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Effects of interleukin-11 and epidermal growth factor on residual small intestine after experimental massive small bowel resection.
Chirurgia (Bucur). 2006 Mar-Apr; 101(2):127-33.C

Abstract

Interleukin 11 (IL-11) is a multifunctional cytokine derived from bone marrow, which has a trophic effect on small bowel epithelium. This study compares the effects of IL-11 with epidermal growth factor (EGF), a growth factor known to enhance small bowel adaptation. Forty Sprague-Dawley rats (90-100g) underwent an 85% mid-small bowel resection with primary anastomosis on day 0. Rats were divided into four treatment groups: controls (group I) received bovine serum albumin (BSA), group II received IL-11, 125 microg/kg subcutaneously (SC) twice daily, group III received EGF, 0,10 microg/g SC bid, and group IV received EGF and IL-11 in the above doses. Half of the animals (five per group) were killed on day 4 of therapy, and the rest on day 8. Animals were evaluated for weight, mucosal length, and bowel wall muscle thickness on days 4 and 8, and expression of proliferating cell nuclear antigen (PCNA) in intestinal crypt and smooth muscle cells on day 8. Body weight was similar at day 4 and 8. Mucosal thickness in groups 11 (IL-11) and IV (IL-11 and EGF) was significantly increased at day 4 and 8 compared with controls (group I) and EGF (group III, P<.001). Muscle thickness was significantly increased in the EGF and combined group IV compared with the BSA controls and IL-11 groups (P < .001). Thirty-two percent of the mucosal crypt cells in group I stained positive for PCNA, whereas 51%, 53%, and 60% stained positive in groups II (IL-11), III (EGF), and IV (IL-11 and EGF), respectively. In groups I and II, 2% and 1.7% of the myocytes stained positive for PCNA, whereas 11.2% and 5.2% in group III and IV. These data suggest that IL-11 has a trophic effect on small intestinal enterocytes, causing cell proliferation and increased mucosal thickness. EGF has a more generalized effect causing proliferation of both enterocytes and myocytes. IL-11, with or without EGF may be a useful adjunct in treatment of short bowel syndrome.

Authors+Show Affiliations

2nd Department of Surgery, Semmelweis University Medical School Budapest, Hungary. lednic@kut.sote.huNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16752677

Citation

Ledniczky, G, et al. "Effects of Interleukin-11 and Epidermal Growth Factor On Residual Small Intestine After Experimental Massive Small Bowel Resection." Chirurgia (Bucharest, Romania : 1990), vol. 101, no. 2, 2006, pp. 127-33.
Ledniczky G, Fiore N, Bognár G, et al. Effects of interleukin-11 and epidermal growth factor on residual small intestine after experimental massive small bowel resection. Chirurgia (Bucur). 2006;101(2):127-33.
Ledniczky, G., Fiore, N., Bognár, G., Ondrejka, P., & Grosfeld, J. L. (2006). Effects of interleukin-11 and epidermal growth factor on residual small intestine after experimental massive small bowel resection. Chirurgia (Bucharest, Romania : 1990), 101(2), 127-33.
Ledniczky G, et al. Effects of Interleukin-11 and Epidermal Growth Factor On Residual Small Intestine After Experimental Massive Small Bowel Resection. Chirurgia (Bucur). 2006 Mar-Apr;101(2):127-33. PubMed PMID: 16752677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of interleukin-11 and epidermal growth factor on residual small intestine after experimental massive small bowel resection. AU - Ledniczky,G, AU - Fiore,N, AU - Bognár,G, AU - Ondrejka,P, AU - Grosfeld,J L, PY - 2006/6/7/pubmed PY - 2006/6/28/medline PY - 2006/6/7/entrez SP - 127 EP - 33 JF - Chirurgia (Bucharest, Romania : 1990) JO - Chirurgia (Bucur) VL - 101 IS - 2 N2 - Interleukin 11 (IL-11) is a multifunctional cytokine derived from bone marrow, which has a trophic effect on small bowel epithelium. This study compares the effects of IL-11 with epidermal growth factor (EGF), a growth factor known to enhance small bowel adaptation. Forty Sprague-Dawley rats (90-100g) underwent an 85% mid-small bowel resection with primary anastomosis on day 0. Rats were divided into four treatment groups: controls (group I) received bovine serum albumin (BSA), group II received IL-11, 125 microg/kg subcutaneously (SC) twice daily, group III received EGF, 0,10 microg/g SC bid, and group IV received EGF and IL-11 in the above doses. Half of the animals (five per group) were killed on day 4 of therapy, and the rest on day 8. Animals were evaluated for weight, mucosal length, and bowel wall muscle thickness on days 4 and 8, and expression of proliferating cell nuclear antigen (PCNA) in intestinal crypt and smooth muscle cells on day 8. Body weight was similar at day 4 and 8. Mucosal thickness in groups 11 (IL-11) and IV (IL-11 and EGF) was significantly increased at day 4 and 8 compared with controls (group I) and EGF (group III, P<.001). Muscle thickness was significantly increased in the EGF and combined group IV compared with the BSA controls and IL-11 groups (P < .001). Thirty-two percent of the mucosal crypt cells in group I stained positive for PCNA, whereas 51%, 53%, and 60% stained positive in groups II (IL-11), III (EGF), and IV (IL-11 and EGF), respectively. In groups I and II, 2% and 1.7% of the myocytes stained positive for PCNA, whereas 11.2% and 5.2% in group III and IV. These data suggest that IL-11 has a trophic effect on small intestinal enterocytes, causing cell proliferation and increased mucosal thickness. EGF has a more generalized effect causing proliferation of both enterocytes and myocytes. IL-11, with or without EGF may be a useful adjunct in treatment of short bowel syndrome. SN - 1221-9118 UR - https://www.unboundmedicine.com/medline/citation/16752677/Effects_of_interleukin_11_and_epidermal_growth_factor_on_residual_small_intestine_after_experimental_massive_small_bowel_resection_ DB - PRIME DP - Unbound Medicine ER -