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Differential expression of estrogen receptors in women with systemic lupus erythematosus.
J Rheumatol 2006; 33(6):1093-101JR

Abstract

OBJECTIVE

Systemic lupus erythematosus (SLE) is an autoimmune disease primarily affecting women. T cell activation markers (calcineurin, CD154) increase in SLE T cells cultured with estradiol 17-beta. Biological effects of estradiol are mediated through 2 receptor proteins, estrogen receptor-alpha (ER-alpha) and estrogen receptor-beta (ER-beta). We compared the amount of estrogen receptor subtypes in T cells and measured the ability of receptor agonist-specific ligands to activate marker gene expression.

METHODS

T cells were isolated from 22 female patients with SLE and 17 control women. The amount of ER subtypes was measured by immunoblotting. Some T cells were cultured with ER-alpha or ER-beta-specific agonists. Receptor activation was measured by increased expression of the T cell activation markers CD154 and calcineurin.

RESULTS

Although the amount of ER-alpha appeared to be less in SLE T cells than in control T cells, the difference was not statistically significant (p = 0.081). The quantity of ER-beta was similar in SLE and control T cells. The expression of ER-alpha or ER-beta was independent of menstrual cycle phase, age, or SLE disease activity. Calcineurin and CD154 expression increased in SLE T cells cultured in medium containing ER-alpha and ER-beta agonists.

CONCLUSION

These data indicate that both ER subtypes activate calcineurin and CD154 in SLE but not in normal T cells. Variation in the amount of ER-alpha in SLE T cells suggests this receptor subtype participates in the sensitivity of SLE T cells to estrogen.

Authors+Show Affiliations

Department of Biology, Pittsburg State University, Kansas 66762, USA. vrider@pittstate.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16755656

Citation

Rider, Virginia, et al. "Differential Expression of Estrogen Receptors in Women With Systemic Lupus Erythematosus." The Journal of Rheumatology, vol. 33, no. 6, 2006, pp. 1093-101.
Rider V, Li X, Peterson G, et al. Differential expression of estrogen receptors in women with systemic lupus erythematosus. J Rheumatol. 2006;33(6):1093-101.
Rider, V., Li, X., Peterson, G., Dawson, J., Kimler, B. F., & Abdou, N. I. (2006). Differential expression of estrogen receptors in women with systemic lupus erythematosus. The Journal of Rheumatology, 33(6), pp. 1093-101.
Rider V, et al. Differential Expression of Estrogen Receptors in Women With Systemic Lupus Erythematosus. J Rheumatol. 2006;33(6):1093-101. PubMed PMID: 16755656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of estrogen receptors in women with systemic lupus erythematosus. AU - Rider,Virginia, AU - Li,Xiaolan, AU - Peterson,Greg, AU - Dawson,Joyce, AU - Kimler,Bruce F, AU - Abdou,Nabih I, PY - 2006/6/7/pubmed PY - 2006/10/20/medline PY - 2006/6/7/entrez SP - 1093 EP - 101 JF - The Journal of rheumatology JO - J. Rheumatol. VL - 33 IS - 6 N2 - OBJECTIVE: Systemic lupus erythematosus (SLE) is an autoimmune disease primarily affecting women. T cell activation markers (calcineurin, CD154) increase in SLE T cells cultured with estradiol 17-beta. Biological effects of estradiol are mediated through 2 receptor proteins, estrogen receptor-alpha (ER-alpha) and estrogen receptor-beta (ER-beta). We compared the amount of estrogen receptor subtypes in T cells and measured the ability of receptor agonist-specific ligands to activate marker gene expression. METHODS: T cells were isolated from 22 female patients with SLE and 17 control women. The amount of ER subtypes was measured by immunoblotting. Some T cells were cultured with ER-alpha or ER-beta-specific agonists. Receptor activation was measured by increased expression of the T cell activation markers CD154 and calcineurin. RESULTS: Although the amount of ER-alpha appeared to be less in SLE T cells than in control T cells, the difference was not statistically significant (p = 0.081). The quantity of ER-beta was similar in SLE and control T cells. The expression of ER-alpha or ER-beta was independent of menstrual cycle phase, age, or SLE disease activity. Calcineurin and CD154 expression increased in SLE T cells cultured in medium containing ER-alpha and ER-beta agonists. CONCLUSION: These data indicate that both ER subtypes activate calcineurin and CD154 in SLE but not in normal T cells. Variation in the amount of ER-alpha in SLE T cells suggests this receptor subtype participates in the sensitivity of SLE T cells to estrogen. SN - 0315-162X UR - https://www.unboundmedicine.com/medline/citation/16755656/Differential_expression_of_estrogen_receptors_in_women_with_systemic_lupus_erythematosus_ L2 - http://www.jrheum.org/cgi/pmidlookup?view=long&pmid=16755656 DB - PRIME DP - Unbound Medicine ER -