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A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax.
Gene. 2006 Sep 01; 379:40-50.GENE

Abstract

Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+ -ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using suppression subtractive hybridisation, we characterised 586 partial cDNA sequences encoding proteins potentially involved in the metabolism of sea bass acclimated to salt- or freshwater under experimental conditions. Using these results, we first characterised complete genomic sequence of a carbonic anhydrase and then analysed mRNA expression of genes potentially involved in osmoregulation mechanisms (Na+/K+ -ATPase, carbonic anhydrase, angiotensin-converting enzyme and claudin-3), cell-cycle regulation (secretagogin) and immune system (nephrosin) in gill and intestine of wild fish from open sea and lagoons. Our analyses indicate a strong tissue- and environmental-dependant expression pattern for all the genes studied. A transcriptomic approach such as described in the present paper provides thus a first description of genes involved in metabolic or structural functions important for coping with environmental salinity variations in a euryhaline fish like the common sea bass D. labrax. It should be supplemented by proteomics to check the direct involvement of the gene products at the protein level, and by polymorphism analyses if one is to understand population or individual fluctuations in acclimation to salinity variation.

Authors+Show Affiliations

UMR CNRS-IFREMER 5171 Génome, Populations, Interactions, Adaptation, Station Méditerranéenne de l'Environnement Littoral, 1 Quai de la Daurade, 34200 Sète, France. boutetisabelle@yahoo.frNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16737785

Citation

Boutet, I, et al. "A Transcriptomic Approach of Salinity Response in the Euryhaline Teleost, Dicentrarchus Labrax." Gene, vol. 379, 2006, pp. 40-50.
Boutet I, Long Ky CL, Bonhomme F. A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax. Gene. 2006;379:40-50.
Boutet, I., Long Ky, C. L., & Bonhomme, F. (2006). A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax. Gene, 379, 40-50.
Boutet I, Long Ky CL, Bonhomme F. A Transcriptomic Approach of Salinity Response in the Euryhaline Teleost, Dicentrarchus Labrax. Gene. 2006 Sep 1;379:40-50. PubMed PMID: 16737785.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax. AU - Boutet,I, AU - Long Ky,C L, AU - Bonhomme,F, Y1 - 2006/05/04/ PY - 2005/11/04/received PY - 2006/04/11/revised PY - 2006/04/17/accepted PY - 2006/6/2/pubmed PY - 2006/11/11/medline PY - 2006/6/2/entrez SP - 40 EP - 50 JF - Gene JO - Gene VL - 379 N2 - Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+ -ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using suppression subtractive hybridisation, we characterised 586 partial cDNA sequences encoding proteins potentially involved in the metabolism of sea bass acclimated to salt- or freshwater under experimental conditions. Using these results, we first characterised complete genomic sequence of a carbonic anhydrase and then analysed mRNA expression of genes potentially involved in osmoregulation mechanisms (Na+/K+ -ATPase, carbonic anhydrase, angiotensin-converting enzyme and claudin-3), cell-cycle regulation (secretagogin) and immune system (nephrosin) in gill and intestine of wild fish from open sea and lagoons. Our analyses indicate a strong tissue- and environmental-dependant expression pattern for all the genes studied. A transcriptomic approach such as described in the present paper provides thus a first description of genes involved in metabolic or structural functions important for coping with environmental salinity variations in a euryhaline fish like the common sea bass D. labrax. It should be supplemented by proteomics to check the direct involvement of the gene products at the protein level, and by polymorphism analyses if one is to understand population or individual fluctuations in acclimation to salinity variation. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/16737785/A_transcriptomic_approach_of_salinity_response_in_the_euryhaline_teleost_Dicentrarchus_labrax_ DB - PRIME DP - Unbound Medicine ER -