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Effect of myostatin on turkey myogenic satellite cells and embryonic myoblasts.

Abstract

Myostatin (GDF-8) inhibits the activation, proliferation, and differentiation of myogenic satellite cells. The relative importance of this growth factor is demonstrated in myostatin-null mice and cattle possessing defective myostatin genes. These defects result in greatly enhanced musculature. In the present study, we examined the effect of myostatin on turkey myogenic satellite cells and embryonic myoblasts. Compared with controls (P<0.05), proliferation of both turkey embryonic myoblasts and satellite cells was inhibited between 26 and 45% in serum-free medium containing 20 ng/mL myostatin. While individual turkey satellite cell clones differed in their responsiveness to myostatin, there were no significant differences in the responsiveness of fast and slow growing cells as groups (P>0.05). A slow growing clone that exhibited the greatest response to myostatin also exhibited the greatest depression of differentiation with this growth factor (P<0.05). All other turkey satellite cell clones exhibited similar responses to the differentiation depressing effects of myostatin (P>0.05). However, myostatin had no effect on differentiation of turkey embryonic myoblasts (P>0.05). When exposed to myostatin, 4 of 6 proliferating clones and all differentiating clones increased their expression of decorin, a growth inhibitor (P<0.05). The present study demonstrates that myostatin inhibits the proliferation and differentiation of satellite cells and suggests a role for decorin in myostatin action in muscle development.

Authors+Show Affiliations

Department of Animal and Range Sciences, Box 2170, ASC 101, South Dakota State University, Brookings, SD 57007-0392, USA. Douglas.McFarland@sdstate.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16750409

Citation

McFarland, Douglas C., et al. "Effect of Myostatin On Turkey Myogenic Satellite Cells and Embryonic Myoblasts." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 144, no. 4, 2006, pp. 501-8.
McFarland DC, Velleman SG, Pesall JE, et al. Effect of myostatin on turkey myogenic satellite cells and embryonic myoblasts. Comp Biochem Physiol A Mol Integr Physiol. 2006;144(4):501-8.
McFarland, D. C., Velleman, S. G., Pesall, J. E., & Liu, C. (2006). Effect of myostatin on turkey myogenic satellite cells and embryonic myoblasts. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 144(4), 501-8.
McFarland DC, et al. Effect of Myostatin On Turkey Myogenic Satellite Cells and Embryonic Myoblasts. Comp Biochem Physiol A Mol Integr Physiol. 2006;144(4):501-8. PubMed PMID: 16750409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of myostatin on turkey myogenic satellite cells and embryonic myoblasts. AU - McFarland,Douglas C, AU - Velleman,Sandra G, AU - Pesall,Jane E, AU - Liu,Caini, Y1 - 2006/04/30/ PY - 2006/01/12/received PY - 2006/04/14/revised PY - 2006/04/19/accepted PY - 2006/6/6/pubmed PY - 2006/9/26/medline PY - 2006/6/6/entrez SP - 501 EP - 8 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 144 IS - 4 N2 - Myostatin (GDF-8) inhibits the activation, proliferation, and differentiation of myogenic satellite cells. The relative importance of this growth factor is demonstrated in myostatin-null mice and cattle possessing defective myostatin genes. These defects result in greatly enhanced musculature. In the present study, we examined the effect of myostatin on turkey myogenic satellite cells and embryonic myoblasts. Compared with controls (P<0.05), proliferation of both turkey embryonic myoblasts and satellite cells was inhibited between 26 and 45% in serum-free medium containing 20 ng/mL myostatin. While individual turkey satellite cell clones differed in their responsiveness to myostatin, there were no significant differences in the responsiveness of fast and slow growing cells as groups (P>0.05). A slow growing clone that exhibited the greatest response to myostatin also exhibited the greatest depression of differentiation with this growth factor (P<0.05). All other turkey satellite cell clones exhibited similar responses to the differentiation depressing effects of myostatin (P>0.05). However, myostatin had no effect on differentiation of turkey embryonic myoblasts (P>0.05). When exposed to myostatin, 4 of 6 proliferating clones and all differentiating clones increased their expression of decorin, a growth inhibitor (P<0.05). The present study demonstrates that myostatin inhibits the proliferation and differentiation of satellite cells and suggests a role for decorin in myostatin action in muscle development. SN - 1095-6433 UR - https://www.unboundmedicine.com/medline/citation/16750409/Effect_of_myostatin_on_turkey_myogenic_satellite_cells_and_embryonic_myoblasts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(06)00211-X DB - PRIME DP - Unbound Medicine ER -