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DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia.
Lancet. 1991 Jun 08; 337(8754):1378-9.Lct

Abstract

DNA amplification and silver staining were used to identify Pneumocystis carinii in bronchoscopic lavage and induced sputum samples during 51 episodes of respiratory illness in 47 subjects with HIV infection. In 20 episodes, in which the clinical diagnosis was pneumocystis pneumonia (PCP), silver stain was positive in 14 lavage samples (70%) and 7 sputum samples (35%), whereas DNA amplification was positive in 19 lavage samples (95%) and 18 sputum samples (90%). DNA amplification was positive in only 1 of 31 patients without PCP (PCP developed in this patient within 10 weeks). DNA amplification on induced sputum offers a powerful technique for diagnosis of PCP.

Authors+Show Affiliations

Wakefield AE
Department of Paediatrics, John Radcliffe Hospital, Oxford, UK.
Guiver L
No affiliation info available
Miller RF
No affiliation info available
Hopkin JM
No affiliation info available

MeSH

Acquired Immunodeficiency SyndromeBronchoalveolar Lavage FluidDNA, FungalEvaluation Studies as TopicHumansOpportunistic InfectionsPneumocystisPneumonia, PneumocystisPolymerase Chain ReactionSputum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1674765

Citation

Wakefield, A E., et al. "DNA Amplification On Induced Sputum Samples for Diagnosis of Pneumocystis Carinii Pneumonia." Lancet (London, England), vol. 337, no. 8754, 1991, pp. 1378-9.
Wakefield AE, Guiver L, Miller RF, et al. DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet. 1991;337(8754):1378-9.
Wakefield, A. E., Guiver, L., Miller, R. F., & Hopkin, J. M. (1991). DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet (London, England), 337(8754), 1378-9.
Wakefield AE, et al. DNA Amplification On Induced Sputum Samples for Diagnosis of Pneumocystis Carinii Pneumonia. Lancet. 1991 Jun 8;337(8754):1378-9. PubMed PMID: 1674765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. AU - Wakefield,A E, AU - Guiver,L, AU - Miller,R F, AU - Hopkin,J M, PY - 1991/6/8/pubmed PY - 1991/6/8/medline PY - 1991/6/8/entrez SP - 1378 EP - 9 JF - Lancet (London, England) JO - Lancet VL - 337 IS - 8754 N2 - DNA amplification and silver staining were used to identify Pneumocystis carinii in bronchoscopic lavage and induced sputum samples during 51 episodes of respiratory illness in 47 subjects with HIV infection. In 20 episodes, in which the clinical diagnosis was pneumocystis pneumonia (PCP), silver stain was positive in 14 lavage samples (70%) and 7 sputum samples (35%), whereas DNA amplification was positive in 19 lavage samples (95%) and 18 sputum samples (90%). DNA amplification was positive in only 1 of 31 patients without PCP (PCP developed in this patient within 10 weeks). DNA amplification on induced sputum offers a powerful technique for diagnosis of PCP. SN - 0140-6736 UR - https://www.unboundmedicine.com/medline/citation/1674765/DNA_amplification_on_induced_sputum_samples_for_diagnosis_of_Pneumocystis_carinii_pneumonia_ DB - PRIME DP - Unbound Medicine ER -