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The balance of VEGF-C and VEGFR-3 mRNA is a predictor of lymph node metastasis in non-small cell lung cancer.
Br J Cancer. 2006 Jul 03; 95(1):75-9.BJ

Abstract

A positive association between vascular endothelial growth factor-C (VEGF-C) expression and lymph node metastasis has been reported in several cancers. However, the relationship of VEGF-C and lymph node metastasis in some cancers, including non-small cell lung cancer (NSCLC), is controversial. We evaluated the VEGF-C and vascular endothelial growth factor receptor-3 (VEGFR-3) expression in NSCLC samples from patients who had undergone surgery between 1998 and 2002 using real-time quantitative RT-PCR and immunohistochemical staining. We failed to find a positive association between VEGF-C and VEGFR-3 mRNA expression and lymph node metastasis in NSCLC. An immunohistological study demonstrated that VEGF-C was expressed not only in cancer cells, but also in macrophages in NSCLC, and that VEGFR-3 was expressed in cancer cells, macrophages, type II pneumocytes and lymph vessels. The VEGF-C/VEGFR-3 ratio of the node-positive group was significantly higher than that of the node-negative group. Immunohistochemical staining showed that VEGFR-3 was mainly expressed in cancer cells. The immunoreactivity of VEGF-C and VEGFR-3 was roughly correlated to the mRNA levels of VEGF-C and VEGFR-3 in real-time PCR. VEGF-C mRNA alone has no positive association with lymph node metastasis in NSCLC. The VEGF-C/VEGFR-3 ratio was positively associated with lymph node metastasis in NSCLC. This suggests that VEGF-C promotes lymph node metastasis while being influenced by the strength of the VEGF-C autocrine loop, and the VEGF-C/VEGFR-3 ratio can be a useful predictor of lymph node metastasis in NSCLC.

Authors+Show Affiliations

Department of Oncological and Regenerative Surgery, University of Tokushima School of Medicine; 3-18-15 Kuramotocho, Tokushima 770-8503, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16755294

Citation

Takizawa, H, et al. "The Balance of VEGF-C and VEGFR-3 mRNA Is a Predictor of Lymph Node Metastasis in Non-small Cell Lung Cancer." British Journal of Cancer, vol. 95, no. 1, 2006, pp. 75-9.
Takizawa H, Kondo K, Fujino H, et al. The balance of VEGF-C and VEGFR-3 mRNA is a predictor of lymph node metastasis in non-small cell lung cancer. Br J Cancer. 2006;95(1):75-9.
Takizawa, H., Kondo, K., Fujino, H., Kenzaki, K., Miyoshi, T., Sakiyama, S., & Tangoku, A. (2006). The balance of VEGF-C and VEGFR-3 mRNA is a predictor of lymph node metastasis in non-small cell lung cancer. British Journal of Cancer, 95(1), 75-9.
Takizawa H, et al. The Balance of VEGF-C and VEGFR-3 mRNA Is a Predictor of Lymph Node Metastasis in Non-small Cell Lung Cancer. Br J Cancer. 2006 Jul 3;95(1):75-9. PubMed PMID: 16755294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The balance of VEGF-C and VEGFR-3 mRNA is a predictor of lymph node metastasis in non-small cell lung cancer. AU - Takizawa,H, AU - Kondo,K, AU - Fujino,H, AU - Kenzaki,K, AU - Miyoshi,T, AU - Sakiyama,S, AU - Tangoku,A, Y1 - 2006/06/06/ PY - 2006/6/7/pubmed PY - 2006/12/9/medline PY - 2006/6/7/entrez SP - 75 EP - 9 JF - British journal of cancer JO - Br J Cancer VL - 95 IS - 1 N2 - A positive association between vascular endothelial growth factor-C (VEGF-C) expression and lymph node metastasis has been reported in several cancers. However, the relationship of VEGF-C and lymph node metastasis in some cancers, including non-small cell lung cancer (NSCLC), is controversial. We evaluated the VEGF-C and vascular endothelial growth factor receptor-3 (VEGFR-3) expression in NSCLC samples from patients who had undergone surgery between 1998 and 2002 using real-time quantitative RT-PCR and immunohistochemical staining. We failed to find a positive association between VEGF-C and VEGFR-3 mRNA expression and lymph node metastasis in NSCLC. An immunohistological study demonstrated that VEGF-C was expressed not only in cancer cells, but also in macrophages in NSCLC, and that VEGFR-3 was expressed in cancer cells, macrophages, type II pneumocytes and lymph vessels. The VEGF-C/VEGFR-3 ratio of the node-positive group was significantly higher than that of the node-negative group. Immunohistochemical staining showed that VEGFR-3 was mainly expressed in cancer cells. The immunoreactivity of VEGF-C and VEGFR-3 was roughly correlated to the mRNA levels of VEGF-C and VEGFR-3 in real-time PCR. VEGF-C mRNA alone has no positive association with lymph node metastasis in NSCLC. The VEGF-C/VEGFR-3 ratio was positively associated with lymph node metastasis in NSCLC. This suggests that VEGF-C promotes lymph node metastasis while being influenced by the strength of the VEGF-C autocrine loop, and the VEGF-C/VEGFR-3 ratio can be a useful predictor of lymph node metastasis in NSCLC. SN - 0007-0920 UR - https://www.unboundmedicine.com/medline/citation/16755294/The_balance_of_VEGF_C_and_VEGFR_3_mRNA_is_a_predictor_of_lymph_node_metastasis_in_non_small_cell_lung_cancer_ DB - PRIME DP - Unbound Medicine ER -