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Quenching of the electrochemiluminescence of tris(2,2'-bipyridine)ruthenium(II) by ferrocene and its potential application to quantitative DNA detection.
J Am Chem Soc. 2006 Jun 14; 128(23):7572-8.JA

Abstract

Efficient and stable quenching of electrochemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(II) by oxidizing ferrocene methanol (FcMeOH) at the electrode is reported. Bimolecular energy or electron transfer between Ru(bpy)(3)(2+*) and ferrocenium (Fc(+)), the oxidized species of Fc, along with suppression of radical reactions is suggested as the mechanism for quenching ECL. Fc shows more efficient quenching of ECL compared with the known quenchers phenol and 1,1-dimethyl-4,4'-bipyridine dication (MV(2+)). The ECL quenching rate constant was 5.6 x 10(10) M(-)(1) s(-)(1). Using Fc as a quencher label on a complementary DNA sequence, an intramolecular ECL quenching in hybridized oligonucleotide strands has been realized. With essentially complete quenching efficiency, this system has the potential for application to sequence-specific DNA detection.

Authors+Show Affiliations

Cao W
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, USA.
Ferrance JP
No affiliation info available
Demas J
No affiliation info available
Landers JP
No affiliation info available

MeSH

2,2'-DipyridylBase SequenceCoordination ComplexesDNAElectrochemistryElectrodesFerrous CompoundsIndicators and ReagentsLuminescent MeasurementsMetallocenesMolecular Sequence DataSensitivity and Specificity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16756313

Citation

Cao, Weidong, et al. "Quenching of the Electrochemiluminescence of tris(2,2'-bipyridine)ruthenium(II) By Ferrocene and Its Potential Application to Quantitative DNA Detection." Journal of the American Chemical Society, vol. 128, no. 23, 2006, pp. 7572-8.
Cao W, Ferrance JP, Demas J, et al. Quenching of the electrochemiluminescence of tris(2,2'-bipyridine)ruthenium(II) by ferrocene and its potential application to quantitative DNA detection. J Am Chem Soc. 2006;128(23):7572-8.
Cao, W., Ferrance, J. P., Demas, J., & Landers, J. P. (2006). Quenching of the electrochemiluminescence of tris(2,2'-bipyridine)ruthenium(II) by ferrocene and its potential application to quantitative DNA detection. Journal of the American Chemical Society, 128(23), 7572-8.
Cao W, et al. Quenching of the Electrochemiluminescence of tris(2,2'-bipyridine)ruthenium(II) By Ferrocene and Its Potential Application to Quantitative DNA Detection. J Am Chem Soc. 2006 Jun 14;128(23):7572-8. PubMed PMID: 16756313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quenching of the electrochemiluminescence of tris(2,2'-bipyridine)ruthenium(II) by ferrocene and its potential application to quantitative DNA detection. AU - Cao,Weidong, AU - Ferrance,Jerome P, AU - Demas,James, AU - Landers,James P, PY - 2006/6/8/pubmed PY - 2007/9/11/medline PY - 2006/6/8/entrez SP - 7572 EP - 8 JF - Journal of the American Chemical Society JO - J Am Chem Soc VL - 128 IS - 23 N2 - Efficient and stable quenching of electrochemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(II) by oxidizing ferrocene methanol (FcMeOH) at the electrode is reported. Bimolecular energy or electron transfer between Ru(bpy)(3)(2+*) and ferrocenium (Fc(+)), the oxidized species of Fc, along with suppression of radical reactions is suggested as the mechanism for quenching ECL. Fc shows more efficient quenching of ECL compared with the known quenchers phenol and 1,1-dimethyl-4,4'-bipyridine dication (MV(2+)). The ECL quenching rate constant was 5.6 x 10(10) M(-)(1) s(-)(1). Using Fc as a quencher label on a complementary DNA sequence, an intramolecular ECL quenching in hybridized oligonucleotide strands has been realized. With essentially complete quenching efficiency, this system has the potential for application to sequence-specific DNA detection. SN - 0002-7863 UR - https://www.unboundmedicine.com/medline/citation/16756313/Quenching_of_the_electrochemiluminescence_of_tris_22'_bipyridine_ruthenium_II__by_ferrocene_and_its_potential_application_to_quantitative_DNA_detection_ DB - PRIME DP - Unbound Medicine ER -