Tags

Type your tag names separated by a space and hit enter

Cytokine production by bronchoalveolar lavage T lymphocytes in chronic obstructive pulmonary disease.
J Allergy Clin Immunol. 2006 Jun; 117(6):1484-92.JA

Abstract

BACKGROUND

T lymphocytes (predominantly CD8+ cells) have previously been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD).

OBJECTIVE

We sought to describe the profile of cytokine production by CD8+ and CD4+ cells isolated from bronchoalveolar lavage fluid.

METHODS

Bronchoalveolar lavage was performed in 11 patients with COPD (median FEV1, 63.3% of predicted value) and 9 healthy control subjects. CD8+ and CD4+ T cells were isolated by means of positive selection after macrophage depletion. CD8+ and CD4+ cells were activated with anti-CD3/CD28 antibodies for 60 hours before restimulation with phorbol 12-myristate 13-acetate-ionomycin and brefeldin. Three-color flow cytometry was used to simultaneously measure levels of intracellular cytokines.

RESULTS

IL-4 was expressed by a higher percentage of stimulated CD8+ T cells (TC2) compared with CD4+ T cells (TH2) in patients with COPD (P = .01). In contrast, IFN-gamma was expressed in a significantly higher percentage of stimulated CD4+ T cells (TH1) than CD8+ T cells (TC1) in the COPD group (P = .04). TNF-alpha was expressed by almost all TC1 and TH1 cells, with virtually no expression by TC2 and TH2 cells. In addition, a small number of T cells expressing TNF-alpha alone without concomitant IFN-gamma or IL-4 expression were seen in the majority of subjects. There was a higher percentage of TC2 cells in subjects with COPD compared with that seen in the control group (P = .03). Stimulation with anti-CD3/CD28 antibodies increased the percentage of TC2 cells and decreased the percentage of TH2 cells.

CONCLUSION

Our results suggest that there are increased numbers of TC2-like cytokine-expressing cells in the lungs of patients with COPD.

CLINICAL IMPLICATIONS

These cells might be a source of TH2 cytokines, which might, at least in part, explain the lung eosinophilia associated with COPD exacerbations.

Authors+Show Affiliations

Airways Disease Section, National Heart and Lung Institute, Imperial College, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16751017

Citation

Barczyk, Adam, et al. "Cytokine Production By Bronchoalveolar Lavage T Lymphocytes in Chronic Obstructive Pulmonary Disease." The Journal of Allergy and Clinical Immunology, vol. 117, no. 6, 2006, pp. 1484-92.
Barczyk A, Pierzchała W, Kon OM, et al. Cytokine production by bronchoalveolar lavage T lymphocytes in chronic obstructive pulmonary disease. J Allergy Clin Immunol. 2006;117(6):1484-92.
Barczyk, A., Pierzchała, W., Kon, O. M., Cosio, B., Adcock, I. M., & Barnes, P. J. (2006). Cytokine production by bronchoalveolar lavage T lymphocytes in chronic obstructive pulmonary disease. The Journal of Allergy and Clinical Immunology, 117(6), 1484-92.
Barczyk A, et al. Cytokine Production By Bronchoalveolar Lavage T Lymphocytes in Chronic Obstructive Pulmonary Disease. J Allergy Clin Immunol. 2006;117(6):1484-92. PubMed PMID: 16751017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine production by bronchoalveolar lavage T lymphocytes in chronic obstructive pulmonary disease. AU - Barczyk,Adam, AU - Pierzchała,Władysław, AU - Kon,Onn M, AU - Cosio,Borja, AU - Adcock,Ian M, AU - Barnes,Peter J, Y1 - 2006/04/03/ PY - 2005/06/27/received PY - 2006/01/16/revised PY - 2006/02/06/accepted PY - 2006/6/6/pubmed PY - 2006/7/20/medline PY - 2006/6/6/entrez SP - 1484 EP - 92 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 117 IS - 6 N2 - BACKGROUND: T lymphocytes (predominantly CD8+ cells) have previously been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). OBJECTIVE: We sought to describe the profile of cytokine production by CD8+ and CD4+ cells isolated from bronchoalveolar lavage fluid. METHODS: Bronchoalveolar lavage was performed in 11 patients with COPD (median FEV1, 63.3% of predicted value) and 9 healthy control subjects. CD8+ and CD4+ T cells were isolated by means of positive selection after macrophage depletion. CD8+ and CD4+ cells were activated with anti-CD3/CD28 antibodies for 60 hours before restimulation with phorbol 12-myristate 13-acetate-ionomycin and brefeldin. Three-color flow cytometry was used to simultaneously measure levels of intracellular cytokines. RESULTS: IL-4 was expressed by a higher percentage of stimulated CD8+ T cells (TC2) compared with CD4+ T cells (TH2) in patients with COPD (P = .01). In contrast, IFN-gamma was expressed in a significantly higher percentage of stimulated CD4+ T cells (TH1) than CD8+ T cells (TC1) in the COPD group (P = .04). TNF-alpha was expressed by almost all TC1 and TH1 cells, with virtually no expression by TC2 and TH2 cells. In addition, a small number of T cells expressing TNF-alpha alone without concomitant IFN-gamma or IL-4 expression were seen in the majority of subjects. There was a higher percentage of TC2 cells in subjects with COPD compared with that seen in the control group (P = .03). Stimulation with anti-CD3/CD28 antibodies increased the percentage of TC2 cells and decreased the percentage of TH2 cells. CONCLUSION: Our results suggest that there are increased numbers of TC2-like cytokine-expressing cells in the lungs of patients with COPD. CLINICAL IMPLICATIONS: These cells might be a source of TH2 cytokines, which might, at least in part, explain the lung eosinophilia associated with COPD exacerbations. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/16751017/Cytokine_production_by_bronchoalveolar_lavage_T_lymphocytes_in_chronic_obstructive_pulmonary_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(06)00325-3 DB - PRIME DP - Unbound Medicine ER -