical Chemistry, University of Barcelona, Spain.
Núñez O
No affiliation info available
Moyano E
No affiliation info available
Galceran MT
No affiliation info available

MeSH

Benzalkonium CompoundsElectrophoresis, CapillaryOphthalmic SolutionsSensitivity and SpecificitySpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16736457

Citation

Para, Bianca Veronica, et al. "Analysis of Benzalkonium Chloride By Capillary Electrophoresis-tandem Mass Spectrometry." Electrophoresis, vol. 27, no. 11, 2006, pp. 2225-32.
Para BV, Núñez O, Moyano E, et al. Analysis of benzalkonium chloride by capillary electrophoresis-tandem mass spectrometry. Electrophoresis. 2006;27(11):2225-32.
Para, B. V., Núñez, O., Moyano, E., & Galceran, M. T. (2006). Analysis of benzalkonium chloride by capillary electrophoresis-tandem mass spectrometry. Electrophoresis, 27(11), 2225-32.
Para BV, et al. Analysis of Benzalkonium Chloride By Capillary Electrophoresis-tandem Mass Spectrometry. Electrophoresis. 2006;27(11):2225-32. PubMed PMID: 16736457.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of benzalkonium chloride by capillary electrophoresis-tandem mass spectrometry. AU - Para,Bianca Veronica, AU - Núñez,Oscar, AU - Moyano,Encarnación, AU - Galceran,Maria Teresa, PY - 2006/6/1/pubmed PY - 2006/9/14/medline PY - 2006/6/1/entrez SP - 2225 EP - 32 JF - Electrophoresis JO - Electrophoresis VL - 27 IS - 11 N2 - Conditions for the separation and determination of benzalkonium chloride (BAC) homologues by CE with UV-detection and CE coupled to MS (IT) using electrospray as ionization source were established. The separation was performed using fused-silica capillaries of 50 microm id and 100 mM acetic acid-ammonium acetate buffer solution at pH 4.5 with 80% of ACN as carrier electrolyte. CE-MS coupling parameters were optimized and methanol-10 mM acetic acid (90:10 v/v) was selected as sheath liquid. Detection limits, based on an S/N of 3:1, were calculated, and values between 0.8 and 1.3 mg/L with CE-ESI/MS and around 0.5 mg/L with CE-ESI-MS/MS, using hydrodynamic injection (15 s, 3.5 kPa), were obtained. Good run-to-run and day-to-day precisions on concentration were achieved with RSDs lower than 8%. Quantitative analysis was carried out by the internal standard method and the calibration curves showed good linearities (r(2) > 0.98). The CE-ESI-MS/MS method was successfully applied to the analysis of BAC in different ophthalmic solutions, allowing the direct determination, identification and confirmation of the BAC homologues presented in these samples. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16736457/Analysis_of_benzalkonium_chloride_by_capillary_electrophoresis_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -