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Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay.
Respir Med. 2007 Jan; 101(1):43-7.RM

Abstract

Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis.

Authors+Show Affiliations

Department of Medicine, Indiana University, 111P-IU, Indianapolis, IN 46202, USA. chage@iupui.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

16753290

Citation

Hage, Chadi A., et al. "Diagnosis of Pulmonary Histoplasmosis and Blastomycosis By Detection of Antigen in Bronchoalveolar Lavage Fluid Using an Improved Second-generation Enzyme-linked Immunoassay." Respiratory Medicine, vol. 101, no. 1, 2007, pp. 43-7.
Hage CA, Davis TE, Egan L, et al. Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay. Respir Med. 2007;101(1):43-7.
Hage, C. A., Davis, T. E., Egan, L., Parker, M., Fuller, D., Lemonte, A. M., Durkin, M., Connelly, P., Joseph Wheat, L., Blue-Hnidy, D., & Knox, K. S. (2007). Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay. Respiratory Medicine, 101(1), 43-7.
Hage CA, et al. Diagnosis of Pulmonary Histoplasmosis and Blastomycosis By Detection of Antigen in Bronchoalveolar Lavage Fluid Using an Improved Second-generation Enzyme-linked Immunoassay. Respir Med. 2007;101(1):43-7. PubMed PMID: 16753290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay. AU - Hage,Chadi A, AU - Davis,Thomas E, AU - Egan,Lindsey, AU - Parker,Michelle, AU - Fuller,Deanna, AU - Lemonte,Ann M, AU - Durkin,Michelle, AU - Connelly,Patricia, AU - Joseph Wheat,L, AU - Blue-Hnidy,Deborah, AU - Knox,Kenneth S, Y1 - 2006/06/06/ PY - 2006/03/09/received PY - 2006/04/21/accepted PY - 2006/6/7/pubmed PY - 2007/3/24/medline PY - 2006/6/7/entrez SP - 43 EP - 7 JF - Respiratory medicine JO - Respir Med VL - 101 IS - 1 N2 - Antigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis. SN - 0954-6111 UR - https://www.unboundmedicine.com/medline/citation/16753290/Diagnosis_of_pulmonary_histoplasmosis_and_blastomycosis_by_detection_of_antigen_in_bronchoalveolar_lavage_fluid_using_an_improved_second_generation_enzyme_linked_immunoassay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(06)00228-9 DB - PRIME DP - Unbound Medicine ER -