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HPV16 DNA in histologically confirmed tumour-free neck lymph nodes of head and neck cancers.
Anticancer Res. 2006 Jan-Feb; 26(1B):663-70.AR

Abstract

BACKGROUND

Human papillomavirus (HPV) has been demonstrated in lymph node neck metastases (NM) of HPV-positive squamous cell carcinomas of the head and neck (HNSCC), underscoring the possible role of HPV for HNSCC progression. Reports on HPV infections in histopathologically tumour-free lymph-nodes of the SCC of the uterine cervix developing higher rates of lymph-node metastases and recurrences later in the survey of the patients was the starting point of the present study.

MATERIALS AND METHODS

The presence of HPV-DNA in primary tumours (PT, n=45), NM (n=45) and histologically confirmed tumour-free neck lymph-nodes (LN, n=102) of HNSCC from 60 patients was analysed by PCR and Southern blot hybridisation.

RESULTS

A highly positive correlation of simultaneous HPV-DNA detection in PT and NM was demonstrated. In the case of HPV-positivity of PT and/or NM [24/60 cases (40%)], 11/24 (45.8%) LN contained HPV-DNA, as well. Accepting HPV demonstration as a marker for the presence of micro-metastasis, HPV analysis would result in an upstaging of the N category in 4 out of these 11 patients.

CONCLUSION

Considering the high agreement of HPV-DNA detection in PT and simultaneous HPV-DNA demonstration in the draining NM corroborating the monoclonal character of the tumour cells, the HPV-DNA presence in LN seems to be indicative of micro-metastasis in these lymph nodes. Thus, HPV analysis might be another powerful tool for the definition of the N-status of HPV-positive HNSCC.

Authors+Show Affiliations

Department of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University Kiel, Germany. mhoffmann@hno.uni-kiel.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16739336

Citation

Hoffmann, Markus, et al. "HPV16 DNA in Histologically Confirmed Tumour-free Neck Lymph Nodes of Head and Neck Cancers." Anticancer Research, vol. 26, no. 1B, 2006, pp. 663-70.
Hoffmann M, Orlamünder A, Sucher J, et al. HPV16 DNA in histologically confirmed tumour-free neck lymph nodes of head and neck cancers. Anticancer Res. 2006;26(1B):663-70.
Hoffmann, M., Orlamünder, A., Sucher, J., Gottschlich, S., Görögh, T., Fazel, A., Ambrosch, P., Rittgen, W., Schwarz, E., & Kahn, T. (2006). HPV16 DNA in histologically confirmed tumour-free neck lymph nodes of head and neck cancers. Anticancer Research, 26(1B), 663-70.
Hoffmann M, et al. HPV16 DNA in Histologically Confirmed Tumour-free Neck Lymph Nodes of Head and Neck Cancers. Anticancer Res. 2006;26(1B):663-70. PubMed PMID: 16739336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPV16 DNA in histologically confirmed tumour-free neck lymph nodes of head and neck cancers. AU - Hoffmann,Markus, AU - Orlamünder,Antje, AU - Sucher,Jan, AU - Gottschlich,Stefan, AU - Görögh,Tibor, AU - Fazel,Asita, AU - Ambrosch,Petra, AU - Rittgen,Werner, AU - Schwarz,Elisabeth, AU - Kahn,Tomas, PY - 2006/6/3/pubmed PY - 2006/6/13/medline PY - 2006/6/3/entrez SP - 663 EP - 70 JF - Anticancer research JO - Anticancer Res. VL - 26 IS - 1B N2 - BACKGROUND: Human papillomavirus (HPV) has been demonstrated in lymph node neck metastases (NM) of HPV-positive squamous cell carcinomas of the head and neck (HNSCC), underscoring the possible role of HPV for HNSCC progression. Reports on HPV infections in histopathologically tumour-free lymph-nodes of the SCC of the uterine cervix developing higher rates of lymph-node metastases and recurrences later in the survey of the patients was the starting point of the present study. MATERIALS AND METHODS: The presence of HPV-DNA in primary tumours (PT, n=45), NM (n=45) and histologically confirmed tumour-free neck lymph-nodes (LN, n=102) of HNSCC from 60 patients was analysed by PCR and Southern blot hybridisation. RESULTS: A highly positive correlation of simultaneous HPV-DNA detection in PT and NM was demonstrated. In the case of HPV-positivity of PT and/or NM [24/60 cases (40%)], 11/24 (45.8%) LN contained HPV-DNA, as well. Accepting HPV demonstration as a marker for the presence of micro-metastasis, HPV analysis would result in an upstaging of the N category in 4 out of these 11 patients. CONCLUSION: Considering the high agreement of HPV-DNA detection in PT and simultaneous HPV-DNA demonstration in the draining NM corroborating the monoclonal character of the tumour cells, the HPV-DNA presence in LN seems to be indicative of micro-metastasis in these lymph nodes. Thus, HPV analysis might be another powerful tool for the definition of the N-status of HPV-positive HNSCC. SN - 0250-7005 UR - https://www.unboundmedicine.com/medline/citation/16739336/HPV16_DNA_in_histologically_confirmed_tumour_free_neck_lymph_nodes_of_head_and_neck_cancers_ L2 - http://ar.iiarjournals.org/cgi/pmidlookup?view=long&pmid=16739336 DB - PRIME DP - Unbound Medicine ER -