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The lumbar spinal cord glial cells actively modulate subcutaneous formalin induced hyperalgesia in the rat.
Neurosci Res. 2006 Aug; 55(4):442-50.NR

Abstract

We investigated the response and relationship of glial cells and neurons in lumbar spinal cord to hyperalgesia induced by the unilateral subcutaneous formalin injection into the hindpaw of rats. It was demonstrated that Fos/NeuN immunoreactive (-IR) neurons, glial fibrillary acidic protein (GFAP)-IR astrocytes and OX42-IR microglia were distributed in dorsal horn of lumbar spinal cord, predominantly in the superficial layer. In the time-course studies, GFAP-IR astrocytes were firstly detected, OX42-IR microglia were sequentially observed, Fos/NeuN-IR neurons were found slightly late. Immunoelectron microscopy studies established that many heterotypic gap junctions (HGJs), which consisting of Cx43-IR astrocytic process on one side and Cx32-IR dendrite on the other side, were present in superficial layer of dorsal horn. Ninety-one HGJs were found in 100 areas of experimental rats and occupied 91%, while only 39% HGJs were found in control rats. In experimental rats pretreated with intrathecal (i.t.) application of the carbenoxolone (a gap junction blocker) or fluorocitrate (a glial metabolic inhibitor), the paw withdrawal thermal latency was prolonged than those application of the sterile saline (i.t.). It suggests that spinal cord glial cells may play an important role for modulation of hyperalgesia induced by noxious stimuli through HGJs which located between astrocytes and neurons.

Authors+Show Affiliations

Institute of Neurosciences, The Fourth Military Medical University, Changle West Road, Xi'an 710032, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16759728

Citation

Qin, Ming, et al. "The Lumbar Spinal Cord Glial Cells Actively Modulate Subcutaneous Formalin Induced Hyperalgesia in the Rat." Neuroscience Research, vol. 55, no. 4, 2006, pp. 442-50.
Qin M, Wang JJ, Cao R, et al. The lumbar spinal cord glial cells actively modulate subcutaneous formalin induced hyperalgesia in the rat. Neurosci Res. 2006;55(4):442-50.
Qin, M., Wang, J. J., Cao, R., Zhang, H., Duan, L., Gao, B., Xiong, Y. F., Chen, L. W., & Rao, Z. R. (2006). The lumbar spinal cord glial cells actively modulate subcutaneous formalin induced hyperalgesia in the rat. Neuroscience Research, 55(4), 442-50.
Qin M, et al. The Lumbar Spinal Cord Glial Cells Actively Modulate Subcutaneous Formalin Induced Hyperalgesia in the Rat. Neurosci Res. 2006;55(4):442-50. PubMed PMID: 16759728.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The lumbar spinal cord glial cells actively modulate subcutaneous formalin induced hyperalgesia in the rat. AU - Qin,Ming, AU - Wang,Jing-Jie, AU - Cao,Rong, AU - Zhang,Hui, AU - Duan,Li, AU - Gao,Bei, AU - Xiong,Ying-Fei, AU - Chen,Liang-Wei, AU - Rao,Zhi-Ren, Y1 - 2006/06/08/ PY - 2005/10/14/received PY - 2006/04/21/revised PY - 2006/04/26/accepted PY - 2006/6/9/pubmed PY - 2006/9/9/medline PY - 2006/6/9/entrez SP - 442 EP - 50 JF - Neuroscience research JO - Neurosci Res VL - 55 IS - 4 N2 - We investigated the response and relationship of glial cells and neurons in lumbar spinal cord to hyperalgesia induced by the unilateral subcutaneous formalin injection into the hindpaw of rats. It was demonstrated that Fos/NeuN immunoreactive (-IR) neurons, glial fibrillary acidic protein (GFAP)-IR astrocytes and OX42-IR microglia were distributed in dorsal horn of lumbar spinal cord, predominantly in the superficial layer. In the time-course studies, GFAP-IR astrocytes were firstly detected, OX42-IR microglia were sequentially observed, Fos/NeuN-IR neurons were found slightly late. Immunoelectron microscopy studies established that many heterotypic gap junctions (HGJs), which consisting of Cx43-IR astrocytic process on one side and Cx32-IR dendrite on the other side, were present in superficial layer of dorsal horn. Ninety-one HGJs were found in 100 areas of experimental rats and occupied 91%, while only 39% HGJs were found in control rats. In experimental rats pretreated with intrathecal (i.t.) application of the carbenoxolone (a gap junction blocker) or fluorocitrate (a glial metabolic inhibitor), the paw withdrawal thermal latency was prolonged than those application of the sterile saline (i.t.). It suggests that spinal cord glial cells may play an important role for modulation of hyperalgesia induced by noxious stimuli through HGJs which located between astrocytes and neurons. SN - 0168-0102 UR - https://www.unboundmedicine.com/medline/citation/16759728/The_lumbar_spinal_cord_glial_cells_actively_modulate_subcutaneous_formalin_induced_hyperalgesia_in_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-0102(06)00113-1 DB - PRIME DP - Unbound Medicine ER -