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Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens.
J Allergy Clin Immunol 2006; 117(6):1423-9JA

Abstract

BACKGROUND

Food IgE-mediated allergy to members of the Brassicaceae family has been increasingly reported.

OBJECTIVE

To characterize cabbage-Brassica oleracea var capitata-allergy and its major allergens.

METHODS

A prospective study was performed, recruiting 17 patients allergic to cabbage, and control subjects. Skin prick tests and double-blind placebo-controlled food challenges were performed. A major allergen was isolated from cabbage by RP-HPLC and characterized by N-terminal amino acid sequencing and matrix-assisted laser desorption/ionization mass spectrometry analysis. Specific IgE determinations, IgE immunoblots, and CAP-inhibition assays were also performed.

RESULTS

Skin prick test and specific IgE were positive to cabbage in all patients. Five of them referred anaphylactic reactions when eating cabbage, and in another 5 patients, cabbage allergy was further confirmed by double-blind placebo-controlled food challenge. Most of them showed associated sensitizations to mugwort pollen, mustard, and peach. A 9-kd cabbage IgE-binding protein, Bra o 3, was identified as a lipid transfer protein (LTP) with 50% of identity to peach LTP Pru p 3. Skin prick test with Bra o 3 showed positive results in 12 of 14 cases (86%). On CAP inhibition assays, Bra o 3 managed to inhibit significantly the IgE binding to cabbage, mugwort pollen, and peach. Both Bra o 3 and Pru p 3 were recognized by IgE from the patients' sera.

CONCLUSION

Bra o 3, a cabbage LTP, is a major allergen in this food, cross-reacting with mugwort pollen and with other plant foods, such as peach.

CLINICAL IMPLICATIONS

Cabbage IgE-mediated allergy is a potentially severe condition that can present with other plant food and pollen allergies.

Authors+Show Affiliations

Departamento de Biotecnología, Escuela Técnica Superior Ingenieros Agrónomos, Universidad Politécnica de Madrid, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16751008

Citation

Palacín, Aránzazu, et al. "Cabbage Lipid Transfer Protein Bra O 3 Is a Major Allergen Responsible for Cross-reactivity Between Plant Foods and Pollens." The Journal of Allergy and Clinical Immunology, vol. 117, no. 6, 2006, pp. 1423-9.
Palacín A, Cumplido J, Figueroa J, et al. Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens. J Allergy Clin Immunol. 2006;117(6):1423-9.
Palacín, A., Cumplido, J., Figueroa, J., Ahrazem, O., Sánchez-Monge, R., Carrillo, T., ... Blanco, C. (2006). Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens. The Journal of Allergy and Clinical Immunology, 117(6), pp. 1423-9.
Palacín A, et al. Cabbage Lipid Transfer Protein Bra O 3 Is a Major Allergen Responsible for Cross-reactivity Between Plant Foods and Pollens. J Allergy Clin Immunol. 2006;117(6):1423-9. PubMed PMID: 16751008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens. AU - Palacín,Aránzazu, AU - Cumplido,Jose, AU - Figueroa,Javier, AU - Ahrazem,Oussama, AU - Sánchez-Monge,Rosa, AU - Carrillo,Teresa, AU - Salcedo,Gabriel, AU - Blanco,Carlos, Y1 - 2006/03/31/ PY - 2005/10/06/received PY - 2005/12/21/revised PY - 2006/01/18/accepted PY - 2006/6/6/pubmed PY - 2006/7/20/medline PY - 2006/6/6/entrez SP - 1423 EP - 9 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 117 IS - 6 N2 - BACKGROUND: Food IgE-mediated allergy to members of the Brassicaceae family has been increasingly reported. OBJECTIVE: To characterize cabbage-Brassica oleracea var capitata-allergy and its major allergens. METHODS: A prospective study was performed, recruiting 17 patients allergic to cabbage, and control subjects. Skin prick tests and double-blind placebo-controlled food challenges were performed. A major allergen was isolated from cabbage by RP-HPLC and characterized by N-terminal amino acid sequencing and matrix-assisted laser desorption/ionization mass spectrometry analysis. Specific IgE determinations, IgE immunoblots, and CAP-inhibition assays were also performed. RESULTS: Skin prick test and specific IgE were positive to cabbage in all patients. Five of them referred anaphylactic reactions when eating cabbage, and in another 5 patients, cabbage allergy was further confirmed by double-blind placebo-controlled food challenge. Most of them showed associated sensitizations to mugwort pollen, mustard, and peach. A 9-kd cabbage IgE-binding protein, Bra o 3, was identified as a lipid transfer protein (LTP) with 50% of identity to peach LTP Pru p 3. Skin prick test with Bra o 3 showed positive results in 12 of 14 cases (86%). On CAP inhibition assays, Bra o 3 managed to inhibit significantly the IgE binding to cabbage, mugwort pollen, and peach. Both Bra o 3 and Pru p 3 were recognized by IgE from the patients' sera. CONCLUSION: Bra o 3, a cabbage LTP, is a major allergen in this food, cross-reacting with mugwort pollen and with other plant foods, such as peach. CLINICAL IMPLICATIONS: Cabbage IgE-mediated allergy is a potentially severe condition that can present with other plant food and pollen allergies. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/16751008/Cabbage_lipid_transfer_protein_Bra_o_3_is_a_major_allergen_responsible_for_cross_reactivity_between_plant_foods_and_pollens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(06)00196-5 DB - PRIME DP - Unbound Medicine ER -